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High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5–10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications,...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250191/ https://www.ncbi.nlm.nih.gov/pubmed/37156843 http://dx.doi.org/10.1038/s41592-023-01866-3 |