Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation

Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly im...

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Detalles Bibliográficos
Autores principales: Stephens, Dominique C., Mungai, Margaret, Crabtree, Amber, Beasley, Heather K., Garza-Lopez, Edgar, Vang, Larry, Neikirk, Kit, Vue, Zer, Vue, Neng, Marshall, Andrea G., Turner, Kyrin, Shao, Jian-qiang, Sarker, Bishnu, Murray, Sandra, Gaddy, Jennifer A., Davis, Jamaine, Damo, Steven M., Hinton, Antentor O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245929/
https://www.ncbi.nlm.nih.gov/pubmed/37292961
http://dx.doi.org/10.1101/2023.05.20.541600
Descripción
Sumario:Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach.

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