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Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus)
This study investigated the clinical and pathological symptoms of waterborne lead toxicity in wild Nile tilapia collected from a lead-contaminated area (the Mariotteya Canal: Pb = 0.6 ± 0.21 mg L(−1)) and a farmed fish after 2 weeks of experimental exposure to lead acetate (5–10 mg L(−1)) in additio...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244493/ https://www.ncbi.nlm.nih.gov/pubmed/37280317 http://dx.doi.org/10.1038/s41598-023-36121-4 |
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author | Abu-Elala, Nermeen M. Khattab, Marwa S. AbuBakr, Huda O. Helmy, Samah Hesham, Ahmed Younis, Nehal A. Dawood, Mahmoud A. O. El Basuini, Mohammed F. |
author_facet | Abu-Elala, Nermeen M. Khattab, Marwa S. AbuBakr, Huda O. Helmy, Samah Hesham, Ahmed Younis, Nehal A. Dawood, Mahmoud A. O. El Basuini, Mohammed F. |
author_sort | Abu-Elala, Nermeen M. |
collection | PubMed |
description | This study investigated the clinical and pathological symptoms of waterborne lead toxicity in wild Nile tilapia collected from a lead-contaminated area (the Mariotteya Canal: Pb = 0.6 ± 0.21 mg L(−1)) and a farmed fish after 2 weeks of experimental exposure to lead acetate (5–10 mg L(−1)) in addition to evaluating the efficacy of neem leaf powder (NLP) treatment in mitigating symptoms of lead toxicity. A total of 150 fish (20 ± 2 g) were alienated into five groups (30 fish/group with three replicates). G1 was assigned as a negative control without any treatments. Groups (2–5) were exposed to lead acetate for 2 weeks at a concentration of 5 mg L(−1) (G2 and G3) or 10 mg L(−1) (G4 and G5). During the lead exposure period, all groups were reared under the same conditions, while G3 and G5 were treated with 1 g L(−1) NLP. Lead toxicity induced DNA fragmentation and lipid peroxidation and decreased the level of glutathione and expression of heme synthesis enzyme delta aminolaevulinic acid dehydratase (ALA-D) in wild tilapia, G2, and G4. NLP could alleviate the oxidative stress stimulated by lead in G3 and showed an insignificant effect in G5. The pathological findings, including epithelial hyperplasia in the gills, edema in the gills and muscles, degeneration and necrosis in the liver and muscle, and leukocytic infiltration in all organs, were directly correlated with lead concentration. Thus, the aqueous application of NLP at 1 g L(−1) reduced oxidative stress and lowered the pathological alterations induced by lead toxicity. |
format | Online Article Text |
id | pubmed-10244493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102444932023-06-08 Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) Abu-Elala, Nermeen M. Khattab, Marwa S. AbuBakr, Huda O. Helmy, Samah Hesham, Ahmed Younis, Nehal A. Dawood, Mahmoud A. O. El Basuini, Mohammed F. Sci Rep Article This study investigated the clinical and pathological symptoms of waterborne lead toxicity in wild Nile tilapia collected from a lead-contaminated area (the Mariotteya Canal: Pb = 0.6 ± 0.21 mg L(−1)) and a farmed fish after 2 weeks of experimental exposure to lead acetate (5–10 mg L(−1)) in addition to evaluating the efficacy of neem leaf powder (NLP) treatment in mitigating symptoms of lead toxicity. A total of 150 fish (20 ± 2 g) were alienated into five groups (30 fish/group with three replicates). G1 was assigned as a negative control without any treatments. Groups (2–5) were exposed to lead acetate for 2 weeks at a concentration of 5 mg L(−1) (G2 and G3) or 10 mg L(−1) (G4 and G5). During the lead exposure period, all groups were reared under the same conditions, while G3 and G5 were treated with 1 g L(−1) NLP. Lead toxicity induced DNA fragmentation and lipid peroxidation and decreased the level of glutathione and expression of heme synthesis enzyme delta aminolaevulinic acid dehydratase (ALA-D) in wild tilapia, G2, and G4. NLP could alleviate the oxidative stress stimulated by lead in G3 and showed an insignificant effect in G5. The pathological findings, including epithelial hyperplasia in the gills, edema in the gills and muscles, degeneration and necrosis in the liver and muscle, and leukocytic infiltration in all organs, were directly correlated with lead concentration. Thus, the aqueous application of NLP at 1 g L(−1) reduced oxidative stress and lowered the pathological alterations induced by lead toxicity. Nature Publishing Group UK 2023-06-06 /pmc/articles/PMC10244493/ /pubmed/37280317 http://dx.doi.org/10.1038/s41598-023-36121-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Abu-Elala, Nermeen M. Khattab, Marwa S. AbuBakr, Huda O. Helmy, Samah Hesham, Ahmed Younis, Nehal A. Dawood, Mahmoud A. O. El Basuini, Mohammed F. Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) |
title | Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) |
title_full | Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) |
title_fullStr | Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed | Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) |
title_short | Neem leaf powder (Azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in Nile tilapia (Oreochromis niloticus) |
title_sort | neem leaf powder (azadirachta indica) mitigates oxidative stress and pathological alterations triggered by lead toxicity in nile tilapia (oreochromis niloticus) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244493/ https://www.ncbi.nlm.nih.gov/pubmed/37280317 http://dx.doi.org/10.1038/s41598-023-36121-4 |
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