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Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation
Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly im...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245929/ https://www.ncbi.nlm.nih.gov/pubmed/37292961 http://dx.doi.org/10.1101/2023.05.20.541600 |
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| author | Stephens, Dominique C. Mungai, Margaret Crabtree, Amber Beasley, Heather K. Garza-Lopez, Edgar Vang, Larry Neikirk, Kit Vue, Zer Vue, Neng Marshall, Andrea G. Turner, Kyrin Shao, Jian-qiang Sarker, Bishnu Murray, Sandra Gaddy, Jennifer A. Davis, Jamaine Damo, Steven M. Hinton, Antentor O. |
| author_facet | Stephens, Dominique C. Mungai, Margaret Crabtree, Amber Beasley, Heather K. Garza-Lopez, Edgar Vang, Larry Neikirk, Kit Vue, Zer Vue, Neng Marshall, Andrea G. Turner, Kyrin Shao, Jian-qiang Sarker, Bishnu Murray, Sandra Gaddy, Jennifer A. Davis, Jamaine Damo, Steven M. Hinton, Antentor O. |
| author_sort | Stephens, Dominique C. |
| collection | PubMed |
| description | Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach. |
| format | Online Article Text |
| id | pubmed-10245929 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-102459292023-06-08 Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation Stephens, Dominique C. Mungai, Margaret Crabtree, Amber Beasley, Heather K. Garza-Lopez, Edgar Vang, Larry Neikirk, Kit Vue, Zer Vue, Neng Marshall, Andrea G. Turner, Kyrin Shao, Jian-qiang Sarker, Bishnu Murray, Sandra Gaddy, Jennifer A. Davis, Jamaine Damo, Steven M. Hinton, Antentor O. bioRxiv Article Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach. Cold Spring Harbor Laboratory 2023-08-19 /pmc/articles/PMC10245929/ /pubmed/37292961 http://dx.doi.org/10.1101/2023.05.20.541600 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
| spellingShingle | Article Stephens, Dominique C. Mungai, Margaret Crabtree, Amber Beasley, Heather K. Garza-Lopez, Edgar Vang, Larry Neikirk, Kit Vue, Zer Vue, Neng Marshall, Andrea G. Turner, Kyrin Shao, Jian-qiang Sarker, Bishnu Murray, Sandra Gaddy, Jennifer A. Davis, Jamaine Damo, Steven M. Hinton, Antentor O. Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation |
| title | Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation |
| title_full | Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation |
| title_fullStr | Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation |
| title_full_unstemmed | Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation |
| title_short | Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation |
| title_sort | components of isolated skeletal muscle differentiated through antibody validation |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10245929/ https://www.ncbi.nlm.nih.gov/pubmed/37292961 http://dx.doi.org/10.1101/2023.05.20.541600 |
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