Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells

Proximity ligation assays (PLA) use specific antibodies to detect endogenous protein-protein interactions. PLA is a highly useful biochemical technique that allows two proteins within close proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominenc...

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Detalles Bibliográficos
Autores principales: Stephens, Dominique C., Crabtree, Amber, Beasley, Heather K., Garza-Lopez, Edgar, Neikirk, Kit, Mungai, Margaret, Vang, Larry, Vue, Zer, Vue, Neng, Marshall, Andrea G., Turner, Kyrin, Shao, Jianqiang, Murray, Sandra, Gaddy, Jennifer A., Wanjalla, Celestine, Davis, Jamaine, Damo, Steven, Hinton, Antentor O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245739/
https://www.ncbi.nlm.nih.gov/pubmed/37292700
http://dx.doi.org/10.1101/2023.05.20.541599
Descripción
Sumario:Proximity ligation assays (PLA) use specific antibodies to detect endogenous protein-protein interactions. PLA is a highly useful biochemical technique that allows two proteins within close proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominence, the use of PLA in mouse skeletal muscle (SkM) is novel. In this article, we discuss how the PLA method can be used in SkM to study the protein-protein interactions within mitochondria-endoplasmic reticulum contact sites (MERCs).

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