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Coherent Stokes Raman scattering microscopy (CSRS)

We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging...

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Detalles Bibliográficos
Autores principales: Heuke, Sandro, Rigneault, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247746/
https://www.ncbi.nlm.nih.gov/pubmed/37286641
http://dx.doi.org/10.1038/s41467-023-38941-4
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author Heuke, Sandro
Rigneault, Hervé
author_facet Heuke, Sandro
Rigneault, Hervé
author_sort Heuke, Sandro
collection PubMed
description We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging of polymer beads, human skin, onion cells, avocado flesh and the wing disc of a drosphila larva are presented. Finally, we explain and demonstrate numerically that CSRS solves a major obstacle of other coherent Raman techniques by sending a significant part (up to 100%) of the CSRS photons into the backward direction under tight focusing conditions. We believe that this discovery will pave the way for numerous technological advances, e.g., in epi-detected coherent Raman multi-focus imaging, real-time laser scanning based spectroscopy or efficient endoscopy.
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spelling pubmed-102477462023-06-09 Coherent Stokes Raman scattering microscopy (CSRS) Heuke, Sandro Rigneault, Hervé Nat Commun Article We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging of polymer beads, human skin, onion cells, avocado flesh and the wing disc of a drosphila larva are presented. Finally, we explain and demonstrate numerically that CSRS solves a major obstacle of other coherent Raman techniques by sending a significant part (up to 100%) of the CSRS photons into the backward direction under tight focusing conditions. We believe that this discovery will pave the way for numerous technological advances, e.g., in epi-detected coherent Raman multi-focus imaging, real-time laser scanning based spectroscopy or efficient endoscopy. Nature Publishing Group UK 2023-06-07 /pmc/articles/PMC10247746/ /pubmed/37286641 http://dx.doi.org/10.1038/s41467-023-38941-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Heuke, Sandro
Rigneault, Hervé
Coherent Stokes Raman scattering microscopy (CSRS)
title Coherent Stokes Raman scattering microscopy (CSRS)
title_full Coherent Stokes Raman scattering microscopy (CSRS)
title_fullStr Coherent Stokes Raman scattering microscopy (CSRS)
title_full_unstemmed Coherent Stokes Raman scattering microscopy (CSRS)
title_short Coherent Stokes Raman scattering microscopy (CSRS)
title_sort coherent stokes raman scattering microscopy (csrs)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247746/
https://www.ncbi.nlm.nih.gov/pubmed/37286641
http://dx.doi.org/10.1038/s41467-023-38941-4
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