Development of a Chemogenetic Approach to Manipulate Intracellular pH

[Image: see text] Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that...

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Autores principales: Ghaffari Zaki, Asal, Miri, Seyed Mohammad, Çimen, Şeyma, Akgül Çağlar, Tuba, Yiğit, Esra N., Aydın, Mehmet Ş., Öztürk, Gürkan, Eroglu, Emrah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251507/
https://www.ncbi.nlm.nih.gov/pubmed/37222194
http://dx.doi.org/10.1021/jacs.3c00703
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author Ghaffari Zaki, Asal
Miri, Seyed Mohammad
Çimen, Şeyma
Akgül Çağlar, Tuba
Yiğit, Esra N.
Aydın, Mehmet Ş.
Öztürk, Gürkan
Eroglu, Emrah
author_facet Ghaffari Zaki, Asal
Miri, Seyed Mohammad
Çimen, Şeyma
Akgül Çağlar, Tuba
Yiğit, Esra N.
Aydın, Mehmet Ş.
Öztürk, Gürkan
Eroglu, Emrah
author_sort Ghaffari Zaki, Asal
collection PubMed
description [Image: see text] Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively in the presence of β-chloro-d-alanine in living cells in a concentration-dependent manner. The pH-Control approach is promising for investigating the ultralocal pH imbalance associated with many diseases.
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spelling pubmed-102515072023-06-10 Development of a Chemogenetic Approach to Manipulate Intracellular pH Ghaffari Zaki, Asal Miri, Seyed Mohammad Çimen, Şeyma Akgül Çağlar, Tuba Yiğit, Esra N. Aydın, Mehmet Ş. Öztürk, Gürkan Eroglu, Emrah J Am Chem Soc [Image: see text] Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively in the presence of β-chloro-d-alanine in living cells in a concentration-dependent manner. The pH-Control approach is promising for investigating the ultralocal pH imbalance associated with many diseases. American Chemical Society 2023-05-24 /pmc/articles/PMC10251507/ /pubmed/37222194 http://dx.doi.org/10.1021/jacs.3c00703 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Ghaffari Zaki, Asal
Miri, Seyed Mohammad
Çimen, Şeyma
Akgül Çağlar, Tuba
Yiğit, Esra N.
Aydın, Mehmet Ş.
Öztürk, Gürkan
Eroglu, Emrah
Development of a Chemogenetic Approach to Manipulate Intracellular pH
title Development of a Chemogenetic Approach to Manipulate Intracellular pH
title_full Development of a Chemogenetic Approach to Manipulate Intracellular pH
title_fullStr Development of a Chemogenetic Approach to Manipulate Intracellular pH
title_full_unstemmed Development of a Chemogenetic Approach to Manipulate Intracellular pH
title_short Development of a Chemogenetic Approach to Manipulate Intracellular pH
title_sort development of a chemogenetic approach to manipulate intracellular ph
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251507/
https://www.ncbi.nlm.nih.gov/pubmed/37222194
http://dx.doi.org/10.1021/jacs.3c00703
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