Cargando…

Effect of sulforaphane on long-term storage of rabbit semen

In this study, it was aimed to determine the effect of sulforaphane (SFN) on rabbit semen cryopreservation. Semen collected from animals was divided into 5 equal volumes as Control, SFN 5 µM, SFN 10 µM, SFN 25 µM and SFN 50 µM groups. Afterwards, semen analyzes were performed. According to our resul...

Descripción completa

Detalles Bibliográficos
Autores principales: Akarsu, Serkan Ali, Güngör, İbrahim Halil, Cihangiroğlu, Aslıhan Çakır, Acısu, Tutku Can, Koca, Recep Hakkı, Türk, Gaffari, Sönmez, Mustafa, Gür, Seyfettin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Reprodução Animal 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247182/
https://www.ncbi.nlm.nih.gov/pubmed/37293253
http://dx.doi.org/10.1590/1984-3143-AR2023-0001
_version_ 1785055146276814848
author Akarsu, Serkan Ali
Güngör, İbrahim Halil
Cihangiroğlu, Aslıhan Çakır
Acısu, Tutku Can
Koca, Recep Hakkı
Türk, Gaffari
Sönmez, Mustafa
Gür, Seyfettin
author_facet Akarsu, Serkan Ali
Güngör, İbrahim Halil
Cihangiroğlu, Aslıhan Çakır
Acısu, Tutku Can
Koca, Recep Hakkı
Türk, Gaffari
Sönmez, Mustafa
Gür, Seyfettin
author_sort Akarsu, Serkan Ali
collection PubMed
description In this study, it was aimed to determine the effect of sulforaphane (SFN) on rabbit semen cryopreservation. Semen collected from animals was divided into 5 equal volumes as Control, SFN 5 µM, SFN 10 µM, SFN 25 µM and SFN 50 µM groups. Afterwards, semen analyzes were performed. According to our results, there was no statistical difference between the groups at 4°C. However after freezing thawing, the highest total motility, progressive motility and rapid spermatozoa rate was seen in the 10 µM SFN group, while the lowest was observed in the 50 µM SFN group (P<0.05). Static sperm ratio was highest in the 50 µM group, while the lowest was observed in the 10 µM SFN group. When flow cytometry results examined the rate of acrosomal damaged and dead sperm was the lowest in the 10 µM SFN group, a statistical difference was observed between the control group (P<0.05). The highest rate of sperm with high mitochondrial membrane potential was seen in the 5 µM SFN and 10 µM SFN groups. Apoptosis and ROS rates were found to be lower in the experimental groups compared to the control groups (P<0.05). As a result, SFN supplementation at a dose of 10 µM increased the quality of sperm in the freezing and thawing processes of rabbit semen. In conclusion, 10 µM SFN improved the quality of cryopreservation of rabbit semen.
format Online
Article
Text
id pubmed-10247182
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Colégio Brasileiro de Reprodução Animal
record_format MEDLINE/PubMed
spelling pubmed-102471822023-06-08 Effect of sulforaphane on long-term storage of rabbit semen Akarsu, Serkan Ali Güngör, İbrahim Halil Cihangiroğlu, Aslıhan Çakır Acısu, Tutku Can Koca, Recep Hakkı Türk, Gaffari Sönmez, Mustafa Gür, Seyfettin Anim Reprod Original Article In this study, it was aimed to determine the effect of sulforaphane (SFN) on rabbit semen cryopreservation. Semen collected from animals was divided into 5 equal volumes as Control, SFN 5 µM, SFN 10 µM, SFN 25 µM and SFN 50 µM groups. Afterwards, semen analyzes were performed. According to our results, there was no statistical difference between the groups at 4°C. However after freezing thawing, the highest total motility, progressive motility and rapid spermatozoa rate was seen in the 10 µM SFN group, while the lowest was observed in the 50 µM SFN group (P<0.05). Static sperm ratio was highest in the 50 µM group, while the lowest was observed in the 10 µM SFN group. When flow cytometry results examined the rate of acrosomal damaged and dead sperm was the lowest in the 10 µM SFN group, a statistical difference was observed between the control group (P<0.05). The highest rate of sperm with high mitochondrial membrane potential was seen in the 5 µM SFN and 10 µM SFN groups. Apoptosis and ROS rates were found to be lower in the experimental groups compared to the control groups (P<0.05). As a result, SFN supplementation at a dose of 10 µM increased the quality of sperm in the freezing and thawing processes of rabbit semen. In conclusion, 10 µM SFN improved the quality of cryopreservation of rabbit semen. Colégio Brasileiro de Reprodução Animal 2023-05-29 /pmc/articles/PMC10247182/ /pubmed/37293253 http://dx.doi.org/10.1590/1984-3143-AR2023-0001 Text en https://creativecommons.org/licenses/by/4.0/Copyright © The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Akarsu, Serkan Ali
Güngör, İbrahim Halil
Cihangiroğlu, Aslıhan Çakır
Acısu, Tutku Can
Koca, Recep Hakkı
Türk, Gaffari
Sönmez, Mustafa
Gür, Seyfettin
Effect of sulforaphane on long-term storage of rabbit semen
title Effect of sulforaphane on long-term storage of rabbit semen
title_full Effect of sulforaphane on long-term storage of rabbit semen
title_fullStr Effect of sulforaphane on long-term storage of rabbit semen
title_full_unstemmed Effect of sulforaphane on long-term storage of rabbit semen
title_short Effect of sulforaphane on long-term storage of rabbit semen
title_sort effect of sulforaphane on long-term storage of rabbit semen
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247182/
https://www.ncbi.nlm.nih.gov/pubmed/37293253
http://dx.doi.org/10.1590/1984-3143-AR2023-0001
work_keys_str_mv AT akarsuserkanali effectofsulforaphaneonlongtermstorageofrabbitsemen
AT gungoribrahimhalil effectofsulforaphaneonlongtermstorageofrabbitsemen
AT cihangirogluaslıhancakır effectofsulforaphaneonlongtermstorageofrabbitsemen
AT acısututkucan effectofsulforaphaneonlongtermstorageofrabbitsemen
AT kocarecephakkı effectofsulforaphaneonlongtermstorageofrabbitsemen
AT turkgaffari effectofsulforaphaneonlongtermstorageofrabbitsemen
AT sonmezmustafa effectofsulforaphaneonlongtermstorageofrabbitsemen
AT gurseyfettin effectofsulforaphaneonlongtermstorageofrabbitsemen