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Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods

Spider mites (Acari: Tetranychidae) are dangerous pests of agricultural and ornamental crops, the most economically significant of them belonging to the genera Tetranychus, Eutetranychus, Oligonychus and Panonychus. The expansion of the distribution areas, the increased harmfulness and dangerous sta...

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Autores principales: Razuvaeva, A.V., Ulyanova, E.G., Skolotneva, E.S., Andreeva, I.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244583/
https://www.ncbi.nlm.nih.gov/pubmed/37293445
http://dx.doi.org/10.18699/VJGB-23-30
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author Razuvaeva, A.V.
Ulyanova, E.G.
Skolotneva, E.S.
Andreeva, I.V.
author_facet Razuvaeva, A.V.
Ulyanova, E.G.
Skolotneva, E.S.
Andreeva, I.V.
author_sort Razuvaeva, A.V.
collection PubMed
description Spider mites (Acari: Tetranychidae) are dangerous pests of agricultural and ornamental crops, the most economically significant of them belonging to the genera Tetranychus, Eutetranychus, Oligonychus and Panonychus. The expansion of the distribution areas, the increased harmfulness and dangerous status of certain species in the family Tetranychidae and their invasion of new regions pose a serious threat to the phytosanitary status of agro- and biocenoses. Various approaches to acarofauna species diagnosis determine a rather diverse range of currently existing methods generally described in this review. Identification of spider mites by morphological traits, which is currently considered the main method, is complicated due to the complexity of preparing biomaterials for diagnosis and a limited number of diagnostic signs. In this regard, biochemical and molecular genetic methods such as allozyme analysis, DNA barcoding, restriction fragment length polymorphism (PCR-RFLP), selection of species-specific primers and real-time PCR are becoming important. In the review, close attention is paid to the successful use of these methods for species discrimination in the mites of the subfamily Tetranychinae. For some species, e. g., the two-spotted spider mite (Tetranychus urticae), a range of identification methods has been developed – from allozyme analysis to loop isothermal amplification (LAMP), while for many other species a much smaller variety of approaches is available. The greatest accuracy in the identification of spider mites can be achieved using a combination of several methods, e. g., examination of morphological features and one of the molecular approaches (DNA barcoding, PCR-RFLP, etc.). This review may be useful to specialists who are in search of an effective system for spider mite species identification as well as when developing new test systems relevant to specific plant crops or a specific region
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spelling pubmed-102445832023-06-08 Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods Razuvaeva, A.V. Ulyanova, E.G. Skolotneva, E.S. Andreeva, I.V. Vavilovskii Zhurnal Genet Selektsii Review Spider mites (Acari: Tetranychidae) are dangerous pests of agricultural and ornamental crops, the most economically significant of them belonging to the genera Tetranychus, Eutetranychus, Oligonychus and Panonychus. The expansion of the distribution areas, the increased harmfulness and dangerous status of certain species in the family Tetranychidae and their invasion of new regions pose a serious threat to the phytosanitary status of agro- and biocenoses. Various approaches to acarofauna species diagnosis determine a rather diverse range of currently existing methods generally described in this review. Identification of spider mites by morphological traits, which is currently considered the main method, is complicated due to the complexity of preparing biomaterials for diagnosis and a limited number of diagnostic signs. In this regard, biochemical and molecular genetic methods such as allozyme analysis, DNA barcoding, restriction fragment length polymorphism (PCR-RFLP), selection of species-specific primers and real-time PCR are becoming important. In the review, close attention is paid to the successful use of these methods for species discrimination in the mites of the subfamily Tetranychinae. For some species, e. g., the two-spotted spider mite (Tetranychus urticae), a range of identification methods has been developed – from allozyme analysis to loop isothermal amplification (LAMP), while for many other species a much smaller variety of approaches is available. The greatest accuracy in the identification of spider mites can be achieved using a combination of several methods, e. g., examination of morphological features and one of the molecular approaches (DNA barcoding, PCR-RFLP, etc.). This review may be useful to specialists who are in search of an effective system for spider mite species identification as well as when developing new test systems relevant to specific plant crops or a specific region The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2023-06 /pmc/articles/PMC10244583/ /pubmed/37293445 http://dx.doi.org/10.18699/VJGB-23-30 Text en Copyright © AUTHORS https://creativecommons.org/licenses/by/2.5/This work is licensed under a Creative Commons Attribution 4.0 License
spellingShingle Review
Razuvaeva, A.V.
Ulyanova, E.G.
Skolotneva, E.S.
Andreeva, I.V.
Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods
title Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods
title_full Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods
title_fullStr Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods
title_full_unstemmed Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods
title_short Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods
title_sort species identification of spider mites (tetranychidae: tetranychinae): a review of methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244583/
https://www.ncbi.nlm.nih.gov/pubmed/37293445
http://dx.doi.org/10.18699/VJGB-23-30
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