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Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli
Sensory systems allow pathogens to differentiate between different niches and respond to stimuli within them. A major mechanism through which bacteria sense and respond to stimuli in their surroundings is two-component systems (TCSs). TCSs allow for the detection of multiple stimuli to lead to a hig...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245908/ https://www.ncbi.nlm.nih.gov/pubmed/37292752 http://dx.doi.org/10.1101/2023.05.23.541942 |
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author | Brannon, John R. Reasoner, Seth A. Bermudez, Tomas A. Dunigan, Taryn L. Wiebe, Michelle A. Beebout, Connor J. Ross, Tamia Bamidele, Adebisi Hadjifrangiskou, Maria |
author_facet | Brannon, John R. Reasoner, Seth A. Bermudez, Tomas A. Dunigan, Taryn L. Wiebe, Michelle A. Beebout, Connor J. Ross, Tamia Bamidele, Adebisi Hadjifrangiskou, Maria |
author_sort | Brannon, John R. |
collection | PubMed |
description | Sensory systems allow pathogens to differentiate between different niches and respond to stimuli within them. A major mechanism through which bacteria sense and respond to stimuli in their surroundings is two-component systems (TCSs). TCSs allow for the detection of multiple stimuli to lead to a highly controlled and rapid change in gene expression. Here, we provide a comprehensive list of TCSs important for the pathogenesis of uropathogenic Escherichia coli (UPEC). UPEC accounts for >75% of urinary tract infections (UTIs) worldwide. UTIs are most prevalent among people assigned female at birth, with the vagina becoming colonized by UPEC in addition to the gut and the bladder. In the bladder, adherence to the urothelium triggers E. coli invasion of bladder cells and an intracellular pathogenic cascade. Intracellular E. coli are safely hidden from host neutrophils, competition from the microbiota, and antibiotics that kill extracellular E. coli. To survive in these intimately connected, yet physiologically diverse niches E. coli must rapidly coordinate metabolic and virulence systems in response to the distinct stimuli encountered in each environment. We hypothesized that specific TCSs allow UPEC to sense these diverse environments encountered during infection with built-in redundant safeguards. Here, we created a library of isogenic TCS deletion mutants that we leveraged to map distinct TCS contributions to infection. We identify – for the first time – a comprehensive panel of UPEC TCSs that are critical for infection of the genitourinary tract and report that the TCSs mediating colonization of the bladder, kidneys, or vagina are distinct. |
format | Online Article Text |
id | pubmed-10245908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-102459082023-06-08 Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli Brannon, John R. Reasoner, Seth A. Bermudez, Tomas A. Dunigan, Taryn L. Wiebe, Michelle A. Beebout, Connor J. Ross, Tamia Bamidele, Adebisi Hadjifrangiskou, Maria bioRxiv Article Sensory systems allow pathogens to differentiate between different niches and respond to stimuli within them. A major mechanism through which bacteria sense and respond to stimuli in their surroundings is two-component systems (TCSs). TCSs allow for the detection of multiple stimuli to lead to a highly controlled and rapid change in gene expression. Here, we provide a comprehensive list of TCSs important for the pathogenesis of uropathogenic Escherichia coli (UPEC). UPEC accounts for >75% of urinary tract infections (UTIs) worldwide. UTIs are most prevalent among people assigned female at birth, with the vagina becoming colonized by UPEC in addition to the gut and the bladder. In the bladder, adherence to the urothelium triggers E. coli invasion of bladder cells and an intracellular pathogenic cascade. Intracellular E. coli are safely hidden from host neutrophils, competition from the microbiota, and antibiotics that kill extracellular E. coli. To survive in these intimately connected, yet physiologically diverse niches E. coli must rapidly coordinate metabolic and virulence systems in response to the distinct stimuli encountered in each environment. We hypothesized that specific TCSs allow UPEC to sense these diverse environments encountered during infection with built-in redundant safeguards. Here, we created a library of isogenic TCS deletion mutants that we leveraged to map distinct TCS contributions to infection. We identify – for the first time – a comprehensive panel of UPEC TCSs that are critical for infection of the genitourinary tract and report that the TCSs mediating colonization of the bladder, kidneys, or vagina are distinct. Cold Spring Harbor Laboratory 2023-05-23 /pmc/articles/PMC10245908/ /pubmed/37292752 http://dx.doi.org/10.1101/2023.05.23.541942 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Brannon, John R. Reasoner, Seth A. Bermudez, Tomas A. Dunigan, Taryn L. Wiebe, Michelle A. Beebout, Connor J. Ross, Tamia Bamidele, Adebisi Hadjifrangiskou, Maria Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli |
title | Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli |
title_full | Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli |
title_fullStr | Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli |
title_full_unstemmed | Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli |
title_short | Mapping Niche-specific Two-Component System Requirements in Uropathogenic Escherichia coli |
title_sort | mapping niche-specific two-component system requirements in uropathogenic escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245908/ https://www.ncbi.nlm.nih.gov/pubmed/37292752 http://dx.doi.org/10.1101/2023.05.23.541942 |
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