Cargando…
Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
Acinetobacter baumannii is a critical pathogen with an efficient SOS (Save Our Ship) system that plays a significant role in antibiotic resistance. This prospective descriptive study aimed to investigate the association between expression levels of recA and umuDC genes, which are critical in SOS pat...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Carol Davila University Press
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251391/ https://www.ncbi.nlm.nih.gov/pubmed/37305826 http://dx.doi.org/10.25122/jml-2022-0358 |
_version_ | 1785055938376368128 |
---|---|
author | Ameer, Nada Ali Abdul Dhahi, Maysaa Abdul Razzaq |
author_facet | Ameer, Nada Ali Abdul Dhahi, Maysaa Abdul Razzaq |
author_sort | Ameer, Nada Ali Abdul |
collection | PubMed |
description | Acinetobacter baumannii is a critical pathogen with an efficient SOS (Save Our Ship) system that plays a significant role in antibiotic resistance. This prospective descriptive study aimed to investigate the association between expression levels of recA and umuDC genes, which are critical in SOS pathways, and antibiotic resistance in A. baumannii. We analyzed 78 clinical isolates and 31 ecological isolates using the Vitek-2 system for bacterial identification and antibiotic susceptibility testing and confirmed molecular identification of A. baumannii by conventional PCR of bla(OXA-51) and bla(OXA-23). Quantitative real-time polymerase chain reaction was used to determine gene expression levels of recA and umuDC. The results showed that in 25 clinical strains, 14/25 strains showed upregulation of recA, 7/25 strains exhibited upregulation of both umuDC and recA, and 1/25 strains showed upregulation of umuDC. Of these, 16/25 clinical strains were extensively resistant to antibiotics, except for colistin, and showed upregulation of recA and/or umuDC gene expression levels. In 6 ecological strains, recA showed upregulation in 3/6 strains, while both recA and umuDC were upregulated in 1/6 strain. In conclusion, high expression levels of recA and/or umuDC genes in A. baumannii complex and A. baumannii strains may contribute to increasing resistance to a wide range of antibiotics and may result in the initiation of an extensively drug-resistant (XDR) phenotype. |
format | Online Article Text |
id | pubmed-10251391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Carol Davila University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102513912023-06-10 Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii Ameer, Nada Ali Abdul Dhahi, Maysaa Abdul Razzaq J Med Life Original Article Acinetobacter baumannii is a critical pathogen with an efficient SOS (Save Our Ship) system that plays a significant role in antibiotic resistance. This prospective descriptive study aimed to investigate the association between expression levels of recA and umuDC genes, which are critical in SOS pathways, and antibiotic resistance in A. baumannii. We analyzed 78 clinical isolates and 31 ecological isolates using the Vitek-2 system for bacterial identification and antibiotic susceptibility testing and confirmed molecular identification of A. baumannii by conventional PCR of bla(OXA-51) and bla(OXA-23). Quantitative real-time polymerase chain reaction was used to determine gene expression levels of recA and umuDC. The results showed that in 25 clinical strains, 14/25 strains showed upregulation of recA, 7/25 strains exhibited upregulation of both umuDC and recA, and 1/25 strains showed upregulation of umuDC. Of these, 16/25 clinical strains were extensively resistant to antibiotics, except for colistin, and showed upregulation of recA and/or umuDC gene expression levels. In 6 ecological strains, recA showed upregulation in 3/6 strains, while both recA and umuDC were upregulated in 1/6 strain. In conclusion, high expression levels of recA and/or umuDC genes in A. baumannii complex and A. baumannii strains may contribute to increasing resistance to a wide range of antibiotics and may result in the initiation of an extensively drug-resistant (XDR) phenotype. Carol Davila University Press 2023-04 /pmc/articles/PMC10251391/ /pubmed/37305826 http://dx.doi.org/10.25122/jml-2022-0358 Text en ©2023 JOURNAL of MEDICINE and LIFE https://creativecommons.org/licenses/by/3.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Original Article Ameer, Nada Ali Abdul Dhahi, Maysaa Abdul Razzaq Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii |
title | Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii |
title_full | Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii |
title_fullStr | Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii |
title_full_unstemmed | Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii |
title_short | Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii |
title_sort | alterations in gene expression of reca and umudc in antibiotic-resistant acinetobacter baumannii |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251391/ https://www.ncbi.nlm.nih.gov/pubmed/37305826 http://dx.doi.org/10.25122/jml-2022-0358 |
work_keys_str_mv | AT ameernadaaliabdul alterationsingeneexpressionofrecaandumudcinantibioticresistantacinetobacterbaumannii AT dhahimaysaaabdulrazzaq alterationsingeneexpressionofrecaandumudcinantibioticresistantacinetobacterbaumannii |