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Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii

Acinetobacter baumannii is a critical pathogen with an efficient SOS (Save Our Ship) system that plays a significant role in antibiotic resistance. This prospective descriptive study aimed to investigate the association between expression levels of recA and umuDC genes, which are critical in SOS pat...

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Autores principales: Ameer, Nada Ali Abdul, Dhahi, Maysaa Abdul Razzaq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Carol Davila University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251391/
https://www.ncbi.nlm.nih.gov/pubmed/37305826
http://dx.doi.org/10.25122/jml-2022-0358
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author Ameer, Nada Ali Abdul
Dhahi, Maysaa Abdul Razzaq
author_facet Ameer, Nada Ali Abdul
Dhahi, Maysaa Abdul Razzaq
author_sort Ameer, Nada Ali Abdul
collection PubMed
description Acinetobacter baumannii is a critical pathogen with an efficient SOS (Save Our Ship) system that plays a significant role in antibiotic resistance. This prospective descriptive study aimed to investigate the association between expression levels of recA and umuDC genes, which are critical in SOS pathways, and antibiotic resistance in A. baumannii. We analyzed 78 clinical isolates and 31 ecological isolates using the Vitek-2 system for bacterial identification and antibiotic susceptibility testing and confirmed molecular identification of A. baumannii by conventional PCR of bla(OXA-51) and bla(OXA-23). Quantitative real-time polymerase chain reaction was used to determine gene expression levels of recA and umuDC. The results showed that in 25 clinical strains, 14/25 strains showed upregulation of recA, 7/25 strains exhibited upregulation of both umuDC and recA, and 1/25 strains showed upregulation of umuDC. Of these, 16/25 clinical strains were extensively resistant to antibiotics, except for colistin, and showed upregulation of recA and/or umuDC gene expression levels. In 6 ecological strains, recA showed upregulation in 3/6 strains, while both recA and umuDC were upregulated in 1/6 strain. In conclusion, high expression levels of recA and/or umuDC genes in A. baumannii complex and A. baumannii strains may contribute to increasing resistance to a wide range of antibiotics and may result in the initiation of an extensively drug-resistant (XDR) phenotype.
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spelling pubmed-102513912023-06-10 Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii Ameer, Nada Ali Abdul Dhahi, Maysaa Abdul Razzaq J Med Life Original Article Acinetobacter baumannii is a critical pathogen with an efficient SOS (Save Our Ship) system that plays a significant role in antibiotic resistance. This prospective descriptive study aimed to investigate the association between expression levels of recA and umuDC genes, which are critical in SOS pathways, and antibiotic resistance in A. baumannii. We analyzed 78 clinical isolates and 31 ecological isolates using the Vitek-2 system for bacterial identification and antibiotic susceptibility testing and confirmed molecular identification of A. baumannii by conventional PCR of bla(OXA-51) and bla(OXA-23). Quantitative real-time polymerase chain reaction was used to determine gene expression levels of recA and umuDC. The results showed that in 25 clinical strains, 14/25 strains showed upregulation of recA, 7/25 strains exhibited upregulation of both umuDC and recA, and 1/25 strains showed upregulation of umuDC. Of these, 16/25 clinical strains were extensively resistant to antibiotics, except for colistin, and showed upregulation of recA and/or umuDC gene expression levels. In 6 ecological strains, recA showed upregulation in 3/6 strains, while both recA and umuDC were upregulated in 1/6 strain. In conclusion, high expression levels of recA and/or umuDC genes in A. baumannii complex and A. baumannii strains may contribute to increasing resistance to a wide range of antibiotics and may result in the initiation of an extensively drug-resistant (XDR) phenotype. Carol Davila University Press 2023-04 /pmc/articles/PMC10251391/ /pubmed/37305826 http://dx.doi.org/10.25122/jml-2022-0358 Text en ©2023 JOURNAL of MEDICINE and LIFE https://creativecommons.org/licenses/by/3.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Original Article
Ameer, Nada Ali Abdul
Dhahi, Maysaa Abdul Razzaq
Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
title Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
title_full Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
title_fullStr Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
title_full_unstemmed Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
title_short Alterations in gene expression of recA and umuDC in antibiotic-resistant Acinetobacter baumannii
title_sort alterations in gene expression of reca and umudc in antibiotic-resistant acinetobacter baumannii
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251391/
https://www.ncbi.nlm.nih.gov/pubmed/37305826
http://dx.doi.org/10.25122/jml-2022-0358
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