Cargando…

Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry

β-Nerve growth factor (NGF) is a neurotrophin that plays a critical role in fetal development during gestation. ProNGF is the precursor form of NGF with a distinct biological profile. In order to investigate the role of NGF and proNGF in pregnant human females, a sensitive and selective immunoaffini...

Descripción completa

Detalles Bibliográficos
Autores principales: Walsh, Jason, Palandra, Joe, Goihberg, Eduward, Deng, Shibing, Hurst, Susan, Neubert, Hendrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244379/
https://www.ncbi.nlm.nih.gov/pubmed/37280257
http://dx.doi.org/10.1038/s41598-023-34695-7
_version_ 1785054626882519040
author Walsh, Jason
Palandra, Joe
Goihberg, Eduward
Deng, Shibing
Hurst, Susan
Neubert, Hendrik
author_facet Walsh, Jason
Palandra, Joe
Goihberg, Eduward
Deng, Shibing
Hurst, Susan
Neubert, Hendrik
author_sort Walsh, Jason
collection PubMed
description β-Nerve growth factor (NGF) is a neurotrophin that plays a critical role in fetal development during gestation. ProNGF is the precursor form of NGF with a distinct biological profile. In order to investigate the role of NGF and proNGF in pregnant human females, a sensitive and selective immunoaffinity liquid chromatography-tandem mass spectrometry assay was developed and qualified to simultaneously measure the levels of total NGF (tNGF; sum of mature and proNGF) and proNGF using full and relative quantification strategies, respectively. The assay was used to determine serum tNGF and proNGF levels in the three gestational trimesters of pregnancy and in non-pregnant female controls. Mean tNGF ± SD were 44.6 ± 12.3, 42.6 ± 9.3, 65.4 ± 17.6 and 77.0 ± 17.8 pg/mL for non-pregnant, first, second, and third trimesters, respectively, demonstrating no significant increase in circulating tNGF between the control and the first trimester, and a moderate yet significant 1.7-fold increase through gestation. proNGF levels during the first trimester were unchanged compared to control. In contrast to tNGF, however, proNGF levels during gestation remained stable without significant changes. The development of this sensitive, novel immunoaffinity duplexed assay for both tNGF and proNGF is expected to enable further elucidation of the roles these neurotrophins play in human pregnancy as well as other models.
format Online
Article
Text
id pubmed-10244379
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-102443792023-06-08 Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry Walsh, Jason Palandra, Joe Goihberg, Eduward Deng, Shibing Hurst, Susan Neubert, Hendrik Sci Rep Article β-Nerve growth factor (NGF) is a neurotrophin that plays a critical role in fetal development during gestation. ProNGF is the precursor form of NGF with a distinct biological profile. In order to investigate the role of NGF and proNGF in pregnant human females, a sensitive and selective immunoaffinity liquid chromatography-tandem mass spectrometry assay was developed and qualified to simultaneously measure the levels of total NGF (tNGF; sum of mature and proNGF) and proNGF using full and relative quantification strategies, respectively. The assay was used to determine serum tNGF and proNGF levels in the three gestational trimesters of pregnancy and in non-pregnant female controls. Mean tNGF ± SD were 44.6 ± 12.3, 42.6 ± 9.3, 65.4 ± 17.6 and 77.0 ± 17.8 pg/mL for non-pregnant, first, second, and third trimesters, respectively, demonstrating no significant increase in circulating tNGF between the control and the first trimester, and a moderate yet significant 1.7-fold increase through gestation. proNGF levels during the first trimester were unchanged compared to control. In contrast to tNGF, however, proNGF levels during gestation remained stable without significant changes. The development of this sensitive, novel immunoaffinity duplexed assay for both tNGF and proNGF is expected to enable further elucidation of the roles these neurotrophins play in human pregnancy as well as other models. Nature Publishing Group UK 2023-06-06 /pmc/articles/PMC10244379/ /pubmed/37280257 http://dx.doi.org/10.1038/s41598-023-34695-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Walsh, Jason
Palandra, Joe
Goihberg, Eduward
Deng, Shibing
Hurst, Susan
Neubert, Hendrik
Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
title Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
title_full Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
title_fullStr Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
title_full_unstemmed Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
title_short Analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
title_sort analysis of β-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244379/
https://www.ncbi.nlm.nih.gov/pubmed/37280257
http://dx.doi.org/10.1038/s41598-023-34695-7
work_keys_str_mv AT walshjason analysisofbnervegrowthfactoranditsprecursorduringhumanpregnancybyimmunoaffinityliquidchromatographytandemmassspectrometry
AT palandrajoe analysisofbnervegrowthfactoranditsprecursorduringhumanpregnancybyimmunoaffinityliquidchromatographytandemmassspectrometry
AT goihbergeduward analysisofbnervegrowthfactoranditsprecursorduringhumanpregnancybyimmunoaffinityliquidchromatographytandemmassspectrometry
AT dengshibing analysisofbnervegrowthfactoranditsprecursorduringhumanpregnancybyimmunoaffinityliquidchromatographytandemmassspectrometry
AT hurstsusan analysisofbnervegrowthfactoranditsprecursorduringhumanpregnancybyimmunoaffinityliquidchromatographytandemmassspectrometry
AT neuberthendrik analysisofbnervegrowthfactoranditsprecursorduringhumanpregnancybyimmunoaffinityliquidchromatographytandemmassspectrometry