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Effect of sulforaphane on long-term storage of rabbit semen
In this study, it was aimed to determine the effect of sulforaphane (SFN) on rabbit semen cryopreservation. Semen collected from animals was divided into 5 equal volumes as Control, SFN 5 µM, SFN 10 µM, SFN 25 µM and SFN 50 µM groups. Afterwards, semen analyzes were performed. According to our resul...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247182/ https://www.ncbi.nlm.nih.gov/pubmed/37293253 http://dx.doi.org/10.1590/1984-3143-AR2023-0001 |
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author | Akarsu, Serkan Ali Güngör, İbrahim Halil Cihangiroğlu, Aslıhan Çakır Acısu, Tutku Can Koca, Recep Hakkı Türk, Gaffari Sönmez, Mustafa Gür, Seyfettin |
author_facet | Akarsu, Serkan Ali Güngör, İbrahim Halil Cihangiroğlu, Aslıhan Çakır Acısu, Tutku Can Koca, Recep Hakkı Türk, Gaffari Sönmez, Mustafa Gür, Seyfettin |
author_sort | Akarsu, Serkan Ali |
collection | PubMed |
description | In this study, it was aimed to determine the effect of sulforaphane (SFN) on rabbit semen cryopreservation. Semen collected from animals was divided into 5 equal volumes as Control, SFN 5 µM, SFN 10 µM, SFN 25 µM and SFN 50 µM groups. Afterwards, semen analyzes were performed. According to our results, there was no statistical difference between the groups at 4°C. However after freezing thawing, the highest total motility, progressive motility and rapid spermatozoa rate was seen in the 10 µM SFN group, while the lowest was observed in the 50 µM SFN group (P<0.05). Static sperm ratio was highest in the 50 µM group, while the lowest was observed in the 10 µM SFN group. When flow cytometry results examined the rate of acrosomal damaged and dead sperm was the lowest in the 10 µM SFN group, a statistical difference was observed between the control group (P<0.05). The highest rate of sperm with high mitochondrial membrane potential was seen in the 5 µM SFN and 10 µM SFN groups. Apoptosis and ROS rates were found to be lower in the experimental groups compared to the control groups (P<0.05). As a result, SFN supplementation at a dose of 10 µM increased the quality of sperm in the freezing and thawing processes of rabbit semen. In conclusion, 10 µM SFN improved the quality of cryopreservation of rabbit semen. |
format | Online Article Text |
id | pubmed-10247182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Colégio Brasileiro de Reprodução Animal |
record_format | MEDLINE/PubMed |
spelling | pubmed-102471822023-06-08 Effect of sulforaphane on long-term storage of rabbit semen Akarsu, Serkan Ali Güngör, İbrahim Halil Cihangiroğlu, Aslıhan Çakır Acısu, Tutku Can Koca, Recep Hakkı Türk, Gaffari Sönmez, Mustafa Gür, Seyfettin Anim Reprod Original Article In this study, it was aimed to determine the effect of sulforaphane (SFN) on rabbit semen cryopreservation. Semen collected from animals was divided into 5 equal volumes as Control, SFN 5 µM, SFN 10 µM, SFN 25 µM and SFN 50 µM groups. Afterwards, semen analyzes were performed. According to our results, there was no statistical difference between the groups at 4°C. However after freezing thawing, the highest total motility, progressive motility and rapid spermatozoa rate was seen in the 10 µM SFN group, while the lowest was observed in the 50 µM SFN group (P<0.05). Static sperm ratio was highest in the 50 µM group, while the lowest was observed in the 10 µM SFN group. When flow cytometry results examined the rate of acrosomal damaged and dead sperm was the lowest in the 10 µM SFN group, a statistical difference was observed between the control group (P<0.05). The highest rate of sperm with high mitochondrial membrane potential was seen in the 5 µM SFN and 10 µM SFN groups. Apoptosis and ROS rates were found to be lower in the experimental groups compared to the control groups (P<0.05). As a result, SFN supplementation at a dose of 10 µM increased the quality of sperm in the freezing and thawing processes of rabbit semen. In conclusion, 10 µM SFN improved the quality of cryopreservation of rabbit semen. Colégio Brasileiro de Reprodução Animal 2023-05-29 /pmc/articles/PMC10247182/ /pubmed/37293253 http://dx.doi.org/10.1590/1984-3143-AR2023-0001 Text en https://creativecommons.org/licenses/by/4.0/Copyright © The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Akarsu, Serkan Ali Güngör, İbrahim Halil Cihangiroğlu, Aslıhan Çakır Acısu, Tutku Can Koca, Recep Hakkı Türk, Gaffari Sönmez, Mustafa Gür, Seyfettin Effect of sulforaphane on long-term storage of rabbit semen |
title | Effect of sulforaphane on long-term storage of rabbit semen |
title_full | Effect of sulforaphane on long-term storage of rabbit semen |
title_fullStr | Effect of sulforaphane on long-term storage of rabbit semen |
title_full_unstemmed | Effect of sulforaphane on long-term storage of rabbit semen |
title_short | Effect of sulforaphane on long-term storage of rabbit semen |
title_sort | effect of sulforaphane on long-term storage of rabbit semen |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247182/ https://www.ncbi.nlm.nih.gov/pubmed/37293253 http://dx.doi.org/10.1590/1984-3143-AR2023-0001 |
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