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Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China
Salmonella is one of the most important foodborne zoonotic pathogens, causing global morbidity and mortality in both humans and animals. Due to the extensive use of antimicrobials in food-producing animals, the antimicrobial resistance of Salmonella has attracted increasing attention globally. There...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248161/ https://www.ncbi.nlm.nih.gov/pubmed/37303807 http://dx.doi.org/10.3389/fmicb.2023.1011719 |
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author | Lai, Jiacui Mu, Hao Zhou, Bingqian He, Jiawei Cheng, Xiangning Gan, Yujie Zhao, Meiyuan Xie, Mengqi Zhang, Yang He, Ying Yang, Yujiao Wang, Jian Wang, Haoju Ding, Honglei |
author_facet | Lai, Jiacui Mu, Hao Zhou, Bingqian He, Jiawei Cheng, Xiangning Gan, Yujie Zhao, Meiyuan Xie, Mengqi Zhang, Yang He, Ying Yang, Yujiao Wang, Jian Wang, Haoju Ding, Honglei |
author_sort | Lai, Jiacui |
collection | PubMed |
description | Salmonella is one of the most important foodborne zoonotic pathogens, causing global morbidity and mortality in both humans and animals. Due to the extensive use of antimicrobials in food-producing animals, the antimicrobial resistance of Salmonella has attracted increasing attention globally. There have been many reports concerning the antimicrobial resistance of Salmonella from food-producing animals, meats and the environment. However, few studies on Salmonella from food-producing animals have been reported in Chongqing municipality, China. The aim of the present study was to determine the prevalence, serovar diversity, sequence types, and antimicrobial resistance of Salmonella isolated from livestock and poultry in Chongqing. Meanwhile, we also want to know the presence of β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes and quinolone resistance-determining region (QRDR) mutations of Salmonella isolates. A total of 129 Salmonella strains were recovered from 2,500 fecal samples at 41 farms from pigs, goats, beef cattle, rabbits, chickens, and ducks. Fourteen serovars were identified, with S. Agona and S. Derby being the dominant serovars. The 129 isolates had high resistance to doxycycline (87.6%), ampicillin (80.6%), tetracycline (79.8%), trimethoprim (77.5%), florfenicol (76.7%) chloramphenicol (72.9%), and trimethoprim-sulfamethoxazole (71.3%), but were susceptible to cefepime. A total of 114 (88.4%) isolates showed multidrug resistant phenotypes. The prevalence of β-lactamase genes in Salmonella isolates was 89.9% (116/129), and among these isolates, 107 (82.9%) harbored bla(TEM), followed by bla(OXA) (26, 20.2%), bla(CTX-M) (8, 6.2%), and bla(CMY) (3, 2.3%). In addition, qnrB, qnrD, qnrS, oqxA, oqxB, and aac(6′)-Ib-cr were detected in 11, 2, 34, 34, 43, and 72 PMQR-producing isolates, respectively. Moreover, QRDR mutations were very common in PMQR-positive Salmonella isolates (97.2%, 70/72) with mutation(s) in parC or combinative mutations in gyrA and parC. More significantly, 32 extended spectrum beta-lactamase (ESBL)-producing isolates were identified, and 62.5% of them were found to harbor one to four PMQR genes. Furthermore, 11 sequence types were identified from the isolates, and most of ESBL-producing isolates were attributed to ST34 (15.6%) and ST40 (62.5%). The coexistence of PMQR genes with β-lactamase genes and the extensive mutations in QRDR present in Salmonella isolates from food-producing animals suggest a potential threat to public health. Reasonable utilization and strict control strategies for antimicrobials in animal husbandry and animal treatment are necessary to reduce the emergence and dissemination of drug-resistant Salmonella isolates. |
format | Online Article Text |
