Rapid and sensitive detection of genome contamination at scale with FCS-GX

Assembled genome sequences are being generated at an exponential rate. Here we present FCS-GX, part of NCBI’s Foreign Contamination Screen (FCS) tool suite, optimized to identify and remove contaminant sequences in new genomes. FCS-GX screens most genomes in 0.1–10 minutes. Testing FCS-GX on artific...

Descripción completa

Detalles Bibliográficos
Autores principales: Astashyn, Alexander, Tvedte, Eric S., Sweeney, Deacon, Sapojnikov, Victor, Bouk, Nathan, Joukov, Victor, Mozes, Eyal, Strope, Pooja K., Sylla, Pape M., Wagner, Lukas, Bidwell, Shelby L., Clark, Karen, Davis, Emily W., Smith-White, Brian, Hlavina, Wratko, Pruitt, Kim D., Schneider, Valerie A., Murphy, Terence D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246020/
https://www.ncbi.nlm.nih.gov/pubmed/37292984
http://dx.doi.org/10.1101/2023.06.02.543519
Descripción
Sumario:Assembled genome sequences are being generated at an exponential rate. Here we present FCS-GX, part of NCBI’s Foreign Contamination Screen (FCS) tool suite, optimized to identify and remove contaminant sequences in new genomes. FCS-GX screens most genomes in 0.1–10 minutes. Testing FCS-GX on artificially fragmented genomes demonstrates sensitivity >95% for diverse contaminant species and specificity >99.93%. We used FCS-GX to screen 1.6 million GenBank assemblies and identified 36.8 Gbp of contamination (0.16% of total bases), with half from 161 assemblies. We updated assemblies in NCBI RefSeq to reduce detected contamination to 0.01% of bases. FCS-GX is available at https://github.com/ncbi/fcs/.

Ejemplares similares