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3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System

Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically d...

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Autores principales: Scholaert, Manon, Houmadi, Raissa, Martin, Jeremy, Serhan, Nadine, Tauber, Marie, Braun, Emilie, Basso, Lilian, Merle, Eric, Descargues, Pascal, Viguier, Manuelle, Lesort, Cécile, Chaput, Benoît, Kanitakis, Jean, Jullien, Denis, Livideanu, Cristina Bulai, Lamant, Laurence, Pagès, Emeline, Gaudenzio, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246893/
https://www.ncbi.nlm.nih.gov/pubmed/37285432
http://dx.doi.org/10.1126/sciadv.adf9491
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author Scholaert, Manon
Houmadi, Raissa
Martin, Jeremy
Serhan, Nadine
Tauber, Marie
Braun, Emilie
Basso, Lilian
Merle, Eric
Descargues, Pascal
Viguier, Manuelle
Lesort, Cécile
Chaput, Benoît
Kanitakis, Jean
Jullien, Denis
Livideanu, Cristina Bulai
Lamant, Laurence
Pagès, Emeline
Gaudenzio, Nicolas
author_facet Scholaert, Manon
Houmadi, Raissa
Martin, Jeremy
Serhan, Nadine
Tauber, Marie
Braun, Emilie
Basso, Lilian
Merle, Eric
Descargues, Pascal
Viguier, Manuelle
Lesort, Cécile
Chaput, Benoît
Kanitakis, Jean
Jullien, Denis
Livideanu, Cristina Bulai
Lamant, Laurence
Pagès, Emeline
Gaudenzio, Nicolas
author_sort Scholaert, Manon
collection PubMed
description Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically designed for spatially resolved immune phenotyping of the skin in experimental or clinical samples. On the basis of phenotype attribution matrices coupled to α-shape algorithms, MANTIS projects a representative digital immune landscape while enabling automated detection of major inflammatory clusters and concomitant single-cell data quantification of biomarkers. We observed that severe pathological lesions from systemic lupus erythematosus, Kawasaki syndrome, or COVID-19–associated skin manifestations share common quantitative immune features while displaying a nonrandom distribution of cells with the formation of disease-specific dermal immune structures. Given its accuracy and flexibility, MANTIS is designed to solve the spatial organization of complex immune environments to better apprehend the pathophysiology of skin manifestations.
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spelling pubmed-102468932023-06-08 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System Scholaert, Manon Houmadi, Raissa Martin, Jeremy Serhan, Nadine Tauber, Marie Braun, Emilie Basso, Lilian Merle, Eric Descargues, Pascal Viguier, Manuelle Lesort, Cécile Chaput, Benoît Kanitakis, Jean Jullien, Denis Livideanu, Cristina Bulai Lamant, Laurence Pagès, Emeline Gaudenzio, Nicolas Sci Adv Biomedicine and Life Sciences Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. We introduce MANTIS (Multiplex Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically designed for spatially resolved immune phenotyping of the skin in experimental or clinical samples. On the basis of phenotype attribution matrices coupled to α-shape algorithms, MANTIS projects a representative digital immune landscape while enabling automated detection of major inflammatory clusters and concomitant single-cell data quantification of biomarkers. We observed that severe pathological lesions from systemic lupus erythematosus, Kawasaki syndrome, or COVID-19–associated skin manifestations share common quantitative immune features while displaying a nonrandom distribution of cells with the formation of disease-specific dermal immune structures. Given its accuracy and flexibility, MANTIS is designed to solve the spatial organization of complex immune environments to better apprehend the pathophysiology of skin manifestations. American Association for the Advancement of Science 2023-06-07 /pmc/articles/PMC10246893/ /pubmed/37285432 http://dx.doi.org/10.1126/sciadv.adf9491 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Scholaert, Manon
Houmadi, Raissa
Martin, Jeremy
Serhan, Nadine
Tauber, Marie
Braun, Emilie
Basso, Lilian
Merle, Eric
Descargues, Pascal
Viguier, Manuelle
Lesort, Cécile
Chaput, Benoît
Kanitakis, Jean
Jullien, Denis
Livideanu, Cristina Bulai
Lamant, Laurence
Pagès, Emeline
Gaudenzio, Nicolas
3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System
title 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System
title_full 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System
title_fullStr 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System
title_full_unstemmed 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System
title_short 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System
title_sort 3d deconvolution of human skin immune architecture with multiplex annotated tissue imaging system
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10246893/
https://www.ncbi.nlm.nih.gov/pubmed/37285432
http://dx.doi.org/10.1126/sciadv.adf9491
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