Cargando…

Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system

The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have re...

Descripción completa

Detalles Bibliográficos
Autores principales: Nagao, Itsuma, Ambrosini, Yoko M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250713/
https://www.ncbi.nlm.nih.gov/pubmed/37303723
http://dx.doi.org/10.3389/fvets.2023.1179836
_version_ 1785055814057197568
author Nagao, Itsuma
Ambrosini, Yoko M.
author_facet Nagao, Itsuma
Ambrosini, Yoko M.
author_sort Nagao, Itsuma
collection PubMed
description The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have recently gained attention in the investigation of human diseases due to their remarkable similarities in physiology and pathophysiology with the human gallbladder. In this study, we have successfully established and characterized bovine gallbladder cholangiocyte organoids (GCOs) that retain key characteristics of the gallbladder in vivo, including stem cell properties and proliferative capacity. Notably, our findings demonstrate that these organoids exhibit specific and functional CFTR activity. We believe that these bovine GCOs represent a valuable tool for studying the physiology and pathophysiology of the gallbladder with human significance.
format Online
Article
Text
id pubmed-10250713
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102507132023-06-10 Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system Nagao, Itsuma Ambrosini, Yoko M. Front Vet Sci Veterinary Science The study of biliary physiology and pathophysiology has long been hindered by the lack of in vitro models that accurately reflect the complex functions of the biliary system. Recent advancements in 3D organoid technology may offer a promising solution to this issue. Bovine gallbladder models have recently gained attention in the investigation of human diseases due to their remarkable similarities in physiology and pathophysiology with the human gallbladder. In this study, we have successfully established and characterized bovine gallbladder cholangiocyte organoids (GCOs) that retain key characteristics of the gallbladder in vivo, including stem cell properties and proliferative capacity. Notably, our findings demonstrate that these organoids exhibit specific and functional CFTR activity. We believe that these bovine GCOs represent a valuable tool for studying the physiology and pathophysiology of the gallbladder with human significance. Frontiers Media S.A. 2023-05-26 /pmc/articles/PMC10250713/ /pubmed/37303723 http://dx.doi.org/10.3389/fvets.2023.1179836 Text en Copyright © 2023 Nagao and Ambrosini. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Nagao, Itsuma
Ambrosini, Yoko M.
Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
title Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
title_full Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
title_fullStr Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
title_full_unstemmed Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
title_short Ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
title_sort ion channel function in translational bovine gallbladder cholangiocyte organoids: establishment and characterization of a novel model system
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10250713/
https://www.ncbi.nlm.nih.gov/pubmed/37303723
http://dx.doi.org/10.3389/fvets.2023.1179836
work_keys_str_mv AT nagaoitsuma ionchannelfunctionintranslationalbovinegallbladdercholangiocyteorganoidsestablishmentandcharacterizationofanovelmodelsystem
AT ambrosiniyokom ionchannelfunctionintranslationalbovinegallbladdercholangiocyteorganoidsestablishmentandcharacterizationofanovelmodelsystem