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A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo

Targeted in vivo hypermutation accelerates directed evolution of proteins through concurrent DNA diversification and selection. Although systems employing a fusion protein of a nucleobase deaminase and T7 RNA polymerase present gene-specific targeting, their mutational spectra have been limited to e...

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Detalles Bibliográficos
Autores principales:	Seo, Daeje, Koh, Bonghyun, Eom, Ga-eul, Kim, Hye Won, Kim, Seokhee
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250238/ https://www.ncbi.nlm.nih.gov/pubmed/37070179 http://dx.doi.org/10.1093/nar/gkad266

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