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Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults

Phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway regulates glucose and lipid metabolism. We examined the association of PI3K and Akt expression in visceral (VAT) and subcutaneous adipose tissue (SAT) with daily physical activity (PA) in non-diabetic obese and non-obese adults. In this cros...

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Autores principales: Montazeri, Marzieh, Zarkesh, Maryam, Zadeh-Vakili, Azita, Khalili, Davood, Movahedi, Monireh, Khalaj, Alireza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247801/
https://www.ncbi.nlm.nih.gov/pubmed/37286617
http://dx.doi.org/10.1038/s41598-023-36365-0
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author Montazeri, Marzieh
Zarkesh, Maryam
Zadeh-Vakili, Azita
Khalili, Davood
Movahedi, Monireh
Khalaj, Alireza
author_facet Montazeri, Marzieh
Zarkesh, Maryam
Zadeh-Vakili, Azita
Khalili, Davood
Movahedi, Monireh
Khalaj, Alireza
author_sort Montazeri, Marzieh
collection PubMed
description Phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway regulates glucose and lipid metabolism. We examined the association of PI3K and Akt expression in visceral (VAT) and subcutaneous adipose tissue (SAT) with daily physical activity (PA) in non-diabetic obese and non-obese adults. In this cross-sectional study, we included 105 obese (BMI ≥ 30 kg/m(2)) and 71 non-obese (BMI < 30 kg/m(2)) subjects (aged/ ≥ 18 years). PA was measured using a valid and reliable International Physical Activity Questionnaire(IPAQ)-long-form, and the metabolic equivalent of task(MET) was calculated. Real-time PCR was performed to analyze the mRNA relative expression. VAT PI3K expression had a lower level in obese compared to non-obese (P = 0.015), while its expression was higher in active individuals than inactive ones (P = 0.029). SAT PI3K expression was increased in active individuals compared to inactive ones (P = 0.031). There was a rise in VAT Akt expression in the actives compared to the inactive participants (P = 0.037) and in non-obese/active compared to non-obese/inactive individuals (P = 0.026). Obese individuals had a decreased expression level of SAT Akt compared to non-obsesses (P = 0.005). VAT PI3K was directly and significantly associated with PA in obsesses (β = 1.457, P = 0.015). Positive association between PI3K and PA suggests beneficial effects of PA for obese individuals that can be partly described by PI3K/Akt pathway acceleration in adipose tissue.
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spelling pubmed-102478012023-06-09 Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults Montazeri, Marzieh Zarkesh, Maryam Zadeh-Vakili, Azita Khalili, Davood Movahedi, Monireh Khalaj, Alireza Sci Rep Article Phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway regulates glucose and lipid metabolism. We examined the association of PI3K and Akt expression in visceral (VAT) and subcutaneous adipose tissue (SAT) with daily physical activity (PA) in non-diabetic obese and non-obese adults. In this cross-sectional study, we included 105 obese (BMI ≥ 30 kg/m(2)) and 71 non-obese (BMI < 30 kg/m(2)) subjects (aged/ ≥ 18 years). PA was measured using a valid and reliable International Physical Activity Questionnaire(IPAQ)-long-form, and the metabolic equivalent of task(MET) was calculated. Real-time PCR was performed to analyze the mRNA relative expression. VAT PI3K expression had a lower level in obese compared to non-obese (P = 0.015), while its expression was higher in active individuals than inactive ones (P = 0.029). SAT PI3K expression was increased in active individuals compared to inactive ones (P = 0.031). There was a rise in VAT Akt expression in the actives compared to the inactive participants (P = 0.037) and in non-obese/active compared to non-obese/inactive individuals (P = 0.026). Obese individuals had a decreased expression level of SAT Akt compared to non-obsesses (P = 0.005). VAT PI3K was directly and significantly associated with PA in obsesses (β = 1.457, P = 0.015). Positive association between PI3K and PA suggests beneficial effects of PA for obese individuals that can be partly described by PI3K/Akt pathway acceleration in adipose tissue. Nature Publishing Group UK 2023-06-07 /pmc/articles/PMC10247801/ /pubmed/37286617 http://dx.doi.org/10.1038/s41598-023-36365-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Montazeri, Marzieh
Zarkesh, Maryam
Zadeh-Vakili, Azita
Khalili, Davood
Movahedi, Monireh
Khalaj, Alireza
Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults
title Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults
title_full Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults
title_fullStr Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults
title_full_unstemmed Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults
title_short Association of physical activity with increased PI3K and Akt mRNA levels in adipose tissues of obese and non-obese adults
title_sort association of physical activity with increased pi3k and akt mrna levels in adipose tissues of obese and non-obese adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247801/
https://www.ncbi.nlm.nih.gov/pubmed/37286617
http://dx.doi.org/10.1038/s41598-023-36365-0
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