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Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo

Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 in...

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Autores principales: Li, Zeyu, He, Yuanxiu, Ge, Lijuan, Quan, Ran, Chen, Junzhen, Hu, Yue, Sa, Ruixue, Liu, Jianhua, Ran, Duoliang, Fu, Qiang, Shi, Huijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247970/
https://www.ncbi.nlm.nih.gov/pubmed/37303732
http://dx.doi.org/10.3389/fvets.2023.1163780
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author Li, Zeyu
He, Yuanxiu
Ge, Lijuan
Quan, Ran
Chen, Junzhen
Hu, Yue
Sa, Ruixue
Liu, Jianhua
Ran, Duoliang
Fu, Qiang
Shi, Huijun
author_facet Li, Zeyu
He, Yuanxiu
Ge, Lijuan
Quan, Ran
Chen, Junzhen
Hu, Yue
Sa, Ruixue
Liu, Jianhua
Ran, Duoliang
Fu, Qiang
Shi, Huijun
author_sort Li, Zeyu
collection PubMed
description Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 infection. Quantitative PCR (qPCR), immunoblotting, the Reed-Muench method, and pathological examination were employed to study the ability of BBM to inhibit EHV-1 infection, viral DNA replication, viral protein production, virion secretion, and cytopathogenesis in vitro and in vivo. The in vitro studies revealed that 10 μM BBM effectively suppressed EHV-1 viral entry into cells, viral DNA replication, and virion secretion, while the in vivo studies verified the ability of BBM to suppress EHV-1-induced damage of brain and lung tissues and animal mortality. These findings strongly suggest that BBM could be a serious contender in the therapeutic control of EHV-1 infection of equines.
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spelling pubmed-102479702023-06-09 Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo Li, Zeyu He, Yuanxiu Ge, Lijuan Quan, Ran Chen, Junzhen Hu, Yue Sa, Ruixue Liu, Jianhua Ran, Duoliang Fu, Qiang Shi, Huijun Front Vet Sci Veterinary Science Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 infection. Quantitative PCR (qPCR), immunoblotting, the Reed-Muench method, and pathological examination were employed to study the ability of BBM to inhibit EHV-1 infection, viral DNA replication, viral protein production, virion secretion, and cytopathogenesis in vitro and in vivo. The in vitro studies revealed that 10 μM BBM effectively suppressed EHV-1 viral entry into cells, viral DNA replication, and virion secretion, while the in vivo studies verified the ability of BBM to suppress EHV-1-induced damage of brain and lung tissues and animal mortality. These findings strongly suggest that BBM could be a serious contender in the therapeutic control of EHV-1 infection of equines. Frontiers Media S.A. 2023-05-25 /pmc/articles/PMC10247970/ /pubmed/37303732 http://dx.doi.org/10.3389/fvets.2023.1163780 Text en Copyright © 2023 Li, He, Ge, Quan, Chen, Hu, Sa, Liu, Ran, Fu and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Zeyu
He, Yuanxiu
Ge, Lijuan
Quan, Ran
Chen, Junzhen
Hu, Yue
Sa, Ruixue
Liu, Jianhua
Ran, Duoliang
Fu, Qiang
Shi, Huijun
Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
title Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
title_full Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
title_fullStr Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
title_full_unstemmed Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
title_short Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
title_sort berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10247970/
https://www.ncbi.nlm.nih.gov/pubmed/37303732
http://dx.doi.org/10.3389/fvets.2023.1163780
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