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Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue

BACKGROUND: The development of nanoscale secondary ion mass spectrometry (NanoSIMS) has revolutionized the study of biological tissues by enabling, e.g., the visualization and quantification of metabolic processes at subcellular length scales. However, the associated sample preparation methods all r...

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Autores principales: Meibom, Anders, Plane, Florent, Cheng, Tian, Grandjean, Gilles, Haldimann, Olivier, Escrig, Stephane, Jensen, Louise, Daraspe, Jean, Mucciolo, Antonio, De Bellis, Damien, Rädecker, Nils, Martin-Olmos, Cristina, Genoud, Christel, Comment, Arnaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246362/
https://www.ncbi.nlm.nih.gov/pubmed/37280616
http://dx.doi.org/10.1186/s12915-023-01623-0
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author Meibom, Anders
Plane, Florent
Cheng, Tian
Grandjean, Gilles
Haldimann, Olivier
Escrig, Stephane
Jensen, Louise
Daraspe, Jean
Mucciolo, Antonio
De Bellis, Damien
Rädecker, Nils
Martin-Olmos, Cristina
Genoud, Christel
Comment, Arnaud
author_facet Meibom, Anders
Plane, Florent
Cheng, Tian
Grandjean, Gilles
Haldimann, Olivier
Escrig, Stephane
Jensen, Louise
Daraspe, Jean
Mucciolo, Antonio
De Bellis, Damien
Rädecker, Nils
Martin-Olmos, Cristina
Genoud, Christel
Comment, Arnaud
author_sort Meibom, Anders
collection PubMed
description BACKGROUND: The development of nanoscale secondary ion mass spectrometry (NanoSIMS) has revolutionized the study of biological tissues by enabling, e.g., the visualization and quantification of metabolic processes at subcellular length scales. However, the associated sample preparation methods all result in some degree of tissue morphology distortion and loss of soluble compounds. To overcome these limitations an entirely cryogenic sample preparation and imaging workflow is required. RESULTS: Here, we report the development of a CryoNanoSIMS instrument that can perform isotope imaging of both positive and negative secondary ions from flat block-face surfaces of vitrified biological tissues with a mass- and image resolution comparable to that of a conventional NanoSIMS. This capability is illustrated with nitrogen isotope as well as trace element mapping of freshwater hydrozoan Green Hydra tissue following uptake of (15)N-enriched ammonium. CONCLUSION: With a cryo-workflow that includes vitrification by high pressure freezing, cryo-planing of the sample surface, and cryo-SEM imaging, the CryoNanoSIMS enables correlative ultrastructure and isotopic or elemental imaging of biological tissues in their most pristine post-mortem state. This opens new horizons in the study of fundamental processes at the tissue- and (sub)cellular level. TEASER: CryoNanoSIMS: subcellular mapping of chemical and isotopic compositions of biological tissues in their most pristine post-mortem state. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-023-01623-0.
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spelling pubmed-102463622023-06-08 Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue Meibom, Anders Plane, Florent Cheng, Tian Grandjean, Gilles Haldimann, Olivier Escrig, Stephane Jensen, Louise Daraspe, Jean Mucciolo, Antonio De Bellis, Damien Rädecker, Nils Martin-Olmos, Cristina Genoud, Christel Comment, Arnaud BMC Biol Methodology Article BACKGROUND: The development of nanoscale secondary ion mass spectrometry (NanoSIMS) has revolutionized the study of biological tissues by enabling, e.g., the visualization and quantification of metabolic processes at subcellular length scales. However, the associated sample preparation methods all result in some degree of tissue morphology distortion and loss of soluble compounds. To overcome these limitations an entirely cryogenic sample preparation and imaging workflow is required. RESULTS: Here, we report the development of a CryoNanoSIMS instrument that can perform isotope imaging of both positive and negative secondary ions from flat block-face surfaces of vitrified biological tissues with a mass- and image resolution comparable to that of a conventional NanoSIMS. This capability is illustrated with nitrogen isotope as well as trace element mapping of freshwater hydrozoan Green Hydra tissue following uptake of (15)N-enriched ammonium. CONCLUSION: With a cryo-workflow that includes vitrification by high pressure freezing, cryo-planing of the sample surface, and cryo-SEM imaging, the CryoNanoSIMS enables correlative ultrastructure and isotopic or elemental imaging of biological tissues in their most pristine post-mortem state. This opens new horizons in the study of fundamental processes at the tissue- and (sub)cellular level. TEASER: CryoNanoSIMS: subcellular mapping of chemical and isotopic compositions of biological tissues in their most pristine post-mortem state. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-023-01623-0. BioMed Central 2023-06-07 /pmc/articles/PMC10246362/ /pubmed/37280616 http://dx.doi.org/10.1186/s12915-023-01623-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Methodology Article
Meibom, Anders
Plane, Florent
Cheng, Tian
Grandjean, Gilles
Haldimann, Olivier
Escrig, Stephane
Jensen, Louise
Daraspe, Jean
Mucciolo, Antonio
De Bellis, Damien
Rädecker, Nils
Martin-Olmos, Cristina
Genoud, Christel
Comment, Arnaud
Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue
title Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue
title_full Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue
title_fullStr Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue
title_full_unstemmed Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue
title_short Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue
title_sort correlated cryo-sem and cryonanosims imaging of biological tissue
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246362/
https://www.ncbi.nlm.nih.gov/pubmed/37280616
http://dx.doi.org/10.1186/s12915-023-01623-0
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