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An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
OBJECTIVE: To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade De Odontologia De Bauru - USP
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247283/ https://www.ncbi.nlm.nih.gov/pubmed/37283330 http://dx.doi.org/10.1590/1678-7757-2023-0006 |
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author | KANTRONG, Nutthapong KUMTAWEE, Jittranut DAMRONGRUNGRUANG, Teerasak PUASIRI, Subin MAKEUDOM, Anupong KRISANAPRAKORNKIT, Suttichai CHAILERTVANITKUL, Pattama |
author_facet | KANTRONG, Nutthapong KUMTAWEE, Jittranut DAMRONGRUNGRUANG, Teerasak PUASIRI, Subin MAKEUDOM, Anupong KRISANAPRAKORNKIT, Suttichai CHAILERTVANITKUL, Pattama |
author_sort | KANTRONG, Nutthapong |
collection | PubMed |
description | OBJECTIVE: To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells. METHODOLOGY: Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. RESULTS: Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. CONCLUSIONS: Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties. |
format | Online Article Text |
id | pubmed-10247283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Faculdade De Odontologia De Bauru - USP |
record_format | MEDLINE/PubMed |
spelling | pubmed-102472832023-06-08 An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells KANTRONG, Nutthapong KUMTAWEE, Jittranut DAMRONGRUNGRUANG, Teerasak PUASIRI, Subin MAKEUDOM, Anupong KRISANAPRAKORNKIT, Suttichai CHAILERTVANITKUL, Pattama J Appl Oral Sci Original Article OBJECTIVE: To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells. METHODOLOGY: Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. RESULTS: Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. CONCLUSIONS: Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties. Faculdade De Odontologia De Bauru - USP 2023-06-05 /pmc/articles/PMC10247283/ /pubmed/37283330 http://dx.doi.org/10.1590/1678-7757-2023-0006 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article KANTRONG, Nutthapong KUMTAWEE, Jittranut DAMRONGRUNGRUANG, Teerasak PUASIRI, Subin MAKEUDOM, Anupong KRISANAPRAKORNKIT, Suttichai CHAILERTVANITKUL, Pattama An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
title | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
title_full | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
title_fullStr | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
title_full_unstemmed | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
title_short | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
title_sort | in vitro anti-inflammatory effect of thai propolis in human dental pulp cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247283/ https://www.ncbi.nlm.nih.gov/pubmed/37283330 http://dx.doi.org/10.1590/1678-7757-2023-0006 |
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