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Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers

NEDD4 family represent an important group of E3 ligases, which regulate various cellular pathways of cell proliferation, cell junction and inflammation. Emerging evidence suggested that NEDD4 family members participate in the initiation and development of tumor. In this study, we systematically inve...

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Autores principales: Cao, Liangzi, Li, Hao, Liu, Xiaofang, Wang, Yubang, Zheng, Bowen, Xing, Chengzhong, Zhang, Naijin, Liu, Jingwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251597/
https://www.ncbi.nlm.nih.gov/pubmed/37291499
http://dx.doi.org/10.1186/s12885-023-11007-w
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author Cao, Liangzi
Li, Hao
Liu, Xiaofang
Wang, Yubang
Zheng, Bowen
Xing, Chengzhong
Zhang, Naijin
Liu, Jingwei
author_facet Cao, Liangzi
Li, Hao
Liu, Xiaofang
Wang, Yubang
Zheng, Bowen
Xing, Chengzhong
Zhang, Naijin
Liu, Jingwei
author_sort Cao, Liangzi
collection PubMed
description NEDD4 family represent an important group of E3 ligases, which regulate various cellular pathways of cell proliferation, cell junction and inflammation. Emerging evidence suggested that NEDD4 family members participate in the initiation and development of tumor. In this study, we systematically investigated the molecular alterations as well as the clinical relevance regarding NEDD4 family genes in 33 cancer types. Finally, we found that NEDD4 members showed increased expression in pancreas cancer and decreased expression in thyroid cancer. NEDD4 E3 ligase family genes had an average mutation frequency in the range of 0-32.1%, of which HECW1 and HECW2 demonstrated relatively high mutation rate. Breast cancer harbors large amount of NEDD4 copy number amplification. NEDD4 family members interacted proteins were enriched in various pathways including p53, Akt, apoptosis and autophagy, which were confirmed by further western blot and flow cytometric analysis in A549 and H1299 lung cancer cells. In addition, expression of NEDD4 family genes were associated with survival of cancer patients. Our findings provide novel insight into the effect of NEDD4 E3 ligase genes on cancer progression and treatment in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-023-11007-w.
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spelling pubmed-102515972023-06-10 Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers Cao, Liangzi Li, Hao Liu, Xiaofang Wang, Yubang Zheng, Bowen Xing, Chengzhong Zhang, Naijin Liu, Jingwei BMC Cancer Research NEDD4 family represent an important group of E3 ligases, which regulate various cellular pathways of cell proliferation, cell junction and inflammation. Emerging evidence suggested that NEDD4 family members participate in the initiation and development of tumor. In this study, we systematically investigated the molecular alterations as well as the clinical relevance regarding NEDD4 family genes in 33 cancer types. Finally, we found that NEDD4 members showed increased expression in pancreas cancer and decreased expression in thyroid cancer. NEDD4 E3 ligase family genes had an average mutation frequency in the range of 0-32.1%, of which HECW1 and HECW2 demonstrated relatively high mutation rate. Breast cancer harbors large amount of NEDD4 copy number amplification. NEDD4 family members interacted proteins were enriched in various pathways including p53, Akt, apoptosis and autophagy, which were confirmed by further western blot and flow cytometric analysis in A549 and H1299 lung cancer cells. In addition, expression of NEDD4 family genes were associated with survival of cancer patients. Our findings provide novel insight into the effect of NEDD4 E3 ligase genes on cancer progression and treatment in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-023-11007-w. BioMed Central 2023-06-08 /pmc/articles/PMC10251597/ /pubmed/37291499 http://dx.doi.org/10.1186/s12885-023-11007-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Cao, Liangzi
Li, Hao
Liu, Xiaofang
Wang, Yubang
Zheng, Bowen
Xing, Chengzhong
Zhang, Naijin
Liu, Jingwei
Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers
title Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers
title_full Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers
title_fullStr Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers
title_full_unstemmed Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers
title_short Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers
title_sort expression and regulatory network of e3 ubiquitin ligase nedd4 family in cancers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10251597/
https://www.ncbi.nlm.nih.gov/pubmed/37291499
http://dx.doi.org/10.1186/s12885-023-11007-w
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