Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway

Argonaute protein (AGO) in association with small RNAs is the core machinery of RNA silencing, an essential mechanism for precise development and defense against pathogens in many organisms. Here, we identified two AGOs in rice anthers, AGO1b and AGO1d, that interact with phased small interfering RN...

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Autores principales: Tamotsu, Hinako, Koizumi, Koji, Briones, Alejandro Villar, Komiya, Reina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247740/
https://www.ncbi.nlm.nih.gov/pubmed/37286636
http://dx.doi.org/10.1038/s41467-023-38881-z
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author Tamotsu, Hinako
Koizumi, Koji
Briones, Alejandro Villar
Komiya, Reina
author_facet Tamotsu, Hinako
Koizumi, Koji
Briones, Alejandro Villar
Komiya, Reina
author_sort Tamotsu, Hinako
collection PubMed
description Argonaute protein (AGO) in association with small RNAs is the core machinery of RNA silencing, an essential mechanism for precise development and defense against pathogens in many organisms. Here, we identified two AGOs in rice anthers, AGO1b and AGO1d, that interact with phased small interfering RNAs (phasiRNAs) derived from numerous long non-coding RNAs. Moreover, 3D-immunoimaging and mutant analysis indicated that rice AGO1b and AGO1d cell type-specifically regulate anther development by acting as mobile carriers of these phasiRNAs from the somatic cell layers to the germ cells in anthers. Our study also highlights a new mode of reproductive RNA silencing via the specific nuclear and cytoplasmic localization of three AGOs, AGO1b, AGO1d, and MEL1, in rice pollen mother cells.
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spelling pubmed-102477402023-06-09 Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway Tamotsu, Hinako Koizumi, Koji Briones, Alejandro Villar Komiya, Reina Nat Commun Article Argonaute protein (AGO) in association with small RNAs is the core machinery of RNA silencing, an essential mechanism for precise development and defense against pathogens in many organisms. Here, we identified two AGOs in rice anthers, AGO1b and AGO1d, that interact with phased small interfering RNAs (phasiRNAs) derived from numerous long non-coding RNAs. Moreover, 3D-immunoimaging and mutant analysis indicated that rice AGO1b and AGO1d cell type-specifically regulate anther development by acting as mobile carriers of these phasiRNAs from the somatic cell layers to the germ cells in anthers. Our study also highlights a new mode of reproductive RNA silencing via the specific nuclear and cytoplasmic localization of three AGOs, AGO1b, AGO1d, and MEL1, in rice pollen mother cells. Nature Publishing Group UK 2023-06-07 /pmc/articles/PMC10247740/ /pubmed/37286636 http://dx.doi.org/10.1038/s41467-023-38881-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tamotsu, Hinako
Koizumi, Koji
Briones, Alejandro Villar
Komiya, Reina
Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
title Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
title_full Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
title_fullStr Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
title_full_unstemmed Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
title_short Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
title_sort spatial distribution of three argonautes regulates the anther phasirna pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247740/
https://www.ncbi.nlm.nih.gov/pubmed/37286636
http://dx.doi.org/10.1038/s41467-023-38881-z
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