Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA

Chemical modifications of CRISPR-Cas nucleases help decrease off-target editing and expand the biomedical applications of CRISPR-based gene manipulation tools. Here, we found that epigenetic modifications of guide RNA, such as m6A and m1A methylation, can effectively inhibit both the cis- and trans-...

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Detalles Bibliográficos
Autores principales: Hu, Zhian, Sun, Ao, Yang, Jinlei, Naz, Gul, Sun, Gongwei, Li, Zhengping, Gogo Liu, Jun-Jie, Zhang, Sichun, Zhang, Xinrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246701/
https://www.ncbi.nlm.nih.gov/pubmed/37293662
http://dx.doi.org/10.1039/d3sc00629h

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246701/
https://www.ncbi.nlm.nih.gov/pubmed/37293662
http://dx.doi.org/10.1039/d3sc00629h

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