Cargando…
Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations....
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252311/ https://www.ncbi.nlm.nih.gov/pubmed/37296657 http://dx.doi.org/10.3390/cells12111537 |
_version_ | 1785056140143362048 |
---|---|
author | Roy, Neena Lazzaretti, Clara Paradiso, Elia Capponi, Chiara Ferrari, Tommaso Reggianini, Francesca Sperduti, Samantha Baschieri, Lara Mascolo, Elisa Perri, Carmela Varani, Manuela Canu, Giulia Trenti, Tommaso Nicoli, Alessia Morini, Daria Iannotti, Francesca Villani, Maria Teresa Vicini, Elena Simoni, Manuela Casarini, Livio |
author_facet | Roy, Neena Lazzaretti, Clara Paradiso, Elia Capponi, Chiara Ferrari, Tommaso Reggianini, Francesca Sperduti, Samantha Baschieri, Lara Mascolo, Elisa Perri, Carmela Varani, Manuela Canu, Giulia Trenti, Tommaso Nicoli, Alessia Morini, Daria Iannotti, Francesca Villani, Maria Teresa Vicini, Elena Simoni, Manuela Casarini, Livio |
author_sort | Roy, Neena |
collection | PubMed |
description | Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations. However, the impact of short-term exposure to BPA on reproduction is poorly studied. We evaluated if 8 and 24 h exposure to 1 nM and 1 µM BPA perturbs luteinizing hormone/choriogonadotropin (LH/hCG)-mediated signalling in two steroidogenic cell models, i.e., the mouse tumour Leydig cell line mLTC1, and human primary granulosa lutein cells (hGLC). Cell signalling studies were performed using a homogeneous time-resolved fluorescence (HTRF) assay and Western blotting, while gene expression analysis was carried out using real-time PCR. Immunostainings and an immunoassay were used for intracellular protein expression and steroidogenesis analyses, respectively. The presence of BPA leads to no significant changes in gonadotropin-induced cAMP accumulation, alongside phosphorylation of downstream molecules, such as ERK1/2, CREB and p38 MAPK, in both the cell models. BPA did not impact STARD1, CYP11A1 and CYP19A1 gene expression in hGLC, nor Stard1 and Cyp17a1 expression in mLTC1 treated with LH/hCG. Additionally, the StAR protein expression was unchanged upon exposure to BPA. Progesterone and oestradiol levels in the culture medium, measured by hGLC, as well as the testosterone and progesterone levels in the culture medium, measured by mLTC1, did not change in the presence of BPA combined with LH/hCG. These data suggest that short-term exposure to environmental concentrations of BPA does not compromise the LH/hCG-induced steroidogenic potential of either human granulosa or mouse Leydig cells. |
format | Online Article Text |
id | pubmed-10252311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102523112023-06-10 Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro Roy, Neena Lazzaretti, Clara Paradiso, Elia Capponi, Chiara Ferrari, Tommaso Reggianini, Francesca Sperduti, Samantha Baschieri, Lara Mascolo, Elisa Perri, Carmela Varani, Manuela Canu, Giulia Trenti, Tommaso Nicoli, Alessia Morini, Daria Iannotti, Francesca Villani, Maria Teresa Vicini, Elena Simoni, Manuela Casarini, Livio Cells Article Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations. However, the impact of short-term exposure to BPA on reproduction is poorly studied. We evaluated if 8 and 24 h exposure to 1 nM and 1 µM BPA perturbs luteinizing hormone/choriogonadotropin (LH/hCG)-mediated signalling in two steroidogenic cell models, i.e., the mouse tumour Leydig cell line mLTC1, and human primary granulosa lutein cells (hGLC). Cell signalling studies were performed using a homogeneous time-resolved fluorescence (HTRF) assay and Western blotting, while gene expression analysis was carried out using real-time PCR. Immunostainings and an immunoassay were used for intracellular protein expression and steroidogenesis analyses, respectively. The presence of BPA leads to no significant changes in gonadotropin-induced cAMP accumulation, alongside phosphorylation of downstream molecules, such as ERK1/2, CREB and p38 MAPK, in both the cell models. BPA did not impact STARD1, CYP11A1 and CYP19A1 gene expression in hGLC, nor Stard1 and Cyp17a1 expression in mLTC1 treated with LH/hCG. Additionally, the StAR protein expression was unchanged upon exposure to BPA. Progesterone and oestradiol levels in the culture medium, measured by hGLC, as well as the testosterone and progesterone levels in the culture medium, measured by mLTC1, did not change in the presence of BPA combined with LH/hCG. These data suggest that short-term exposure to environmental concentrations of BPA does not compromise the LH/hCG-induced steroidogenic potential of either human granulosa or mouse Leydig cells. MDPI 2023-06-02 /pmc/articles/PMC10252311/ /pubmed/37296657 http://dx.doi.org/10.3390/cells12111537 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Roy, Neena Lazzaretti, Clara Paradiso, Elia Capponi, Chiara Ferrari, Tommaso Reggianini, Francesca Sperduti, Samantha Baschieri, Lara Mascolo, Elisa Perri, Carmela Varani, Manuela Canu, Giulia Trenti, Tommaso Nicoli, Alessia Morini, Daria Iannotti, Francesca Villani, Maria Teresa Vicini, Elena Simoni, Manuela Casarini, Livio Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro |
title | Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro |
title_full | Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro |
title_fullStr | Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro |
title_full_unstemmed | Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro |
title_short | Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro |
title_sort | short-term exposure to bisphenol a does not impact gonadal cell steroidogenesis in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252311/ https://www.ncbi.nlm.nih.gov/pubmed/37296657 http://dx.doi.org/10.3390/cells12111537 |
work_keys_str_mv | AT royneena shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT lazzaretticlara shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT paradisoelia shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT capponichiara shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT ferraritommaso shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT reggianinifrancesca shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT sperdutisamantha shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT baschierilara shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT mascoloelisa shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT perricarmela shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT varanimanuela shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT canugiulia shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT trentitommaso shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT nicolialessia shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT morinidaria shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT iannottifrancesca shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT villanimariateresa shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT vicinielena shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT simonimanuela shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro AT casarinilivio shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro |