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Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh

Klebsiella pneumoniae is recognized as an urgent public health threat because of the emergence of difficult-to-treat (DTR) strains and hypervirulent clones, resulting in infections with high morbidity and mortality rates. Despite its prominence, little is known about the genomic epidemiology of K. p...

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Autores principales: Hussain, Arif, Mazumder, Razib, Ahmed, Abdullah, Saima, Umme, Phelan, Jody E., Campino, Susana, Ahmed, Dilruba, Asadulghani, Md, Clark, Taane G., Mondal, Dinesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248448/
https://www.ncbi.nlm.nih.gov/pubmed/37303793
http://dx.doi.org/10.3389/fmicb.2023.1184196
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author Hussain, Arif
Mazumder, Razib
Ahmed, Abdullah
Saima, Umme
Phelan, Jody E.
Campino, Susana
Ahmed, Dilruba
Asadulghani, Md
Clark, Taane G.
Mondal, Dinesh
author_facet Hussain, Arif
Mazumder, Razib
Ahmed, Abdullah
Saima, Umme
Phelan, Jody E.
Campino, Susana
Ahmed, Dilruba
Asadulghani, Md
Clark, Taane G.
Mondal, Dinesh
author_sort Hussain, Arif
collection PubMed
description Klebsiella pneumoniae is recognized as an urgent public health threat because of the emergence of difficult-to-treat (DTR) strains and hypervirulent clones, resulting in infections with high morbidity and mortality rates. Despite its prominence, little is known about the genomic epidemiology of K. pneumoniae in resource-limited settings like Bangladesh. We sequenced genomes of 32 K. pneumoniae strains isolated from patient samples at the International Center for Diarrhoeal Disease Research, Bangladesh (icddr,b). Genome sequences were examined for their diversity, population structure, resistome, virulome, MLST, O and K antigens and plasmids. Our results revealed the presence of two K. pneumoniae phylogroups, namely KpI (K. pneumoniae) (97%) and KpII (K. quasipneumoniae) (3%). The genomic characterization revealed that 25% (8/32) of isolates were associated with high-risk multidrug-resistant clones, including ST11, ST14, ST15, ST307, ST231 and ST147. The virulome analysis confirmed the presence of six (19%) hypervirulent K. pneumoniae (hvKp) and 26 (81%) classical K. pneumoniae (cKp) strains. The most common ESBL gene identified was bla(CTX-M-15) (50%). Around 9% (3/32) isolates exhibited a difficult-to-treat phenotype, harboring carbapenem resistance genes (2 strains harbored bla(NDM-5) plus bla(OXA-232), one isolate bla(OXA-181)). The most prevalent O antigen was O1 (56%). The capsular polysaccharides K2, K20, K16 and K62 were enriched in the K. pneumoniae population. This study suggests the circulation of the major international high-risk multidrug-resistant and hypervirulent (hvKp) K. pneumoniae clones in Dhaka, Bangladesh. These findings warrant immediate appropriate interventions, which would otherwise lead to a high burden of untreatable life-threatening infections locally.
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spelling pubmed-102484482023-06-09 Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh Hussain, Arif Mazumder, Razib Ahmed, Abdullah Saima, Umme Phelan, Jody E. Campino, Susana Ahmed, Dilruba Asadulghani, Md Clark, Taane G. Mondal, Dinesh Front Microbiol Microbiology Klebsiella pneumoniae is recognized as an urgent public health threat because of the emergence of difficult-to-treat (DTR) strains and hypervirulent clones, resulting in infections with high morbidity and mortality rates. Despite its prominence, little is known about the genomic epidemiology of K. pneumoniae in resource-limited settings like Bangladesh. We sequenced genomes of 32 K. pneumoniae strains isolated from patient samples at the International Center for Diarrhoeal Disease Research, Bangladesh (icddr,b). Genome sequences were examined for their diversity, population structure, resistome, virulome, MLST, O and K antigens and plasmids. Our results revealed the presence of two K. pneumoniae phylogroups, namely KpI (K. pneumoniae) (97%) and KpII (K. quasipneumoniae) (3%). The genomic characterization revealed that 25% (8/32) of isolates were associated with high-risk multidrug-resistant clones, including ST11, ST14, ST15, ST307, ST231 and ST147. The virulome analysis confirmed the presence of six (19%) hypervirulent K. pneumoniae (hvKp) and 26 (81%) classical K. pneumoniae (cKp) strains. The most common ESBL gene identified was bla(CTX-M-15) (50%). Around 9% (3/32) isolates exhibited a difficult-to-treat phenotype, harboring carbapenem resistance genes (2 strains harbored bla(NDM-5) plus bla(OXA-232), one isolate bla(OXA-181)). The most prevalent O antigen was O1 (56%). The capsular polysaccharides K2, K20, K16 and K62 were enriched in the K. pneumoniae population. This study suggests the circulation of the major international high-risk multidrug-resistant and hypervirulent (hvKp) K. pneumoniae clones in Dhaka, Bangladesh. These findings warrant immediate appropriate interventions, which would otherwise lead to a high burden of untreatable life-threatening infections locally. Frontiers Media S.A. 2023-05-25 /pmc/articles/PMC10248448/ /pubmed/37303793 http://dx.doi.org/10.3389/fmicb.2023.1184196 Text en Copyright © 2023 Hussain, Mazumder, Ahmed, Saima, Phelan, Campino, Ahmed, Asadulghani, Clark and Mondal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hussain, Arif
Mazumder, Razib
Ahmed, Abdullah
Saima, Umme
Phelan, Jody E.
Campino, Susana
Ahmed, Dilruba
Asadulghani, Md
Clark, Taane G.
Mondal, Dinesh
Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh
title Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh
title_full Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh
title_fullStr Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh
title_full_unstemmed Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh
title_short Genome dynamics of high-risk resistant and hypervirulent Klebsiella pneumoniae clones in Dhaka, Bangladesh
title_sort genome dynamics of high-risk resistant and hypervirulent klebsiella pneumoniae clones in dhaka, bangladesh
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248448/
https://www.ncbi.nlm.nih.gov/pubmed/37303793
http://dx.doi.org/10.3389/fmicb.2023.1184196
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