Cargando…

Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study

BACKGROUND: Increasing antibiotic resistance to pathogenic microorganisms (Streptococci) has led scientists around the world to turn to medicinal plants. In this study, the effects of aqueous and alcoholic extracts of Zataria multiflora on the in vitro growth of Streptococcus mutans and Streptococcu...

Descripción completa

Detalles Bibliográficos
Autores principales: Noveiri, Parnian Baradaran, Rezvaninejad, Rayehehossadat, Azarm, Ali, Rezvaninejad, Raziyehsadat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247876/
https://www.ncbi.nlm.nih.gov/pubmed/37304420
_version_ 1785055253198012416
author Noveiri, Parnian Baradaran
Rezvaninejad, Rayehehossadat
Azarm, Ali
Rezvaninejad, Raziyehsadat
author_facet Noveiri, Parnian Baradaran
Rezvaninejad, Rayehehossadat
Azarm, Ali
Rezvaninejad, Raziyehsadat
author_sort Noveiri, Parnian Baradaran
collection PubMed
description BACKGROUND: Increasing antibiotic resistance to pathogenic microorganisms (Streptococci) has led scientists around the world to turn to medicinal plants. In this study, the effects of aqueous and alcoholic extracts of Zataria multiflora on the in vitro growth of Streptococcus mutans and Streptococcus sanguis have been considered and compared with 0.2% chlorhexidine mouthwash. MATERIALS AND METHODS: In this in vitro study, the inhibitory growth zone was accessed by the disc diffusion method after 48 h of incubation at 37 C. To find out the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of treatments, colony counts of cultured bacteria on nutrient agar have been considered at serial dilution at 1/2-1/1024 dilution rates. An independent t-test was used to compare the antibacterial effects of extracts while the level of significance of was considered to be 5% (P < 0.05). RESULTS: The inhibitory growth zones of aqueous and alcoholic extracts on S. mutans were 26.8 mm and 35.8 mm, respectively, whereas growth zones for S. sanguis were considered as 25.8 mm and 33.2 mm, sequentially. Comparisons showed better effects of alcohol compared to aqueous extract (P > 0.05). The MIC and MBC assessments showed the same results (P > 0.05). In all comparisons, the effects of 0.2% chlorhexidine mouthwash were significantly better than both Z. multiflora aqueous and alcoholic extracts (P > 0.05). CONCLUSION: The different solvents may have contributed to the better effects of an alcoholic to aqueous extract of Z. multiflora on the growth of both bacteria. These two extracts could be used for early inhibition of the growth of the planktonic phase, as well as for better oral taste after chlorhexidine applications.
format Online
Article
Text
id pubmed-10247876
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-102478762023-06-09 Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study Noveiri, Parnian Baradaran Rezvaninejad, Rayehehossadat Azarm, Ali Rezvaninejad, Raziyehsadat Dent Res J (Isfahan) Original Article BACKGROUND: Increasing antibiotic resistance to pathogenic microorganisms (Streptococci) has led scientists around the world to turn to medicinal plants. In this study, the effects of aqueous and alcoholic extracts of Zataria multiflora on the in vitro growth of Streptococcus mutans and Streptococcus sanguis have been considered and compared with 0.2% chlorhexidine mouthwash. MATERIALS AND METHODS: In this in vitro study, the inhibitory growth zone was accessed by the disc diffusion method after 48 h of incubation at 37 C. To find out the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of treatments, colony counts of cultured bacteria on nutrient agar have been considered at serial dilution at 1/2-1/1024 dilution rates. An independent t-test was used to compare the antibacterial effects of extracts while the level of significance of was considered to be 5% (P < 0.05). RESULTS: The inhibitory growth zones of aqueous and alcoholic extracts on S. mutans were 26.8 mm and 35.8 mm, respectively, whereas growth zones for S. sanguis were considered as 25.8 mm and 33.2 mm, sequentially. Comparisons showed better effects of alcohol compared to aqueous extract (P > 0.05). The MIC and MBC assessments showed the same results (P > 0.05). In all comparisons, the effects of 0.2% chlorhexidine mouthwash were significantly better than both Z. multiflora aqueous and alcoholic extracts (P > 0.05). CONCLUSION: The different solvents may have contributed to the better effects of an alcoholic to aqueous extract of Z. multiflora on the growth of both bacteria. These two extracts could be used for early inhibition of the growth of the planktonic phase, as well as for better oral taste after chlorhexidine applications. Wolters Kluwer - Medknow 2023-04-26 /pmc/articles/PMC10247876/ /pubmed/37304420 Text en Copyright: © 2023 Dental Research Journal https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Noveiri, Parnian Baradaran
Rezvaninejad, Rayehehossadat
Azarm, Ali
Rezvaninejad, Raziyehsadat
Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study
title Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study
title_full Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study
title_fullStr Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study
title_full_unstemmed Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study
title_short Antibacterial effects of aqueous and alcoholic extracts of Zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: An in vitro study
title_sort antibacterial effects of aqueous and alcoholic extracts of zataria multiflora in comparison with chlorhexidine mouthwash on some pathogenic oral streptococci: an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247876/
https://www.ncbi.nlm.nih.gov/pubmed/37304420
work_keys_str_mv AT noveiriparnianbaradaran antibacterialeffectsofaqueousandalcoholicextractsofzatariamultifloraincomparisonwithchlorhexidinemouthwashonsomepathogenicoralstreptococcianinvitrostudy
AT rezvaninejadrayehehossadat antibacterialeffectsofaqueousandalcoholicextractsofzatariamultifloraincomparisonwithchlorhexidinemouthwashonsomepathogenicoralstreptococcianinvitrostudy
AT azarmali antibacterialeffectsofaqueousandalcoholicextractsofzatariamultifloraincomparisonwithchlorhexidinemouthwashonsomepathogenicoralstreptococcianinvitrostudy
AT rezvaninejadraziyehsadat antibacterialeffectsofaqueousandalcoholicextractsofzatariamultifloraincomparisonwithchlorhexidinemouthwashonsomepathogenicoralstreptococcianinvitrostudy