High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics

Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5–10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications,...

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Autores principales: Song, Kun, Feng, Zhihui, Helmstaedter, Moritz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250191/
https://www.ncbi.nlm.nih.gov/pubmed/37156843
http://dx.doi.org/10.1038/s41592-023-01866-3
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author Song, Kun
Feng, Zhihui
Helmstaedter, Moritz
author_facet Song, Kun
Feng, Zhihui
Helmstaedter, Moritz
author_sort Song, Kun
collection PubMed
description Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5–10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.
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spelling pubmed-102501912023-06-10 High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics Song, Kun Feng, Zhihui Helmstaedter, Moritz Nat Methods Brief Communication Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5–10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level. Nature Publishing Group US 2023-05-08 2023 /pmc/articles/PMC10250191/ /pubmed/37156843 http://dx.doi.org/10.1038/s41592-023-01866-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Song, Kun
Feng, Zhihui
Helmstaedter, Moritz
High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
title High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
title_full High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
title_fullStr High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
title_full_unstemmed High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
title_short High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
title_sort high-contrast en bloc staining of mouse whole-brain and human brain samples for em-based connectomics
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250191/
https://www.ncbi.nlm.nih.gov/pubmed/37156843
http://dx.doi.org/10.1038/s41592-023-01866-3
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