Cargando…

Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis

Due to high growth rate, outstanding abiotic stress tolerance, and rich value-added substances, Chrysotila roscoffensis, belonging to the phylum of Haptophyta, can be considered as a versatile resource for industrial exploitation of bioactive compounds. However, the application potential of C. rosco...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Jiaojiao, Sun, Yan, Zhang, Lin, Li, Xiaohui, He, Zhichao, Zhou, Chengxu, Han, Jichang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248157/
https://www.ncbi.nlm.nih.gov/pubmed/37304139
http://dx.doi.org/10.3389/fbioe.2023.1218031
_version_ 1785055312543219712
author Liu, Jiaojiao
Sun, Yan
Zhang, Lin
Li, Xiaohui
He, Zhichao
Zhou, Chengxu
Han, Jichang
author_facet Liu, Jiaojiao
Sun, Yan
Zhang, Lin
Li, Xiaohui
He, Zhichao
Zhou, Chengxu
Han, Jichang
author_sort Liu, Jiaojiao
collection PubMed
description Due to high growth rate, outstanding abiotic stress tolerance, and rich value-added substances, Chrysotila roscoffensis, belonging to the phylum of Haptophyta, can be considered as a versatile resource for industrial exploitation of bioactive compounds. However, the application potential of C. roscoffensis has drawn attention until just recently, and the understanding related to the biological properties of this species is still scarce. For example, the sensitivities of C. roscoffensis to antibiotics, which is essential for the verification of heterotrophic capacity and the establishment of efficient genetic manipulation system is still unavailable. Aiming to provide fundamental information for future exploitation, the sensitivities of C. roscoffensis to nine types of antibiotics were tested in this study. The results demonstrated that C. roscoffensis exhibited relatively high resistances to ampicillin, kanamycin, streptomycin, gentamicin, and geneticin, while was sensitive to bleomycin, hygromycin B, paromomycin, and chloramphenicol. Using the former five types of antibiotics, a bacteria removal strategy was established tentatively. Finally, the axenicity of treated C. roscoffensis was confirmed based on a multi-strategy method including solid plate, 16S rDNA amplification, and nuclear acid staining. This report can provide valuable information for the development of optimal selection markers, which are meaningful for more extensive transgenic studies in C. roscoffensis. Moreover, our study also paves the way for the establishment of heterotrophic/mixotrophic cultivation modes of C. roscoffensis.
format Online
Article
Text
id pubmed-10248157
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102481572023-06-09 Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis Liu, Jiaojiao Sun, Yan Zhang, Lin Li, Xiaohui He, Zhichao Zhou, Chengxu Han, Jichang Front Bioeng Biotechnol Bioengineering and Biotechnology Due to high growth rate, outstanding abiotic stress tolerance, and rich value-added substances, Chrysotila roscoffensis, belonging to the phylum of Haptophyta, can be considered as a versatile resource for industrial exploitation of bioactive compounds. However, the application potential of C. roscoffensis has drawn attention until just recently, and the understanding related to the biological properties of this species is still scarce. For example, the sensitivities of C. roscoffensis to antibiotics, which is essential for the verification of heterotrophic capacity and the establishment of efficient genetic manipulation system is still unavailable. Aiming to provide fundamental information for future exploitation, the sensitivities of C. roscoffensis to nine types of antibiotics were tested in this study. The results demonstrated that C. roscoffensis exhibited relatively high resistances to ampicillin, kanamycin, streptomycin, gentamicin, and geneticin, while was sensitive to bleomycin, hygromycin B, paromomycin, and chloramphenicol. Using the former five types of antibiotics, a bacteria removal strategy was established tentatively. Finally, the axenicity of treated C. roscoffensis was confirmed based on a multi-strategy method including solid plate, 16S rDNA amplification, and nuclear acid staining. This report can provide valuable information for the development of optimal selection markers, which are meaningful for more extensive transgenic studies in C. roscoffensis. Moreover, our study also paves the way for the establishment of heterotrophic/mixotrophic cultivation modes of C. roscoffensis. Frontiers Media S.A. 2023-05-25 /pmc/articles/PMC10248157/ /pubmed/37304139 http://dx.doi.org/10.3389/fbioe.2023.1218031 Text en Copyright © 2023 Liu, Sun, Zhang, Li, He, Zhou and Han. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Jiaojiao
Sun, Yan
Zhang, Lin
Li, Xiaohui
He, Zhichao
Zhou, Chengxu
Han, Jichang
Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
title Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
title_full Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
title_fullStr Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
title_full_unstemmed Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
title_short Screening of antibiotics to obtain axenic cell cultures of a marine microalga Chrysotila roscoffensis
title_sort screening of antibiotics to obtain axenic cell cultures of a marine microalga chrysotila roscoffensis
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248157/
https://www.ncbi.nlm.nih.gov/pubmed/37304139
http://dx.doi.org/10.3389/fbioe.2023.1218031
work_keys_str_mv AT liujiaojiao screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis
AT sunyan screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis
AT zhanglin screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis
AT lixiaohui screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis
AT hezhichao screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis
AT zhouchengxu screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis
AT hanjichang screeningofantibioticstoobtainaxeniccellculturesofamarinemicroalgachrysotilaroscoffensis