GIL: a python package for designing custom indexing primers

SUMMARY: Generate Indexes for Libraries (GIL) is a software tool for generating primers to be used in the production of multiplexed sequencing libraries. GIL can be customized in numerous ways to meet user specifications, including length, sequencing modality, color balancing, and compatibility with...

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Detalles Bibliográficos
Autores principales: Mateyko, Nicholas, Tariq, Omar, Chen, Xinyi E, Cheney, Will, Salaudeen, Asfar Lathif, Luthra, Ishika, Nikpour, Najmeh, Rafi, Abdul Muntakim, Kamali Deghan, Hadis, Jensen, Cassandra, de Boer, Carl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Applications Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246578/
https://www.ncbi.nlm.nih.gov/pubmed/37208164
http://dx.doi.org/10.1093/bioinformatics/btad328
Descripción
Sumario:SUMMARY: Generate Indexes for Libraries (GIL) is a software tool for generating primers to be used in the production of multiplexed sequencing libraries. GIL can be customized in numerous ways to meet user specifications, including length, sequencing modality, color balancing, and compatibility with existing primers, and produces ordering and demultiplexing-ready outputs. AVAILABILITY AND IMPLEMENTATION: GIL is written in Python and is freely available on GitHub under the MIT license: https://github.com/de-Boer-Lab/GIL and can be accessed as a web-application implemented in Streamlit at https://dbl-gil.streamlitapp.com.

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