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Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens

Usutu virus (USUV) is a mosquito-borne flavivirus of African origin. Over the past decades, USUV has spread through Europe causing mass die-offs among multiple bird species. The natural transmission cycle of USUV involves Culex spp. mosquitoes as vectors and birds as amplifying hosts. Next to birds...

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Autores principales: van Bree, Joyce W. M., Linthout, Charlotte, van Dijk, Teije, Abbo, Sandra R., Fros, Jelke J., Koenraadt, Constantianus J. M., Pijlman, Gorben P., Wang, Haidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244747/
https://www.ncbi.nlm.nih.gov/pubmed/37293213
http://dx.doi.org/10.3389/fmicb.2023.1195621
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author van Bree, Joyce W. M.
Linthout, Charlotte
van Dijk, Teije
Abbo, Sandra R.
Fros, Jelke J.
Koenraadt, Constantianus J. M.
Pijlman, Gorben P.
Wang, Haidong
author_facet van Bree, Joyce W. M.
Linthout, Charlotte
van Dijk, Teije
Abbo, Sandra R.
Fros, Jelke J.
Koenraadt, Constantianus J. M.
Pijlman, Gorben P.
Wang, Haidong
author_sort van Bree, Joyce W. M.
collection PubMed
description Usutu virus (USUV) is a mosquito-borne flavivirus of African origin. Over the past decades, USUV has spread through Europe causing mass die-offs among multiple bird species. The natural transmission cycle of USUV involves Culex spp. mosquitoes as vectors and birds as amplifying hosts. Next to birds and mosquitoes, USUV has also been isolated from multiple mammalian species, including humans, which are considered dead-end hosts. USUV isolates are phylogenetically classified into an African and European branch, subdivided into eight genetic lineages (Africa 1, 2, and 3 and Europe 1, 2, 3, 4, and 5 lineages). Currently, multiple African and European lineages are co-circulating in Europe. Despite increased knowledge of the epidemiology and pathogenicity of the different lineages, the effects of co-infection and transmission efficacy of the co-circulating USUV strains remain unclear. In this study, we report a comparative study between two USUV isolates as follows: a Dutch isolate (USUV-NL, Africa lineage 3) and an Italian isolate (USUV-IT, Europe lineage 2). Upon co-infection, USUV-NL was consistently outcompeted by USUV-IT in mosquito, mammalian, and avian cell lines. In mosquito cells, the fitness advantage of USUV-IT was most prominently observed in comparison to the mammalian or avian cell lines. When Culex pipiens mosquitoes were orally infected with the different isolates, no overall differences in vector competence for USUV-IT and USUV-NL were observed. However, during the in vivo co-infection assay, it was observed that USUV-NL infectivity and transmission were negatively affected by USUV-IT but not vice versa.
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spelling pubmed-102447472023-06-08 Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens van Bree, Joyce W. M. Linthout, Charlotte van Dijk, Teije Abbo, Sandra R. Fros, Jelke J. Koenraadt, Constantianus J. M. Pijlman, Gorben P. Wang, Haidong Front Microbiol Microbiology Usutu virus (USUV) is a mosquito-borne flavivirus of African origin. Over the past decades, USUV has spread through Europe causing mass die-offs among multiple bird species. The natural transmission cycle of USUV involves Culex spp. mosquitoes as vectors and birds as amplifying hosts. Next to birds and mosquitoes, USUV has also been isolated from multiple mammalian species, including humans, which are considered dead-end hosts. USUV isolates are phylogenetically classified into an African and European branch, subdivided into eight genetic lineages (Africa 1, 2, and 3 and Europe 1, 2, 3, 4, and 5 lineages). Currently, multiple African and European lineages are co-circulating in Europe. Despite increased knowledge of the epidemiology and pathogenicity of the different lineages, the effects of co-infection and transmission efficacy of the co-circulating USUV strains remain unclear. In this study, we report a comparative study between two USUV isolates as follows: a Dutch isolate (USUV-NL, Africa lineage 3) and an Italian isolate (USUV-IT, Europe lineage 2). Upon co-infection, USUV-NL was consistently outcompeted by USUV-IT in mosquito, mammalian, and avian cell lines. In mosquito cells, the fitness advantage of USUV-IT was most prominently observed in comparison to the mammalian or avian cell lines. When Culex pipiens mosquitoes were orally infected with the different isolates, no overall differences in vector competence for USUV-IT and USUV-NL were observed. However, during the in vivo co-infection assay, it was observed that USUV-NL infectivity and transmission were negatively affected by USUV-IT but not vice versa. Frontiers Media S.A. 2023-05-24 /pmc/articles/PMC10244747/ /pubmed/37293213 http://dx.doi.org/10.3389/fmicb.2023.1195621 Text en Copyright © 2023 van Bree, Linthout, van Dijk, Abbo, Fros, Koenraadt, Pijlman and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
van Bree, Joyce W. M.
Linthout, Charlotte
van Dijk, Teije
Abbo, Sandra R.
Fros, Jelke J.
Koenraadt, Constantianus J. M.
Pijlman, Gorben P.
Wang, Haidong
Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens
title Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens
title_full Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens
title_fullStr Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens
title_full_unstemmed Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens
title_short Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens
title_sort competition between two usutu virus isolates in cell culture and in the common house mosquito culex pipiens
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244747/
https://www.ncbi.nlm.nih.gov/pubmed/37293213
http://dx.doi.org/10.3389/fmicb.2023.1195621
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