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3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System

Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically d...

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Detalles Bibliográficos
Autores principales: Scholaert, Manon, Houmadi, Raissa, Martin, Jeremy, Serhan, Nadine, Tauber, Marie, Braun, Emilie, Basso, Lilian, Merle, Eric, Descargues, Pascal, Viguier, Manuelle, Lesort, Cécile, Chaput, Benoît, Kanitakis, Jean, Jullien, Denis, Livideanu, Cristina Bulai, Lamant, Laurence, Pagès, Emeline, Gaudenzio, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246893/
https://www.ncbi.nlm.nih.gov/pubmed/37285432
http://dx.doi.org/10.1126/sciadv.adf9491
Descripción
Sumario:Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically designed for spatially resolved immune phenotyping of the skin in experimental or clinical samples. On the basis of phenotype attribution matrices coupled to α-shape algorithms, MANTIS projects a representative digital immune landscape while enabling automated detection of major inflammatory clusters and concomitant single-cell data quantification of biomarkers. We observed that severe pathological lesions from systemic lupus erythematosus, Kawasaki syndrome, or COVID-19–associated skin manifestations share common quantitative immune features while displaying a nonrandom distribution of cells with the formation of disease-specific dermal immune structures. Given its accuracy and flexibility, MANTIS is designed to solve the spatial organization of complex immune environments to better apprehend the pathophysiology of skin manifestations.