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Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have re...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250713/ https://www.ncbi.nlm.nih.gov/pubmed/37303723 http://dx.doi.org/10.3389/fvets.2023.1179836 |
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author | Nagao, Itsuma Ambrosini, Yoko M. |
author_facet | Nagao, Itsuma Ambrosini, Yoko M. |
author_sort | Nagao, Itsuma |
collection | PubMed |
description | The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have recently gained attention in the investigation of human diseases due to their remarkable similarities in physiology and pathophysiology with the human gallbladder. In this study, we have successfully established and characterized bovine gallbladder cholangiocyte organoids (GCOs) that retain key characteristics of the gallbladder in vivo, including stem cell properties and proliferative capacity. Notably, our findings demonstrate that these organoids exhibit specific and functional CFTR activity. We believe that these bovine GCOs represent a valuable tool for studying the physiology and pathophysiology of the gallbladder with human significance. |
format | Online Article Text |
id | pubmed-10250713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102507132023-06-10 Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system Nagao, Itsuma Ambrosini, Yoko M. Front Vet Sci Veterinary Science The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have recently gained attention in the investigation of human diseases due to their remarkable similarities in physiology and pathophysiology with the human gallbladder. In this study, we have successfully established and characterized bovine gallbladder cholangiocyte organoids (GCOs) that retain key characteristics of the gallbladder in vivo, including stem cell properties and proliferative capacity. Notably, our findings demonstrate that these organoids exhibit specific and functional CFTR activity. We believe that these bovine GCOs represent a valuable tool for studying the physiology and pathophysiology of the gallbladder with human significance. Frontiers Media S.A. 2023-05-26 /pmc/articles/PMC10250713/ /pubmed/37303723 http://dx.doi.org/10.3389/fvets.2023.1179836 Text en Copyright © 2023 Nagao and Ambrosini. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Nagao, Itsuma Ambrosini, Yoko M. Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
title | Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
title_full | Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
title_fullStr | Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
title_full_unstemmed | Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
title_short | Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
title_sort | ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250713/ https://www.ncbi.nlm.nih.gov/pubmed/37303723 http://dx.doi.org/10.3389/fvets.2023.1179836 |
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