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Label-free microscopy for virus infections

Microscopy has been essential to elucidate micro- and nano-scale processes in space and time and has provided insights into cell and organismic functions. It is widely employed in cell biology, microbiology, physiology, clinical sciences and virology. While label-dependent microscopy, such as fluore...

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Autores principales: Petkidis, Anthony, Andriasyan, Vardan, Greber, Urs F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250014/
https://www.ncbi.nlm.nih.gov/pubmed/37079744
http://dx.doi.org/10.1093/jmicro/dfad024
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author Petkidis, Anthony
Andriasyan, Vardan
Greber, Urs F
author_facet Petkidis, Anthony
Andriasyan, Vardan
Greber, Urs F
author_sort Petkidis, Anthony
collection PubMed
description Microscopy has been essential to elucidate micro- and nano-scale processes in space and time and has provided insights into cell and organismic functions. It is widely employed in cell biology, microbiology, physiology, clinical sciences and virology. While label-dependent microscopy, such as fluorescence microscopy, provides molecular specificity, it has remained difficult to multiplex in live samples. In contrast, label-free microscopy reports on overall features of the specimen at minimal perturbation. Here, we discuss modalities of label-free imaging at the molecular, cellular and tissue levels, including transmitted light microscopy, quantitative phase imaging, cryogenic electron microscopy or tomography and atomic force microscopy. We highlight how label-free microscopy is used to probe the structural organization and mechanical properties of viruses, including virus particles and infected cells across a wide range of spatial scales. We discuss the working principles of imaging procedures and analyses and showcase how they open new avenues in virology. Finally, we discuss orthogonal approaches that enhance and complement label-free microscopy techniques.
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spelling pubmed-102500142023-06-09 Label-free microscopy for virus infections Petkidis, Anthony Andriasyan, Vardan Greber, Urs F Microscopy (Oxf) Special Feature: Review Microscopy has been essential to elucidate micro- and nano-scale processes in space and time and has provided insights into cell and organismic functions. It is widely employed in cell biology, microbiology, physiology, clinical sciences and virology. While label-dependent microscopy, such as fluorescence microscopy, provides molecular specificity, it has remained difficult to multiplex in live samples. In contrast, label-free microscopy reports on overall features of the specimen at minimal perturbation. Here, we discuss modalities of label-free imaging at the molecular, cellular and tissue levels, including transmitted light microscopy, quantitative phase imaging, cryogenic electron microscopy or tomography and atomic force microscopy. We highlight how label-free microscopy is used to probe the structural organization and mechanical properties of viruses, including virus particles and infected cells across a wide range of spatial scales. We discuss the working principles of imaging procedures and analyses and showcase how they open new avenues in virology. Finally, we discuss orthogonal approaches that enhance and complement label-free microscopy techniques. Oxford University Press 2023-04-20 /pmc/articles/PMC10250014/ /pubmed/37079744 http://dx.doi.org/10.1093/jmicro/dfad024 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Special Feature: Review
Petkidis, Anthony
Andriasyan, Vardan
Greber, Urs F
Label-free microscopy for virus infections
title Label-free microscopy for virus infections
title_full Label-free microscopy for virus infections
title_fullStr Label-free microscopy for virus infections
title_full_unstemmed Label-free microscopy for virus infections
title_short Label-free microscopy for virus infections
title_sort label-free microscopy for virus infections
topic Special Feature: Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250014/
https://www.ncbi.nlm.nih.gov/pubmed/37079744
http://dx.doi.org/10.1093/jmicro/dfad024
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