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Origin of minicircular mitochondrial genomes in red algae

Eukaryotic organelle genomes are generally of conserved size and gene content within phylogenetic groups. However, significant variation in genome structure may occur. Here, we report that the Stylonematophyceae red algae contain multipartite circular mitochondrial genomes (i.e., minicircles) which...

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Autores principales: Lee, Yongsung, Cho, Chung Hyun, Noh, Chanyoung, Yang, Ji Hyun, Park, Seung In, Lee, Yu Min, West, John A., Bhattacharya, Debashish, Jo, Kyubong, Yoon, Hwan Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250338/
https://www.ncbi.nlm.nih.gov/pubmed/37291154
http://dx.doi.org/10.1038/s41467-023-39084-2
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author Lee, Yongsung
Cho, Chung Hyun
Noh, Chanyoung
Yang, Ji Hyun
Park, Seung In
Lee, Yu Min
West, John A.
Bhattacharya, Debashish
Jo, Kyubong
Yoon, Hwan Su
author_facet Lee, Yongsung
Cho, Chung Hyun
Noh, Chanyoung
Yang, Ji Hyun
Park, Seung In
Lee, Yu Min
West, John A.
Bhattacharya, Debashish
Jo, Kyubong
Yoon, Hwan Su
author_sort Lee, Yongsung
collection PubMed
description Eukaryotic organelle genomes are generally of conserved size and gene content within phylogenetic groups. However, significant variation in genome structure may occur. Here, we report that the Stylonematophyceae red algae contain multipartite circular mitochondrial genomes (i.e., minicircles) which encode one or two genes bounded by a specific cassette and a conserved constant region. These minicircles are visualized using fluorescence microscope and scanning electron microscope, proving the circularity. Mitochondrial gene sets are reduced in these highly divergent mitogenomes. Newly generated chromosome-level nuclear genome assembly of Rhodosorus marinus reveals that most mitochondrial ribosomal subunit genes are transferred to the nuclear genome. Hetero-concatemers that resulted from recombination between minicircles and unique gene inventory that is responsible for mitochondrial genome stability may explain how the transition from typical mitochondrial genome to minicircles occurs. Our results offer inspiration on minicircular organelle genome formation and highlight an extreme case of mitochondrial gene inventory reduction.
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spelling pubmed-102503382023-06-10 Origin of minicircular mitochondrial genomes in red algae Lee, Yongsung Cho, Chung Hyun Noh, Chanyoung Yang, Ji Hyun Park, Seung In Lee, Yu Min West, John A. Bhattacharya, Debashish Jo, Kyubong Yoon, Hwan Su Nat Commun Article Eukaryotic organelle genomes are generally of conserved size and gene content within phylogenetic groups. However, significant variation in genome structure may occur. Here, we report that the Stylonematophyceae red algae contain multipartite circular mitochondrial genomes (i.e., minicircles) which encode one or two genes bounded by a specific cassette and a conserved constant region. These minicircles are visualized using fluorescence microscope and scanning electron microscope, proving the circularity. Mitochondrial gene sets are reduced in these highly divergent mitogenomes. Newly generated chromosome-level nuclear genome assembly of Rhodosorus marinus reveals that most mitochondrial ribosomal subunit genes are transferred to the nuclear genome. Hetero-concatemers that resulted from recombination between minicircles and unique gene inventory that is responsible for mitochondrial genome stability may explain how the transition from typical mitochondrial genome to minicircles occurs. Our results offer inspiration on minicircular organelle genome formation and highlight an extreme case of mitochondrial gene inventory reduction. Nature Publishing Group UK 2023-06-08 /pmc/articles/PMC10250338/ /pubmed/37291154 http://dx.doi.org/10.1038/s41467-023-39084-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lee, Yongsung
Cho, Chung Hyun
Noh, Chanyoung
Yang, Ji Hyun
Park, Seung In
Lee, Yu Min
West, John A.
Bhattacharya, Debashish
Jo, Kyubong
Yoon, Hwan Su
Origin of minicircular mitochondrial genomes in red algae
title Origin of minicircular mitochondrial genomes in red algae
title_full Origin of minicircular mitochondrial genomes in red algae
title_fullStr Origin of minicircular mitochondrial genomes in red algae
title_full_unstemmed Origin of minicircular mitochondrial genomes in red algae
title_short Origin of minicircular mitochondrial genomes in red algae
title_sort origin of minicircular mitochondrial genomes in red algae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250338/
https://www.ncbi.nlm.nih.gov/pubmed/37291154
http://dx.doi.org/10.1038/s41467-023-39084-2
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