Cargando…

Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro

Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations....

Descripción completa

Detalles Bibliográficos
Autores principales: Roy, Neena, Lazzaretti, Clara, Paradiso, Elia, Capponi, Chiara, Ferrari, Tommaso, Reggianini, Francesca, Sperduti, Samantha, Baschieri, Lara, Mascolo, Elisa, Perri, Carmela, Varani, Manuela, Canu, Giulia, Trenti, Tommaso, Nicoli, Alessia, Morini, Daria, Iannotti, Francesca, Villani, Maria Teresa, Vicini, Elena, Simoni, Manuela, Casarini, Livio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252311/
https://www.ncbi.nlm.nih.gov/pubmed/37296657
http://dx.doi.org/10.3390/cells12111537
_version_ 1785056140143362048
author Roy, Neena
Lazzaretti, Clara
Paradiso, Elia
Capponi, Chiara
Ferrari, Tommaso
Reggianini, Francesca
Sperduti, Samantha
Baschieri, Lara
Mascolo, Elisa
Perri, Carmela
Varani, Manuela
Canu, Giulia
Trenti, Tommaso
Nicoli, Alessia
Morini, Daria
Iannotti, Francesca
Villani, Maria Teresa
Vicini, Elena
Simoni, Manuela
Casarini, Livio
author_facet Roy, Neena
Lazzaretti, Clara
Paradiso, Elia
Capponi, Chiara
Ferrari, Tommaso
Reggianini, Francesca
Sperduti, Samantha
Baschieri, Lara
Mascolo, Elisa
Perri, Carmela
Varani, Manuela
Canu, Giulia
Trenti, Tommaso
Nicoli, Alessia
Morini, Daria
Iannotti, Francesca
Villani, Maria Teresa
Vicini, Elena
Simoni, Manuela
Casarini, Livio
author_sort Roy, Neena
collection PubMed
description Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations. However, the impact of short-term exposure to BPA on reproduction is poorly studied. We evaluated if 8 and 24 h exposure to 1 nM and 1 µM BPA perturbs luteinizing hormone/choriogonadotropin (LH/hCG)-mediated signalling in two steroidogenic cell models, i.e., the mouse tumour Leydig cell line mLTC1, and human primary granulosa lutein cells (hGLC). Cell signalling studies were performed using a homogeneous time-resolved fluorescence (HTRF) assay and Western blotting, while gene expression analysis was carried out using real-time PCR. Immunostainings and an immunoassay were used for intracellular protein expression and steroidogenesis analyses, respectively. The presence of BPA leads to no significant changes in gonadotropin-induced cAMP accumulation, alongside phosphorylation of downstream molecules, such as ERK1/2, CREB and p38 MAPK, in both the cell models. BPA did not impact STARD1, CYP11A1 and CYP19A1 gene expression in hGLC, nor Stard1 and Cyp17a1 expression in mLTC1 treated with LH/hCG. Additionally, the StAR protein expression was unchanged upon exposure to BPA. Progesterone and oestradiol levels in the culture medium, measured by hGLC, as well as the testosterone and progesterone levels in the culture medium, measured by mLTC1, did not change in the presence of BPA combined with LH/hCG. These data suggest that short-term exposure to environmental concentrations of BPA does not compromise the LH/hCG-induced steroidogenic potential of either human granulosa or mouse Leydig cells.
format Online
Article
Text
id pubmed-10252311
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102523112023-06-10 Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro Roy, Neena Lazzaretti, Clara Paradiso, Elia Capponi, Chiara Ferrari, Tommaso Reggianini, Francesca Sperduti, Samantha Baschieri, Lara Mascolo, Elisa Perri, Carmela Varani, Manuela Canu, Giulia Trenti, Tommaso Nicoli, Alessia Morini, Daria Iannotti, Francesca Villani, Maria Teresa Vicini, Elena Simoni, Manuela Casarini, Livio Cells Article Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations. However, the impact of short-term exposure to BPA on reproduction is poorly studied. We evaluated if 8 and 24 h exposure to 1 nM and 1 µM BPA perturbs luteinizing hormone/choriogonadotropin (LH/hCG)-mediated signalling in two steroidogenic cell models, i.e., the mouse tumour Leydig cell line mLTC1, and human primary granulosa lutein cells (hGLC). Cell signalling studies were performed using a homogeneous time-resolved fluorescence (HTRF) assay and Western blotting, while gene expression analysis was carried out using real-time PCR. Immunostainings and an immunoassay were used for intracellular protein expression and steroidogenesis analyses, respectively. The presence of BPA leads to no significant changes in gonadotropin-induced cAMP accumulation, alongside phosphorylation of downstream molecules, such as ERK1/2, CREB and p38 MAPK, in both the cell models. BPA did not impact STARD1, CYP11A1 and CYP19A1 gene expression in hGLC, nor Stard1 and Cyp17a1 expression in mLTC1 treated with LH/hCG. Additionally, the StAR protein expression was unchanged upon exposure to BPA. Progesterone and oestradiol levels in the culture medium, measured by hGLC, as well as the testosterone and progesterone levels in the culture medium, measured by mLTC1, did not change in the presence of BPA combined with LH/hCG. These data suggest that short-term exposure to environmental concentrations of BPA does not compromise the LH/hCG-induced steroidogenic potential of either human granulosa or mouse Leydig cells. MDPI 2023-06-02 /pmc/articles/PMC10252311/ /pubmed/37296657 http://dx.doi.org/10.3390/cells12111537 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roy, Neena
Lazzaretti, Clara
Paradiso, Elia
Capponi, Chiara
Ferrari, Tommaso
Reggianini, Francesca
Sperduti, Samantha
Baschieri, Lara
Mascolo, Elisa
Perri, Carmela
Varani, Manuela
Canu, Giulia
Trenti, Tommaso
Nicoli, Alessia
Morini, Daria
Iannotti, Francesca
Villani, Maria Teresa
Vicini, Elena
Simoni, Manuela
Casarini, Livio
Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
title Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
title_full Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
title_fullStr Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
title_full_unstemmed Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
title_short Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro
title_sort short-term exposure to bisphenol a does not impact gonadal cell steroidogenesis in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252311/
https://www.ncbi.nlm.nih.gov/pubmed/37296657
http://dx.doi.org/10.3390/cells12111537
work_keys_str_mv AT royneena shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT lazzaretticlara shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT paradisoelia shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT capponichiara shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT ferraritommaso shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT reggianinifrancesca shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT sperdutisamantha shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT baschierilara shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT mascoloelisa shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT perricarmela shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT varanimanuela shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT canugiulia shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT trentitommaso shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT nicolialessia shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT morinidaria shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT iannottifrancesca shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT villanimariateresa shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT vicinielena shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT simonimanuela shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro
AT casarinilivio shorttermexposuretobisphenoladoesnotimpactgonadalcellsteroidogenesisinvitro