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Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice

Antimicrobial resistance poses a significant threat to public health and social development worldwide. This study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections. Eco-friendly spherical AgNPs were synthesized using rutin at...

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Autores principales: Du, Huamao, Wang, Xiaoling, Zhang, Hongying, Chen, Heming, Deng, Xiaoyu, He, Yujing, Tang, Huaze, Deng, Fuchang, Ren, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244497/
https://www.ncbi.nlm.nih.gov/pubmed/37293234
http://dx.doi.org/10.3389/fmicb.2023.1153147
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author Du, Huamao
Wang, Xiaoling
Zhang, Hongying
Chen, Heming
Deng, Xiaoyu
He, Yujing
Tang, Huaze
Deng, Fuchang
Ren, Zhihong
author_facet Du, Huamao
Wang, Xiaoling
Zhang, Hongying
Chen, Heming
Deng, Xiaoyu
He, Yujing
Tang, Huaze
Deng, Fuchang
Ren, Zhihong
author_sort Du, Huamao
collection PubMed
description Antimicrobial resistance poses a significant threat to public health and social development worldwide. This study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections. Eco-friendly spherical AgNPs were synthesized using rutin at room temperature. The biocompatibility of both polyvinyl pyrrolidone (PVP) and mouse serum (MS)-stabilized AgNPs was evaluated at 20 μg/mL and showed a similar distribution in mice. However, only MS-AgNPs significantly protected mice from sepsis caused by the multidrug-resistant Escherichia coli (E. coli) CQ10 strain (p = 0.039). The data revealed that MS-AgNPs facilitated the elimination of Escherichia coli (E. coli) in the blood and the spleen, and the mice experienced only a mild inflammatory response, as interleukin-6, tumor necrosis factor-α, chemokine KC, and C-reactive protein levels were significantly lower than those in the control group. The results suggest that the plasma protein corona strengthens the antibacterial effect of AgNPs in vivo and may be a potential strategy for combating antimicrobial resistance.
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spelling pubmed-102444972023-06-08 Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice Du, Huamao Wang, Xiaoling Zhang, Hongying Chen, Heming Deng, Xiaoyu He, Yujing Tang, Huaze Deng, Fuchang Ren, Zhihong Front Microbiol Microbiology Antimicrobial resistance poses a significant threat to public health and social development worldwide. This study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections. Eco-friendly spherical AgNPs were synthesized using rutin at room temperature. The biocompatibility of both polyvinyl pyrrolidone (PVP) and mouse serum (MS)-stabilized AgNPs was evaluated at 20 μg/mL and showed a similar distribution in mice. However, only MS-AgNPs significantly protected mice from sepsis caused by the multidrug-resistant Escherichia coli (E. coli) CQ10 strain (p = 0.039). The data revealed that MS-AgNPs facilitated the elimination of Escherichia coli (E. coli) in the blood and the spleen, and the mice experienced only a mild inflammatory response, as interleukin-6, tumor necrosis factor-α, chemokine KC, and C-reactive protein levels were significantly lower than those in the control group. The results suggest that the plasma protein corona strengthens the antibacterial effect of AgNPs in vivo and may be a potential strategy for combating antimicrobial resistance. Frontiers Media S.A. 2023-05-24 /pmc/articles/PMC10244497/ /pubmed/37293234 http://dx.doi.org/10.3389/fmicb.2023.1153147 Text en Copyright © 2023 Du, Wang, Zhang, Chen, Deng, He, Tang, Deng and Ren. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Du, Huamao
Wang, Xiaoling
Zhang, Hongying
Chen, Heming
Deng, Xiaoyu
He, Yujing
Tang, Huaze
Deng, Fuchang
Ren, Zhihong
Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
title Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
title_full Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
title_fullStr Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
title_full_unstemmed Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
title_short Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant Escherichia coli infections in mice
title_sort serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistant escherichia coli infections in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244497/
https://www.ncbi.nlm.nih.gov/pubmed/37293234
http://dx.doi.org/10.3389/fmicb.2023.1153147
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