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Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions

Here we introduce a new endpoint “census population size” to evaluate the epidemiology and control of Plasmodium falciparum infections, where the parasite, rather than the infected human host, is the unit of measurement. To calculate census population size, we rely on a definition of parasite variat...

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Autores principales: Tiedje, Kathryn E., Zhan, Qi, Ruybal-Pésantez, Shazia, Tonkin-Hill, Gerry, He, Qixin, Tan, Mun Hua, Argyropoulos, Dionne C., Deed, Samantha L., Ghansah, Anita, Bangre, Oscar, Oduro, Abraham R., Koram, Kwadwo A., Pascual, Mercedes, Day, Karen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246142/
https://www.ncbi.nlm.nih.gov/pubmed/37292908
http://dx.doi.org/10.1101/2023.05.18.23290210
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author Tiedje, Kathryn E.
Zhan, Qi
Ruybal-Pésantez, Shazia
Tonkin-Hill, Gerry
He, Qixin
Tan, Mun Hua
Argyropoulos, Dionne C.
Deed, Samantha L.
Ghansah, Anita
Bangre, Oscar
Oduro, Abraham R.
Koram, Kwadwo A.
Pascual, Mercedes
Day, Karen P.
author_facet Tiedje, Kathryn E.
Zhan, Qi
Ruybal-Pésantez, Shazia
Tonkin-Hill, Gerry
He, Qixin
Tan, Mun Hua
Argyropoulos, Dionne C.
Deed, Samantha L.
Ghansah, Anita
Bangre, Oscar
Oduro, Abraham R.
Koram, Kwadwo A.
Pascual, Mercedes
Day, Karen P.
author_sort Tiedje, Kathryn E.
collection PubMed
description Here we introduce a new endpoint “census population size” to evaluate the epidemiology and control of Plasmodium falciparum infections, where the parasite, rather than the infected human host, is the unit of measurement. To calculate census population size, we rely on a definition of parasite variation known as multiplicity of infection ([Formula: see text]), based on the hyper-diversity of the [Formula: see text] multigene family. We present a Bayesian approach to estimate [Formula: see text] from sequencing and counting the number of unique DBLα tags (or DBLα types) of [Formula: see text] genes, and derive from it census population size by summation of [Formula: see text] in the human population. We track changes in this parasite population size and structure through sequential malaria interventions by indoor residual spraying (IRS) and seasonal malaria chemoprevention (SMC) from 2012 to 2017 in an area of high-seasonal malaria transmission in northern Ghana. Following IRS, which reduced transmission intensity by > 90% and decreased parasite prevalence by ~40–50%, significant reductions in [Formula: see text] diversity, [Formula: see text] , and population size were observed in ~2,000 humans across all ages. These changes, consistent with the loss of diverse parasite genomes, were short lived and 32-months after IRS was discontinued and SMC was introduced, [Formula: see text] diversity and population size rebounded in all age groups except for the younger children (1–5 years) targeted by SMC. Despite major perturbations from IRS and SMC interventions, the parasite population remained very large and retained the [Formula: see text] population genetic characteristics of a high-transmission system (high [Formula: see text] diversity; low [Formula: see text] repertoire similarity) demonstrating the resilience of P. falciparum to short-term interventions in high-burden countries of sub-Saharan Africa.
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spelling pubmed-102461422023-06-08 Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions Tiedje, Kathryn E. Zhan, Qi Ruybal-Pésantez, Shazia Tonkin-Hill, Gerry He, Qixin Tan, Mun Hua Argyropoulos, Dionne C. Deed, Samantha L. Ghansah, Anita Bangre, Oscar Oduro, Abraham R. Koram, Kwadwo A. Pascual, Mercedes Day, Karen P. medRxiv Article Here we introduce a new endpoint “census population size” to evaluate the epidemiology and control of Plasmodium falciparum infections, where the parasite, rather than the infected human host, is the unit of measurement. To calculate census population size, we rely on a definition of parasite variation known as multiplicity of infection ([Formula: see text]), based on the hyper-diversity of the [Formula: see text] multigene family. We present a Bayesian approach to estimate [Formula: see text] from sequencing and counting the number of unique DBLα tags (or DBLα types) of [Formula: see text] genes, and derive from it census population size by summation of [Formula: see text] in the human population. We track changes in this parasite population size and structure through sequential malaria interventions by indoor residual spraying (IRS) and seasonal malaria chemoprevention (SMC) from 2012 to 2017 in an area of high-seasonal malaria transmission in northern Ghana. Following IRS, which reduced transmission intensity by > 90% and decreased parasite prevalence by ~40–50%, significant reductions in [Formula: see text] diversity, [Formula: see text] , and population size were observed in ~2,000 humans across all ages. These changes, consistent with the loss of diverse parasite genomes, were short lived and 32-months after IRS was discontinued and SMC was introduced, [Formula: see text] diversity and population size rebounded in all age groups except for the younger children (1–5 years) targeted by SMC. Despite major perturbations from IRS and SMC interventions, the parasite population remained very large and retained the [Formula: see text] population genetic characteristics of a high-transmission system (high [Formula: see text] diversity; low [Formula: see text] repertoire similarity) demonstrating the resilience of P. falciparum to short-term interventions in high-burden countries of sub-Saharan Africa. Cold Spring Harbor Laboratory 2023-08-02 /pmc/articles/PMC10246142/ /pubmed/37292908 http://dx.doi.org/10.1101/2023.05.18.23290210 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Tiedje, Kathryn E.
Zhan, Qi
Ruybal-Pésantez, Shazia
Tonkin-Hill, Gerry
He, Qixin
Tan, Mun Hua
Argyropoulos, Dionne C.
Deed, Samantha L.
Ghansah, Anita
Bangre, Oscar
Oduro, Abraham R.
Koram, Kwadwo A.
Pascual, Mercedes
Day, Karen P.
Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions
title Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions
title_full Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions
title_fullStr Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions
title_full_unstemmed Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions
title_short Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions
title_sort measuring changes in plasmodium falciparum census population size in response to sequential malaria control interventions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246142/
https://www.ncbi.nlm.nih.gov/pubmed/37292908
http://dx.doi.org/10.1101/2023.05.18.23290210
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