Cargando…

Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study

SIMPLE SUMMARY: Breast cancer is the second most commonly diagnosed cancer among women. Novel breast cancer screening techniques are necessary to improve early detection and increase the chances of successful treatment. This study investigates the presence of specific volatile organic compounds in t...

Descripción completa

Detalles Bibliográficos
Autores principales: Leemans, Michelle, Cuzuel, Vincent, Bauër, Pierre, Baba Aissa, Hind, Cournelle, Gabriel, Baelde, Aurélien, Thuleau, Aurélie, Cognon, Guillaume, Pouget, Nicolas, Guillot, Eugénie, Fromantin, Isabelle, Audureau, Etienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252040/
https://www.ncbi.nlm.nih.gov/pubmed/37296901
http://dx.doi.org/10.3390/cancers15112939
_version_ 1785056075938004992
author Leemans, Michelle
Cuzuel, Vincent
Bauër, Pierre
Baba Aissa, Hind
Cournelle, Gabriel
Baelde, Aurélien
Thuleau, Aurélie
Cognon, Guillaume
Pouget, Nicolas
Guillot, Eugénie
Fromantin, Isabelle
Audureau, Etienne
author_facet Leemans, Michelle
Cuzuel, Vincent
Bauër, Pierre
Baba Aissa, Hind
Cournelle, Gabriel
Baelde, Aurélien
Thuleau, Aurélie
Cognon, Guillaume
Pouget, Nicolas
Guillot, Eugénie
Fromantin, Isabelle
Audureau, Etienne
author_sort Leemans, Michelle
collection PubMed
description SIMPLE SUMMARY: Breast cancer is the second most commonly diagnosed cancer among women. Novel breast cancer screening techniques are necessary to improve early detection and increase the chances of successful treatment. This study investigates the presence of specific volatile organic compounds in the sweat of breast cancer patients, which could serve as biomarkers for non-invasive screening. Sweat samples were collected from 21 breast cancer patients before and after tumor removal surgery and analyzed using thermal desorption coupled with gas chromatography and mass spectrometry. This work demonstrates that volatile organic compounds in sweat differ between pre-and post-surgery status in breast cancer patients, suggesting the possibility to use these compounds as novel breast cancer biomarkers and opening the way to new screening tools. ABSTRACT: Breast cancer (BC) remains one of the most commonly diagnosed malignancies in women. There is increasing interest in the development of non-invasive screening methods. Volatile organic compounds (VOCs) emitted through the metabolism of cancer cells are possible novel cancer biomarkers. This study aims to identify the existence of BC-specific VOCs in the sweat of BC patients. Sweat samples from the breast and hand area were collected from 21 BC participants before and after breast tumor ablation. Thermal desorption coupled with two-dimensional gas chromatography and mass spectrometry was used to analyze VOCs. A total of 761 volatiles from a homemade human odor library were screened on each chromatogram. From those 761 VOCs, a minimum of 77 VOCs were detected within the BC samples. Principal component analysis showed that VOCs differ between the pre- and post-surgery status of the BC patients. The Tree-based Pipeline Optimization Tool identified logistic regression as the best-performing machine learning model. Logistic regression modeling identified VOCs that distinguish the pre-and post-surgery state in BC patients on both the breast and hand area with sensitivities close to 1. Further, Shapley additive explanations and the probe variable method identified the most important and pertinent VOCs distinguishing pre- and post-operative status which are mostly of distinct origin for the hand and breast region. Results suggest the possibility to identify endogenous metabolites linked to BC, hence proposing this innovative pipeline as a stepstone to discovering potential BC biomarkers. Large-scale studies in a multi-centered VOC analysis setting must be carried out to validate obtained findings.
format Online
Article
Text
id pubmed-10252040
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102520402023-06-10 Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study Leemans, Michelle Cuzuel, Vincent Bauër, Pierre Baba Aissa, Hind Cournelle, Gabriel Baelde, Aurélien Thuleau, Aurélie Cognon, Guillaume Pouget, Nicolas Guillot, Eugénie Fromantin, Isabelle Audureau, Etienne Cancers (Basel) Article SIMPLE SUMMARY: Breast cancer is the second most commonly diagnosed cancer among women. Novel breast cancer screening techniques are necessary to improve early detection and increase the chances of successful treatment. This study investigates the presence of specific volatile organic compounds in the sweat of breast cancer patients, which could serve as biomarkers for non-invasive screening. Sweat samples were collected from 21 breast cancer patients before and after tumor removal surgery and analyzed using thermal desorption coupled with gas chromatography and mass spectrometry. This work demonstrates that volatile organic compounds in sweat differ between pre-and post-surgery status in breast cancer patients, suggesting the possibility to use these compounds as novel breast cancer biomarkers and opening the way to new screening tools. ABSTRACT: Breast cancer (BC) remains one of the most commonly diagnosed malignancies in women. There is increasing interest in the development of non-invasive screening methods. Volatile organic compounds (VOCs) emitted through the metabolism of cancer cells are possible novel cancer biomarkers. This study aims to identify the existence of BC-specific VOCs in the sweat of BC patients. Sweat samples from the breast and hand area were collected from 21 BC participants before and after breast tumor ablation. Thermal desorption coupled with two-dimensional gas chromatography and mass spectrometry was used to analyze VOCs. A total of 761 volatiles from a homemade human odor library were screened on each chromatogram. From those 761 VOCs, a minimum of 77 VOCs were detected within the BC samples. Principal component analysis showed that VOCs differ between the pre- and post-surgery status of the BC patients. The Tree-based Pipeline Optimization Tool identified logistic regression as the best-performing machine learning model. Logistic regression modeling identified VOCs that distinguish the pre-and post-surgery state in BC patients on both the breast and hand area with sensitivities close to 1. Further, Shapley additive explanations and the probe variable method identified the most important and pertinent VOCs distinguishing pre- and post-operative status which are mostly of distinct origin for the hand and breast region. Results suggest the possibility to identify endogenous metabolites linked to BC, hence proposing this innovative pipeline as a stepstone to discovering potential BC biomarkers. Large-scale studies in a multi-centered VOC analysis setting must be carried out to validate obtained findings. MDPI 2023-05-26 /pmc/articles/PMC10252040/ /pubmed/37296901 http://dx.doi.org/10.3390/cancers15112939 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leemans, Michelle
Cuzuel, Vincent
Bauër, Pierre
Baba Aissa, Hind
Cournelle, Gabriel
Baelde, Aurélien
Thuleau, Aurélie
Cognon, Guillaume
Pouget, Nicolas
Guillot, Eugénie
Fromantin, Isabelle
Audureau, Etienne
Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study
title Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study
title_full Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study
title_fullStr Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study
title_full_unstemmed Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study
title_short Screening of Breast Cancer from Sweat Samples Analyzed by 2-Dimensional Gas Chromatography-Mass Spectrometry: A Preliminary Study
title_sort screening of breast cancer from sweat samples analyzed by 2-dimensional gas chromatography-mass spectrometry: a preliminary study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252040/
https://www.ncbi.nlm.nih.gov/pubmed/37296901
http://dx.doi.org/10.3390/cancers15112939
work_keys_str_mv AT leemansmichelle screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT cuzuelvincent screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT bauerpierre screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT babaaissahind screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT cournellegabriel screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT baeldeaurelien screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT thuleauaurelie screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT cognonguillaume screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT pougetnicolas screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT guilloteugenie screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT fromantinisabelle screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy
AT audureauetienne screeningofbreastcancerfromsweatsamplesanalyzedby2dimensionalgaschromatographymassspectrometryapreliminarystudy