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Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study
Platelets are reprogrammed by cancer via a process called education, which favors cancer development. The transcriptional profile of tumor-educated platelets (TEPs) is skewed and therefore practicable for cancer detection. This intercontinental, hospital-based, diagnostic study included 761 treatmen...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246718/ https://www.ncbi.nlm.nih.gov/pubmed/36905391 http://dx.doi.org/10.1093/procel/pwac056 |
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author | Gao, Yue Liu, Chun-Jie Li, Hua-Yi Xiong, Xiao-Ming Li, Gui-Ling In ‘t Veld, Sjors G J G Cai, Guang-Yao Xie, Gui-Yan Zeng, Shao-Qing Wu, Yuan Chi, Jian-Hua Liu, Jia-Hao Zhang, Qiong Jiao, Xiao-Fei Shi, Lin-Li Lu, Wan-Rong Lv, Wei-Guo Yang, Xing-Sheng Piek, Jurgen M J de Kroon, Cornelis D Lok, C A R Supernat, Anna Łapińska-Szumczyk, Sylwia Łojkowska, Anna Żaczek, Anna J Jassem, Jacek Tannous, Bakhos A Sol, Nik Post, Edward Best, Myron G Kong, Bei-Hua Xie, Xing Ma, Ding Wurdinger, Thomas Guo, An-Yuan Gao, Qing-Lei |
author_facet | Gao, Yue Liu, Chun-Jie Li, Hua-Yi Xiong, Xiao-Ming Li, Gui-Ling In ‘t Veld, Sjors G J G Cai, Guang-Yao Xie, Gui-Yan Zeng, Shao-Qing Wu, Yuan Chi, Jian-Hua Liu, Jia-Hao Zhang, Qiong Jiao, Xiao-Fei Shi, Lin-Li Lu, Wan-Rong Lv, Wei-Guo Yang, Xing-Sheng Piek, Jurgen M J de Kroon, Cornelis D Lok, C A R Supernat, Anna Łapińska-Szumczyk, Sylwia Łojkowska, Anna Żaczek, Anna J Jassem, Jacek Tannous, Bakhos A Sol, Nik Post, Edward Best, Myron G Kong, Bei-Hua Xie, Xing Ma, Ding Wurdinger, Thomas Guo, An-Yuan Gao, Qing-Lei |
author_sort | Gao, Yue |
collection | PubMed |
description | Platelets are reprogrammed by cancer via a process called education, which favors cancer development. The transcriptional profile of tumor-educated platelets (TEPs) is skewed and therefore practicable for cancer detection. This intercontinental, hospital-based, diagnostic study included 761 treatment-naïve inpatients with histologically confirmed adnexal masses and 167 healthy controls from nine medical centers (China, n = 3; Netherlands, n = 5; Poland, n = 1) between September 2016 and May 2019. The main outcomes were the performance of TEPs and their combination with CA125 in two Chinese (VC1 and VC2) and the European (VC3) validation cohorts collectively and independently. Exploratory outcome was the value of TEPs in public pan-cancer platelet transcriptome datasets. The AUCs for TEPs in the combined validation cohort, VC1, VC2, and VC3 were 0.918 (95% CI 0.889–0.948), 0.923 (0.855–0.990), 0.918 (0.872–0.963), and 0.887 (0.813–0.960), respectively. Combination of TEPs and CA125 demonstrated an AUC of 0.922 (0.889–0.955) in the combined validation cohort; 0.955 (0.912–0.997) in VC1; 0.939 (0.901–0.977) in VC2; 0.917 (0.824–1.000) in VC3. For subgroup analysis, TEPs exhibited an AUC of 0.858, 0.859, and 0.920 to detect early-stage, borderline, non-epithelial diseases and 0.899 to discriminate ovarian cancer from endometriosis. TEPs had robustness, compatibility, and universality for preoperative diagnosis of ovarian cancer since it withstood validations in populations of different ethnicities, heterogeneous histological subtypes, and early-stage ovarian cancer. However, these observations warrant prospective validations in a larger population before clinical utilities. |
format | Online Article Text |
