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Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities

INTRODUCTION: Semen quality is decreasing worldwide, leading to increased male infertility. This study analyzed the microbiota of the gut, semen, and urine in individuals with semen abnormalities to identify potential probiotics and pathogenic bacteria that affect semen parameters and help develop n...

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Autores principales: Cao, Tingshuai, Wang, Shangren, Pan, Yang, Guo, Feng, Wu, Bin, Zhang, Yingchun, Wang, Yujie, Tian, Jiaqing, Xing, Qingfei, Liu, Xiaoqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244769/
https://www.ncbi.nlm.nih.gov/pubmed/37293215
http://dx.doi.org/10.3389/fmicb.2023.1182320
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author Cao, Tingshuai
Wang, Shangren
Pan, Yang
Guo, Feng
Wu, Bin
Zhang, Yingchun
Wang, Yujie
Tian, Jiaqing
Xing, Qingfei
Liu, Xiaoqiang
author_facet Cao, Tingshuai
Wang, Shangren
Pan, Yang
Guo, Feng
Wu, Bin
Zhang, Yingchun
Wang, Yujie
Tian, Jiaqing
Xing, Qingfei
Liu, Xiaoqiang
author_sort Cao, Tingshuai
collection PubMed
description INTRODUCTION: Semen quality is decreasing worldwide, leading to increased male infertility. This study analyzed the microbiota of the gut, semen, and urine in individuals with semen abnormalities to identify potential probiotics and pathogenic bacteria that affect semen parameters and help develop new methods for the diagnosis and treatment of patients with semen abnormalities. METHODS: We recruited 12 individuals with normal semen parameters (control group), 12 with asthenospermia but no semen hyperviscosity (Group_1), 6 with oligospermia (Group_2), 9 with severe oligospermia or azoospermia (Group_3), and 14 with semen hyperviscosity only (Group_4). The semen, gut, and urine microbiota were examined by analyzing the 16S ribosomal RNA gene sequence using next-generation sequencing. RESULTS: The gut microbes were clustered into the highest number of operational taxonomic units, followed by urine and semen. Furthermore, the α-diversity of gut microbes was highest and significantly different from that of urine and semen microbiota. The microbiota of the gut, urine, and semen were all significantly different from each other in terms of β-diversity. The gut abundance of Collinsella was significantly reduced in groups 1, 3, and 4. Furthermore, the gut abundance of Bifidobacterium and Blautia was significantly decreased in Group_1, while that of Bacteroides was significantly increased in Group_3. The abundance of Staphylococcus was significantly increased in the semen of groups 1 and 4. Finally, Lactobacillus abundance was significantly reduced in the urine of groups 2 and 4. DISCUSSION: This study comprehensively describes the differences in intestinal and genitourinary tract microbiota between healthy individuals and those with abnormal semen parameters. Furthermore, our study identified Collinsella, Bifidobacterium, Blautia, and Lactobacillus as potential probiotics. Finally, the study identified Bacteroides in the gut and Staphylococcus in semen as potential pathogenic bacteria. Our study lays the foundation of a new approach to the diagnosis and treatment of male infertility.
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spelling pubmed-102447692023-06-08 Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities Cao, Tingshuai Wang, Shangren Pan, Yang Guo, Feng Wu, Bin Zhang, Yingchun Wang, Yujie Tian, Jiaqing Xing, Qingfei Liu, Xiaoqiang Front Microbiol Microbiology INTRODUCTION: Semen quality is decreasing worldwide, leading to increased male infertility. This study analyzed the microbiota of the gut, semen, and urine in individuals with semen abnormalities to identify potential probiotics and pathogenic bacteria that affect semen parameters and help develop new methods for the diagnosis and treatment of patients with semen abnormalities. METHODS: We recruited 12 individuals with normal semen parameters (control group), 12 with asthenospermia but no semen hyperviscosity (Group_1), 6 with oligospermia (Group_2), 9 with severe oligospermia or azoospermia (Group_3), and 14 with semen hyperviscosity only (Group_4). The semen, gut, and urine microbiota were examined by analyzing the 16S ribosomal RNA gene sequence using next-generation sequencing. RESULTS: The gut microbes were clustered into the highest number of operational taxonomic units, followed by urine and semen. Furthermore, the α-diversity of gut microbes was highest and significantly different from that of urine and semen microbiota. The microbiota of the gut, urine, and semen were all significantly different from each other in terms of β-diversity. The gut abundance of Collinsella was significantly reduced in groups 1, 3, and 4. Furthermore, the gut abundance of Bifidobacterium and Blautia was significantly decreased in Group_1, while that of Bacteroides was significantly increased in Group_3. The abundance of Staphylococcus was significantly increased in the semen of groups 1 and 4. Finally, Lactobacillus abundance was significantly reduced in the urine of groups 2 and 4. DISCUSSION: This study comprehensively describes the differences in intestinal and genitourinary tract microbiota between healthy individuals and those with abnormal semen parameters. Furthermore, our study identified Collinsella, Bifidobacterium, Blautia, and Lactobacillus as potential probiotics. Finally, the study identified Bacteroides in the gut and Staphylococcus in semen as potential pathogenic bacteria. Our study lays the foundation of a new approach to the diagnosis and treatment of male infertility. Frontiers Media S.A. 2023-05-24 /pmc/articles/PMC10244769/ /pubmed/37293215 http://dx.doi.org/10.3389/fmicb.2023.1182320 Text en Copyright © 2023 Cao, Wang, Pan, Guo, Wu, Zhang, Wang, Tian, Xing and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Cao, Tingshuai
Wang, Shangren
Pan, Yang
Guo, Feng
Wu, Bin
Zhang, Yingchun
Wang, Yujie
Tian, Jiaqing
Xing, Qingfei
Liu, Xiaoqiang
Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
title Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
title_full Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
title_fullStr Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
title_full_unstemmed Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
title_short Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
title_sort characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10244769/
https://www.ncbi.nlm.nih.gov/pubmed/37293215
http://dx.doi.org/10.3389/fmicb.2023.1182320
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