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High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes

Vaginal mucosa-resident anti-viral effector memory B- and T cells appeared to play a crucial role in protection against genital herpes. However, how to mobilize such protective immune cells into the vaginal tissue close to infected epithelial cells remains to be determined. In the present study, we...

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Autores principales: Dhanushkodi, Nisha Rajeswari, Prakash, Swayam, Quadiri, Afshana, Zayou, Latifa, Singer, Mahmoud, Takashi, Nakayama, Vahed, Hawa, BenMohamed, Lbachir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245907/
https://www.ncbi.nlm.nih.gov/pubmed/37292784
http://dx.doi.org/10.1101/2023.05.23.542021
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author Dhanushkodi, Nisha Rajeswari
Prakash, Swayam
Quadiri, Afshana
Zayou, Latifa
Singer, Mahmoud
Takashi, Nakayama
Vahed, Hawa
BenMohamed, Lbachir
author_facet Dhanushkodi, Nisha Rajeswari
Prakash, Swayam
Quadiri, Afshana
Zayou, Latifa
Singer, Mahmoud
Takashi, Nakayama
Vahed, Hawa
BenMohamed, Lbachir
author_sort Dhanushkodi, Nisha Rajeswari
collection PubMed
description Vaginal mucosa-resident anti-viral effector memory B- and T cells appeared to play a crucial role in protection against genital herpes. However, how to mobilize such protective immune cells into the vaginal tissue close to infected epithelial cells remains to be determined. In the present study, we investigate whether and how, CCL28, a major mucosal-associated chemokine, mobilizes effector memory B- and T cells in leading to protecting mucosal surfaces from herpes infection and disease. The CCL28 is a chemoattractant for the CCR10 receptor-expressing immune cells and is produced homeostatically in the human vaginal mucosa (VM). We found the presence of significant frequencies of HSV-specific memory CCR10(+)CD44(+)CD8(+) T cells, expressing high levels of CCR10 receptor, in herpes-infected asymptomatic (ASYMP) women compared to symptomatic (SYMP) women. A significant amount of the CCL28 chemokine (a ligand of CCR10), was detected in the VM of herpes-infected ASYMP B6 mice, associated with the mobilization of high frequencies of HSV-specific effector memory CCR10(+)CD44(+) CD62L(−) CD8(+) T(EM) cells and memory CCR10(+)B220(+)CD27(+) B cells in the VM of HSV-infected asymptomatic mice. In contrast, compared to wild-type (WT) B6 mice, the CCL28 knockout (CCL28((−/−))) mice: (i) Appeared more susceptible to intravaginal infection and re-infection with HSV-2; (ii) Exhibited a significant decrease in the frequencies of HSV-specific effector memory CCR10(+)CD44(+) CD62L(−) CD8(+) T(EM) cells and of memory CD27(+)B220(+) B cells in the infected VM. The results imply a critical role of the CCL28/CCR10 chemokine axis in the mobilization of anti-viral memory B and T cells within the VM to protect against genital herpes infection and disease.
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spelling pubmed-102459072023-06-08 High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes Dhanushkodi, Nisha Rajeswari Prakash, Swayam Quadiri, Afshana Zayou, Latifa Singer, Mahmoud Takashi, Nakayama Vahed, Hawa BenMohamed, Lbachir bioRxiv Article Vaginal mucosa-resident anti-viral effector memory B- and T cells appeared to play a crucial role in protection against genital herpes. However, how to mobilize such protective immune cells into the vaginal tissue close to infected epithelial cells remains to be determined. In the present study, we investigate whether and how, CCL28, a major mucosal-associated chemokine, mobilizes effector memory B- and T cells in leading to protecting mucosal surfaces from herpes infection and disease. The CCL28 is a chemoattractant for the CCR10 receptor-expressing immune cells and is produced homeostatically in the human vaginal mucosa (VM). We found the presence of significant frequencies of HSV-specific memory CCR10(+)CD44(+)CD8(+) T cells, expressing high levels of CCR10 receptor, in herpes-infected asymptomatic (ASYMP) women compared to symptomatic (SYMP) women. A significant amount of the CCL28 chemokine (a ligand of CCR10), was detected in the VM of herpes-infected ASYMP B6 mice, associated with the mobilization of high frequencies of HSV-specific effector memory CCR10(+)CD44(+) CD62L(−) CD8(+) T(EM) cells and memory CCR10(+)B220(+)CD27(+) B cells in the VM of HSV-infected asymptomatic mice. In contrast, compared to wild-type (WT) B6 mice, the CCL28 knockout (CCL28((−/−))) mice: (i) Appeared more susceptible to intravaginal infection and re-infection with HSV-2; (ii) Exhibited a significant decrease in the frequencies of HSV-specific effector memory CCR10(+)CD44(+) CD62L(−) CD8(+) T(EM) cells and of memory CD27(+)B220(+) B cells in the infected VM. The results imply a critical role of the CCL28/CCR10 chemokine axis in the mobilization of anti-viral memory B and T cells within the VM to protect against genital herpes infection and disease. Cold Spring Harbor Laboratory 2023-05-24 /pmc/articles/PMC10245907/ /pubmed/37292784 http://dx.doi.org/10.1101/2023.05.23.542021 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Dhanushkodi, Nisha Rajeswari
Prakash, Swayam
Quadiri, Afshana
Zayou, Latifa
Singer, Mahmoud
Takashi, Nakayama
Vahed, Hawa
BenMohamed, Lbachir
High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
title High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
title_full High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
title_fullStr High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
title_full_unstemmed High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
title_short High Frequencies of Antiviral Effector Memory T(EM) Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes
title_sort high frequencies of antiviral effector memory t(em) cells and memory b cells mobilized into herpes infected vaginal mucosa associated with protection against genital herpes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245907/
https://www.ncbi.nlm.nih.gov/pubmed/37292784
http://dx.doi.org/10.1101/2023.05.23.542021
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