Cargando…
Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment
Seawater immersion wound is inevitably accompanied by bacterial infection. Effective irrigation is critical for bacterial infection prevention and wound healing. In this study, the antimicrobial efficacy of a designed composite irrigation solution against several dominant pathogens in seawater immer...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248133/ https://www.ncbi.nlm.nih.gov/pubmed/37303778 http://dx.doi.org/10.3389/fmicb.2023.1188373 |
_version_ | 1785055306733060096 |
---|---|
author | Wang, Xin Tan, Jie Ni, Shenpeng Zhou, Dengyun Liu, Baolin Fu, Qiang |
author_facet | Wang, Xin Tan, Jie Ni, Shenpeng Zhou, Dengyun Liu, Baolin Fu, Qiang |
author_sort | Wang, Xin |
collection | PubMed |
description | Seawater immersion wound is inevitably accompanied by bacterial infection. Effective irrigation is critical for bacterial infection prevention and wound healing. In this study, the antimicrobial efficacy of a designed composite irrigation solution against several dominant pathogens in seawater immersion wounds was evaluated, and in vivo wound healing assessment was conducted in a rat model. According to the time–kill result, the composite irrigation solution exhibits excellent and rapid bactericidal effect against Vibrio alginolyticus and Vibrio parahaemolyticus within 30 s of treatment while eliminating Candida albicans, Pseudomonas aeruginosa, Escherichia coli, and the mixed microbes after 1 h, 2 h, 6 h, and 12 h of treatment, respectively. Significant bacterial count reduction of Staphylococcus aureus was observed after 5 h treatment. In addition to its skin non-irritating attribute, the in vivo wound healing results further demonstrated that the irrigation solution showed high repair efficiency in the skin defect model inoculated with the mixed microbes. The wound healing rate was significantly higher than that of the control and normal saline groups. It could also effectively reduce the number of viable bacteria on the wound surface. The histological staining indicated that the irrigation solution could reduce inflammatory cells and promote collagen fibers and angiogenesis, thereby promoting wound healing. We believed that the designed composite irrigation solution has great potential for application in the treatment of seawater immersion wounds. |
format | Online Article Text |
id | pubmed-10248133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102481332023-06-09 Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment Wang, Xin Tan, Jie Ni, Shenpeng Zhou, Dengyun Liu, Baolin Fu, Qiang Front Microbiol Microbiology Seawater immersion wound is inevitably accompanied by bacterial infection. Effective irrigation is critical for bacterial infection prevention and wound healing. In this study, the antimicrobial efficacy of a designed composite irrigation solution against several dominant pathogens in seawater immersion wounds was evaluated, and in vivo wound healing assessment was conducted in a rat model. According to the time–kill result, the composite irrigation solution exhibits excellent and rapid bactericidal effect against Vibrio alginolyticus and Vibrio parahaemolyticus within 30 s of treatment while eliminating Candida albicans, Pseudomonas aeruginosa, Escherichia coli, and the mixed microbes after 1 h, 2 h, 6 h, and 12 h of treatment, respectively. Significant bacterial count reduction of Staphylococcus aureus was observed after 5 h treatment. In addition to its skin non-irritating attribute, the in vivo wound healing results further demonstrated that the irrigation solution showed high repair efficiency in the skin defect model inoculated with the mixed microbes. The wound healing rate was significantly higher than that of the control and normal saline groups. It could also effectively reduce the number of viable bacteria on the wound surface. The histological staining indicated that the irrigation solution could reduce inflammatory cells and promote collagen fibers and angiogenesis, thereby promoting wound healing. We believed that the designed composite irrigation solution has great potential for application in the treatment of seawater immersion wounds. Frontiers Media S.A. 2023-05-25 /pmc/articles/PMC10248133/ /pubmed/37303778 http://dx.doi.org/10.3389/fmicb.2023.1188373 Text en Copyright © 2023 Wang, Tan, Ni, Zhou, Liu and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Xin Tan, Jie Ni, Shenpeng Zhou, Dengyun Liu, Baolin Fu, Qiang Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
title | Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
title_full | Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
title_fullStr | Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
title_full_unstemmed | Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
title_short | Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
title_sort | antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248133/ https://www.ncbi.nlm.nih.gov/pubmed/37303778 http://dx.doi.org/10.3389/fmicb.2023.1188373 |
work_keys_str_mv | AT wangxin antimicrobialefficacyofcompositeirrigationsolutionagainstdominantpathogensinseawaterimmersionwoundandinvivowoundhealingassessment AT tanjie antimicrobialefficacyofcompositeirrigationsolutionagainstdominantpathogensinseawaterimmersionwoundandinvivowoundhealingassessment AT nishenpeng antimicrobialefficacyofcompositeirrigationsolutionagainstdominantpathogensinseawaterimmersionwoundandinvivowoundhealingassessment AT zhoudengyun antimicrobialefficacyofcompositeirrigationsolutionagainstdominantpathogensinseawaterimmersionwoundandinvivowoundhealingassessment AT liubaolin antimicrobialefficacyofcompositeirrigationsolutionagainstdominantpathogensinseawaterimmersionwoundandinvivowoundhealingassessment AT fuqiang antimicrobialefficacyofcompositeirrigationsolutionagainstdominantpathogensinseawaterimmersionwoundandinvivowoundhealingassessment |