id | pubmed-10248161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102481612023-06-09 Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China Lai, Jiacui Mu, Hao Zhou, Bingqian He, Jiawei Cheng, Xiangning Gan, Yujie Zhao, Meiyuan Xie, Mengqi Zhang, Yang He, Ying Yang, Yujiao Wang, Jian Wang, Haoju Ding, Honglei Front Microbiol Microbiology Salmonella is one of the most important foodborne zoonotic pathogens, causing global morbidity and mortality in both humans and animals. Due to the extensive use of antimicrobials in food-producing animals, the antimicrobial resistance of Salmonella has attracted increasing attention globally. There have been many reports concerning the antimicrobial resistance of Salmonella from food-producing animals, meats and the environment. However, few studies on Salmonella from food-producing animals have been reported in Chongqing municipality, China. The aim of the present study was to determine the prevalence, serovar diversity, sequence types, and antimicrobial resistance of Salmonella isolated from livestock and poultry in Chongqing. Meanwhile, we also want to know the presence of β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes and quinolone resistance-determining region (QRDR) mutations of Salmonella isolates. A total of 129 Salmonella strains were recovered from 2,500 fecal samples at 41 farms from pigs, goats, beef cattle, rabbits, chickens, and ducks. Fourteen serovars were identified, with S. Agona and S. Derby being the dominant serovars. The 129 isolates had high resistance to doxycycline (87.6%), ampicillin (80.6%), tetracycline (79.8%), trimethoprim (77.5%), florfenicol (76.7%) chloramphenicol (72.9%), and trimethoprim-sulfamethoxazole (71.3%), but were susceptible to cefepime. A total of 114 (88.4%) isolates showed multidrug resistant phenotypes. The prevalence of β-lactamase genes in Salmonella isolates was 89.9% (116/129), and among these isolates, 107 (82.9%) harbored bla(TEM), followed by bla(OXA) (26, 20.2%), bla(CTX-M) (8, 6.2%), and bla(CMY) (3, 2.3%). In addition, qnrB, qnrD, qnrS, oqxA, oqxB, and aac(6′)-Ib-cr were detected in 11, 2, 34, 34, 43, and 72 PMQR-producing isolates, respectively. Moreover, QRDR mutations were very common in PMQR-positive Salmonella isolates (97.2%, 70/72) with mutation(s) in parC or combinative mutations in gyrA and parC. More significantly, 32 extended spectrum beta-lactamase (ESBL)-producing isolates were identified, and 62.5% of them were found to harbor one to four PMQR genes. Furthermore, 11 sequence types were identified from the isolates, and most of ESBL-producing isolates were attributed to ST34 (15.6%) and ST40 (62.5%). The coexistence of PMQR genes with β-lactamase genes and the extensive mutations in QRDR present in Salmonella isolates from food-producing animals suggest a potential threat to public health. Reasonable utilization and strict control strategies for antimicrobials in animal husbandry and animal treatment are necessary to reduce the emergence and dissemination of drug-resistant Salmonella isolates. Frontiers Media S.A. 2023-05-25 /pmc/articles/PMC10248161/ /pubmed/37303807 http://dx.doi.org/10.3389/fmicb.2023.1011719 Text en Copyright © 2023 Lai, Mu, Zhou, He, Cheng, Gan, Zhao, Xie, Zhang, He, Yang, Wang and Ding. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lai, Jiacui Mu, Hao Zhou, Bingqian He, Jiawei Cheng, Xiangning Gan, Yujie Zhao, Meiyuan Xie, Mengqi Zhang, Yang He, Ying Yang, Yujiao Wang, Jian Wang, Haoju Ding, Honglei Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China |
title | Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China |
title_full | Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China |
title_fullStr | Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China |
title_full_unstemmed | Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China |
title_short | Bla(TEM)-positive Salmonella enterica serovars Agona and Derby are prevalent among food-producing animals in Chongqing, China |
title_sort | bla(tem)-positive salmonella enterica serovars agona and derby are prevalent among food-producing animals in chongqing, china |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248161/ https://www.ncbi.nlm.nih.gov/pubmed/37303807 http://dx.doi.org/10.3389/fmicb.2023.1011719 |
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