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Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition
It has been proposed that the composition of the culture medium, especially its amino acids, is an important part of getting microspore androgenesis to occur in some plants. However, there have been far fewer studies done on the Solanaceae family. In this study, we studied what happened to eggplant...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10249880/ https://www.ncbi.nlm.nih.gov/pubmed/37289731 http://dx.doi.org/10.1371/journal.pone.0286809 |
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author | Hashemi, Mozhgan Moieni, Ahmad Sabet, Mohammad Sadegh |
author_facet | Hashemi, Mozhgan Moieni, Ahmad Sabet, Mohammad Sadegh |
author_sort | Hashemi, Mozhgan |
collection | PubMed |
description | It has been proposed that the composition of the culture medium, especially its amino acids, is an important part of getting microspore androgenesis to occur in some plants. However, there have been far fewer studies done on the Solanaceae family. In this study, we studied what happened to eggplant microspore culture when we mixed casein hydrolysate (0 and 100 mg L(-1)) with four amino acids: proline (0, 100, 500, and 900 mg L(-1)), glutamine (0 and 800 mg L(-1)), serine (0 and 100 mg L(-1)), and alanine (0 and 100 mg L(-1)). The results showed that a combination of 800 mg L(-1) of glutamine, 100 mg L(-1) of serine, 100 mg L(-1) of casein hydrolysate, and 500 mg L(-1) of proline produced the maximum number of calli per Petri dish (938). Calli had a globular shape and a compact appearance when formed in media containing 500 mg L(-1) of proline (alone or combined with serine, alanine, and/or casein hydrolysate). Most of these structures were observed in a medium with 500 mg L(-1) of proline, 100 mg L(-1) of casein hydrolysate, and 100 mg L(-1) of serine. We also investigated what happened when gum arabic (2400, 2600, 3600, 4600, and 5600 mg L(-1)) was combined with proline (0 and 500 mg L(-1)), casein hydrolysate (0 and 100 mg L(-1)), and glutamine (0, 400, and 800 mg L(-1)). The findings demonstrated the involvement of proline in the increase of calli. Overall, the results give us new information about how amino acids work in eggplant microspore culture and suggest that proline can move this plant’s microspore androgenesis pathway forward. |
format | Online Article Text |
id | pubmed-10249880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102498802023-06-09 Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition Hashemi, Mozhgan Moieni, Ahmad Sabet, Mohammad Sadegh PLoS One Research Article It has been proposed that the composition of the culture medium, especially its amino acids, is an important part of getting microspore androgenesis to occur in some plants. However, there have been far fewer studies done on the Solanaceae family. In this study, we studied what happened to eggplant microspore culture when we mixed casein hydrolysate (0 and 100 mg L(-1)) with four amino acids: proline (0, 100, 500, and 900 mg L(-1)), glutamine (0 and 800 mg L(-1)), serine (0 and 100 mg L(-1)), and alanine (0 and 100 mg L(-1)). The results showed that a combination of 800 mg L(-1) of glutamine, 100 mg L(-1) of serine, 100 mg L(-1) of casein hydrolysate, and 500 mg L(-1) of proline produced the maximum number of calli per Petri dish (938). Calli had a globular shape and a compact appearance when formed in media containing 500 mg L(-1) of proline (alone or combined with serine, alanine, and/or casein hydrolysate). Most of these structures were observed in a medium with 500 mg L(-1) of proline, 100 mg L(-1) of casein hydrolysate, and 100 mg L(-1) of serine. We also investigated what happened when gum arabic (2400, 2600, 3600, 4600, and 5600 mg L(-1)) was combined with proline (0 and 500 mg L(-1)), casein hydrolysate (0 and 100 mg L(-1)), and glutamine (0, 400, and 800 mg L(-1)). The findings demonstrated the involvement of proline in the increase of calli. Overall, the results give us new information about how amino acids work in eggplant microspore culture and suggest that proline can move this plant’s microspore androgenesis pathway forward. Public Library of Science 2023-06-08 /pmc/articles/PMC10249880/ /pubmed/37289731 http://dx.doi.org/10.1371/journal.pone.0286809 Text en © 2023 Hashemi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hashemi, Mozhgan Moieni, Ahmad Sabet, Mohammad Sadegh Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition |
title | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition |
title_full | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition |
title_fullStr | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition |
title_full_unstemmed | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition |
title_short | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition |
title_sort | improving the isolated microspore culture in eggplant (solanum melongena l.) with amino acid nutrition |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10249880/ https://www.ncbi.nlm.nih.gov/pubmed/37289731 http://dx.doi.org/10.1371/journal.pone.0286809 |
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