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Coherent Stokes Raman scattering microscopy (CSRS)
We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247746/ https://www.ncbi.nlm.nih.gov/pubmed/37286641 http://dx.doi.org/10.1038/s41467-023-38941-4 |
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author | Heuke, Sandro Rigneault, Hervé |
author_facet | Heuke, Sandro Rigneault, Hervé |
author_sort | Heuke, Sandro |
collection | PubMed |
description | We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging of polymer beads, human skin, onion cells, avocado flesh and the wing disc of a drosphila larva are presented. Finally, we explain and demonstrate numerically that CSRS solves a major obstacle of other coherent Raman techniques by sending a significant part (up to 100%) of the CSRS photons into the backward direction under tight focusing conditions. We believe that this discovery will pave the way for numerous technological advances, e.g., in epi-detected coherent Raman multi-focus imaging, real-time laser scanning based spectroscopy or efficient endoscopy. |
format | Online Article Text |
id | pubmed-10247746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102477462023-06-09 Coherent Stokes Raman scattering microscopy (CSRS) Heuke, Sandro Rigneault, Hervé Nat Commun Article We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging of polymer beads, human skin, onion cells, avocado flesh and the wing disc of a drosphila larva are presented. Finally, we explain and demonstrate numerically that CSRS solves a major obstacle of other coherent Raman techniques by sending a significant part (up to 100%) of the CSRS photons into the backward direction under tight focusing conditions. We believe that this discovery will pave the way for numerous technological advances, e.g., in epi-detected coherent Raman multi-focus imaging, real-time laser scanning based spectroscopy or efficient endoscopy. Nature Publishing Group UK 2023-06-07 /pmc/articles/PMC10247746/ /pubmed/37286641 http://dx.doi.org/10.1038/s41467-023-38941-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Heuke, Sandro Rigneault, Hervé Coherent Stokes Raman scattering microscopy (CSRS) |
title | Coherent Stokes Raman scattering microscopy (CSRS) |
title_full | Coherent Stokes Raman scattering microscopy (CSRS) |
title_fullStr | Coherent Stokes Raman scattering microscopy (CSRS) |
title_full_unstemmed | Coherent Stokes Raman scattering microscopy (CSRS) |
title_short | Coherent Stokes Raman scattering microscopy (CSRS) |
title_sort | coherent stokes raman scattering microscopy (csrs) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247746/ https://www.ncbi.nlm.nih.gov/pubmed/37286641 http://dx.doi.org/10.1038/s41467-023-38941-4 |
work_keys_str_mv | AT heukesandro coherentstokesramanscatteringmicroscopycsrs AT rigneaultherve coherentstokesramanscatteringmicroscopycsrs |