id | pubmed-10246718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102467182023-06-08 Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study Gao, Yue Liu, Chun-Jie Li, Hua-Yi Xiong, Xiao-Ming Li, Gui-Ling In ‘t Veld, Sjors G J G Cai, Guang-Yao Xie, Gui-Yan Zeng, Shao-Qing Wu, Yuan Chi, Jian-Hua Liu, Jia-Hao Zhang, Qiong Jiao, Xiao-Fei Shi, Lin-Li Lu, Wan-Rong Lv, Wei-Guo Yang, Xing-Sheng Piek, Jurgen M J de Kroon, Cornelis D Lok, C A R Supernat, Anna Łapińska-Szumczyk, Sylwia Łojkowska, Anna Żaczek, Anna J Jassem, Jacek Tannous, Bakhos A Sol, Nik Post, Edward Best, Myron G Kong, Bei-Hua Xie, Xing Ma, Ding Wurdinger, Thomas Guo, An-Yuan Gao, Qing-Lei Protein Cell Research Articles Platelets are reprogrammed by cancer via a process called education, which favors cancer development. The transcriptional profile of tumor-educated platelets (TEPs) is skewed and therefore practicable for cancer detection. This intercontinental, hospital-based, diagnostic study included 761 treatment-naïve inpatients with histologically confirmed adnexal masses and 167 healthy controls from nine medical centers (China, n = 3; Netherlands, n = 5; Poland, n = 1) between September 2016 and May 2019. The main outcomes were the performance of TEPs and their combination with CA125 in two Chinese (VC1 and VC2) and the European (VC3) validation cohorts collectively and independently. Exploratory outcome was the value of TEPs in public pan-cancer platelet transcriptome datasets. The AUCs for TEPs in the combined validation cohort, VC1, VC2, and VC3 were 0.918 (95% CI 0.889–0.948), 0.923 (0.855–0.990), 0.918 (0.872–0.963), and 0.887 (0.813–0.960), respectively. Combination of TEPs and CA125 demonstrated an AUC of 0.922 (0.889–0.955) in the combined validation cohort; 0.955 (0.912–0.997) in VC1; 0.939 (0.901–0.977) in VC2; 0.917 (0.824–1.000) in VC3. For subgroup analysis, TEPs exhibited an AUC of 0.858, 0.859, and 0.920 to detect early-stage, borderline, non-epithelial diseases and 0.899 to discriminate ovarian cancer from endometriosis. TEPs had robustness, compatibility, and universality for preoperative diagnosis of ovarian cancer since it withstood validations in populations of different ethnicities, heterogeneous histological subtypes, and early-stage ovarian cancer. However, these observations warrant prospective validations in a larger population before clinical utilities. Oxford University Press 2022-11-10 /pmc/articles/PMC10246718/ /pubmed/36905391 http://dx.doi.org/10.1093/procel/pwac056 Text en ©The Author(s) 2022. Published by Oxford University Press on behalf of Higher Education Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Gao, Yue Liu, Chun-Jie Li, Hua-Yi Xiong, Xiao-Ming Li, Gui-Ling In ‘t Veld, Sjors G J G Cai, Guang-Yao Xie, Gui-Yan Zeng, Shao-Qing Wu, Yuan Chi, Jian-Hua Liu, Jia-Hao Zhang, Qiong Jiao, Xiao-Fei Shi, Lin-Li Lu, Wan-Rong Lv, Wei-Guo Yang, Xing-Sheng Piek, Jurgen M J de Kroon, Cornelis D Lok, C A R Supernat, Anna Łapińska-Szumczyk, Sylwia Łojkowska, Anna Żaczek, Anna J Jassem, Jacek Tannous, Bakhos A Sol, Nik Post, Edward Best, Myron G Kong, Bei-Hua Xie, Xing Ma, Ding Wurdinger, Thomas Guo, An-Yuan Gao, Qing-Lei Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
title | Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
title_full | Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
title_fullStr | Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
title_full_unstemmed | Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
title_short | Platelet RNA enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
title_sort | platelet rna enables accurate detection of ovarian cancer: an intercontinental, biomarker identification study |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246718/ https://www.ncbi.nlm.nih.gov/pubmed/36905391 http://dx.doi.org/10.1093/procel/pwac056 |
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