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Mapping a mutation causing pale yellow petals in Brassica rapa
Petal color is an important trait for both ornamental purposes and also for attracting pollinators. Here, we report a mutation of Brassica rapa R-o-18 with pale yellow petals that we retrieved from an EMS population and named whiter shade of pale ( wsp ). Phenotypic segregation ratio of an F2 mappin...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10242410/ https://www.ncbi.nlm.nih.gov/pubmed/37287506 http://dx.doi.org/10.17912/micropub.biology.000866 |
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author | Chow, Hiu Tung Mosher, Rebecca A |
author_facet | Chow, Hiu Tung Mosher, Rebecca A |
author_sort | Chow, Hiu Tung |
collection | PubMed |
description | Petal color is an important trait for both ornamental purposes and also for attracting pollinators. Here, we report a mutation of Brassica rapa R-o-18 with pale yellow petals that we retrieved from an EMS population and named whiter shade of pale ( wsp ). Phenotypic segregation ratio of an F2 mapping population indicates the phenotype is controlled by a single recessive gene. Mapping data from the whole genome sequencing coupled with allele frequency analysis suggests the mutation is located in a ~2 Mbp interval on chromosome 2. The interval contains a putative esterase/lipase/thioesterase protein previously demonstrated to account for floral color in B. rapa . We demonstrate that wsp carries a G to A missense mutation causing an aspartate to asparagine substitution within the putative lysophospholipid acyltransferase domain. |
format | Online Article Text |
id | pubmed-10242410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-102424102023-06-07 Mapping a mutation causing pale yellow petals in Brassica rapa Chow, Hiu Tung Mosher, Rebecca A MicroPubl Biol New Finding Petal color is an important trait for both ornamental purposes and also for attracting pollinators. Here, we report a mutation of Brassica rapa R-o-18 with pale yellow petals that we retrieved from an EMS population and named whiter shade of pale ( wsp ). Phenotypic segregation ratio of an F2 mapping population indicates the phenotype is controlled by a single recessive gene. Mapping data from the whole genome sequencing coupled with allele frequency analysis suggests the mutation is located in a ~2 Mbp interval on chromosome 2. The interval contains a putative esterase/lipase/thioesterase protein previously demonstrated to account for floral color in B. rapa . We demonstrate that wsp carries a G to A missense mutation causing an aspartate to asparagine substitution within the putative lysophospholipid acyltransferase domain. Caltech Library 2023-05-22 /pmc/articles/PMC10242410/ /pubmed/37287506 http://dx.doi.org/10.17912/micropub.biology.000866 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Chow, Hiu Tung Mosher, Rebecca A Mapping a mutation causing pale yellow petals in Brassica rapa |
title |
Mapping a mutation causing pale yellow petals in
Brassica rapa
|
title_full |
Mapping a mutation causing pale yellow petals in
Brassica rapa
|
title_fullStr |
Mapping a mutation causing pale yellow petals in
Brassica rapa
|
title_full_unstemmed |
Mapping a mutation causing pale yellow petals in
Brassica rapa
|
title_short |
Mapping a mutation causing pale yellow petals in
Brassica rapa
|
title_sort | mapping a mutation causing pale yellow petals in
brassica rapa |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10242410/ https://www.ncbi.nlm.nih.gov/pubmed/37287506 http://dx.doi.org/10.17912/micropub.biology.000866 |
work_keys_str_mv | AT chowhiutung mappingamutationcausingpaleyellowpetalsinbrassicarapa AT mosherrebeccaa mappingamutationcausingpaleyellowpetalsinbrassicarapa |