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Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow
Blood flow in the microcirculatory system is crucially affected by intrinsic red blood cell (RBC) properties, such as their deformability. In the smallest vessels of this network, RBCs adapt their shapes to the flow conditions. Although it is known that the age of RBCs modifies their physical proper...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252257/ https://www.ncbi.nlm.nih.gov/pubmed/37296651 http://dx.doi.org/10.3390/cells12111529 |
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author | Nouaman, Mohammed Darras, Alexis John, Thomas Simionato, Greta Rab, Minke A. E. van Wijk, Richard Laschke, Matthias W. Kaestner, Lars Wagner, Christian Recktenwald, Steffen M. |
author_facet | Nouaman, Mohammed Darras, Alexis John, Thomas Simionato, Greta Rab, Minke A. E. van Wijk, Richard Laschke, Matthias W. Kaestner, Lars Wagner, Christian Recktenwald, Steffen M. |
author_sort | Nouaman, Mohammed |
collection | PubMed |
description | Blood flow in the microcirculatory system is crucially affected by intrinsic red blood cell (RBC) properties, such as their deformability. In the smallest vessels of this network, RBCs adapt their shapes to the flow conditions. Although it is known that the age of RBCs modifies their physical properties, such as increased cytosol viscosity and altered viscoelastic membrane properties, the evolution of their shape-adapting abilities during senescence remains unclear. In this study, we investigated the effect of RBC properties on the microcapillary in vitro flow behavior and their characteristic shapes in microfluidic channels. For this, we fractioned RBCs from healthy donors according to their age. Moreover, the membranes of fresh RBCs were chemically rigidified using diamide to study the effect of isolated graded-membrane rigidity. Our results show that a fraction of stable, asymmetric, off-centered slipper-like cells at high velocities decreases with increasing age or diamide concentration. However, while old cells form an enhanced number of stable symmetric croissants at the channel centerline, this shape class is suppressed for purely rigidified cells with diamide. Our study provides further knowledge about the distinct effects of age-related changes of intrinsic cell properties on the single-cell flow behavior of RBCs in confined flows due to inter-cellular age-related cell heterogeneity. |
format | Online Article Text |
id | pubmed-10252257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102522572023-06-10 Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow Nouaman, Mohammed Darras, Alexis John, Thomas Simionato, Greta Rab, Minke A. E. van Wijk, Richard Laschke, Matthias W. Kaestner, Lars Wagner, Christian Recktenwald, Steffen M. Cells Article Blood flow in the microcirculatory system is crucially affected by intrinsic red blood cell (RBC) properties, such as their deformability. In the smallest vessels of this network, RBCs adapt their shapes to the flow conditions. Although it is known that the age of RBCs modifies their physical properties, such as increased cytosol viscosity and altered viscoelastic membrane properties, the evolution of their shape-adapting abilities during senescence remains unclear. In this study, we investigated the effect of RBC properties on the microcapillary in vitro flow behavior and their characteristic shapes in microfluidic channels. For this, we fractioned RBCs from healthy donors according to their age. Moreover, the membranes of fresh RBCs were chemically rigidified using diamide to study the effect of isolated graded-membrane rigidity. Our results show that a fraction of stable, asymmetric, off-centered slipper-like cells at high velocities decreases with increasing age or diamide concentration. However, while old cells form an enhanced number of stable symmetric croissants at the channel centerline, this shape class is suppressed for purely rigidified cells with diamide. Our study provides further knowledge about the distinct effects of age-related changes of intrinsic cell properties on the single-cell flow behavior of RBCs in confined flows due to inter-cellular age-related cell heterogeneity. MDPI 2023-06-01 /pmc/articles/PMC10252257/ /pubmed/37296651 http://dx.doi.org/10.3390/cells12111529 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nouaman, Mohammed Darras, Alexis John, Thomas Simionato, Greta Rab, Minke A. E. van Wijk, Richard Laschke, Matthias W. Kaestner, Lars Wagner, Christian Recktenwald, Steffen M. Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow |
title | Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow |
title_full | Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow |
title_fullStr | Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow |
title_full_unstemmed | Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow |
title_short | Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow |
title_sort | effect of cell age and membrane rigidity on red blood cell shape in capillary flow |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252257/ https://www.ncbi.nlm.nih.gov/pubmed/37296651 http://dx.doi.org/10.3390/cells12111529 |
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