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Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells

Proximity ligation assays (PLA) use specific antibodies to detect endogenous protein-protein interactions. PLA is a highly useful biochemical technique that allows two proteins within close proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominenc...

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Detalles Bibliográficos
Autores principales: Stephens, Dominique C., Crabtree, Amber, Beasley, Heather K., Garza-Lopez, Edgar, Neikirk, Kit, Mungai, Margaret, Vang, Larry, Vue, Zer, Vue, Neng, Marshall, Andrea G., Turner, Kyrin, Shao, Jianqiang, Murray, Sandra, Gaddy, Jennifer A., Wanjalla, Celestine, Davis, Jamaine, Damo, Steven, Hinton, Antentor O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245739/
https://www.ncbi.nlm.nih.gov/pubmed/37292700
http://dx.doi.org/10.1101/2023.05.20.541599