Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica

Nitrate is one of the main sources of nitrogen for plant growth. Nitrate transporters (NRTs) participate in nitrate uptake and transport, and they are involved in abiotic stress tolerance. Previous studies have shown that NRT1.1 has a dual role in nitrate uptake and utilization; however, little is k...

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Detalles Bibliográficos
Autores principales: Liu, Guodong, Rui, Lin, Yang, Yuying, Liu, Ranxin, Li, Hongliang, Ye, Fan, You, Chunxiang, Zhang, Shuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252592/
https://www.ncbi.nlm.nih.gov/pubmed/37298242
http://dx.doi.org/10.3390/ijms24119291
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author Liu, Guodong
Rui, Lin
Yang, Yuying
Liu, Ranxin
Li, Hongliang
Ye, Fan
You, Chunxiang
Zhang, Shuai
author_facet Liu, Guodong
Rui, Lin
Yang, Yuying
Liu, Ranxin
Li, Hongliang
Ye, Fan
You, Chunxiang
Zhang, Shuai
author_sort Liu, Guodong
collection PubMed
description Nitrate is one of the main sources of nitrogen for plant growth. Nitrate transporters (NRTs) participate in nitrate uptake and transport, and they are involved in abiotic stress tolerance. Previous studies have shown that NRT1.1 has a dual role in nitrate uptake and utilization; however, little is known about the function of MdNRT1.1 in regulating apple growth and nitrate uptake. In this study, apple MdNRT1.1, a homolog of Arabidopsis NRT1.1, was cloned and functionally identified. Nitrate treatment induced an increased transcript level of MdNRT1.1, and overexpression of MdNRT1.1 promoted root development and nitrogen utilization. Ectopic expression of MdNRT1.1 in Arabidopsis repressed tolerance to drought, salt, and ABA stresses. Overall, this study identified a nitrate transporter, MdNRT1.1, in apples and revealed how MdNRT1.1 regulates nitrate utilization and abiotic stress tolerance.
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spelling pubmed-102525922023-06-10 Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica Liu, Guodong Rui, Lin Yang, Yuying Liu, Ranxin Li, Hongliang Ye, Fan You, Chunxiang Zhang, Shuai Int J Mol Sci Article Nitrate is one of the main sources of nitrogen for plant growth. Nitrate transporters (NRTs) participate in nitrate uptake and transport, and they are involved in abiotic stress tolerance. Previous studies have shown that NRT1.1 has a dual role in nitrate uptake and utilization; however, little is known about the function of MdNRT1.1 in regulating apple growth and nitrate uptake. In this study, apple MdNRT1.1, a homolog of Arabidopsis NRT1.1, was cloned and functionally identified. Nitrate treatment induced an increased transcript level of MdNRT1.1, and overexpression of MdNRT1.1 promoted root development and nitrogen utilization. Ectopic expression of MdNRT1.1 in Arabidopsis repressed tolerance to drought, salt, and ABA stresses. Overall, this study identified a nitrate transporter, MdNRT1.1, in apples and revealed how MdNRT1.1 regulates nitrate utilization and abiotic stress tolerance. MDPI 2023-05-26 /pmc/articles/PMC10252592/ /pubmed/37298242 http://dx.doi.org/10.3390/ijms24119291 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Guodong
Rui, Lin
Yang, Yuying
Liu, Ranxin
Li, Hongliang
Ye, Fan
You, Chunxiang
Zhang, Shuai
Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
title Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
title_full Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
title_fullStr Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
title_full_unstemmed Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
title_short Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica
title_sort identification and functional characterization of mdnrt1.1 in nitrogen utilization and abiotic stress tolerance in malus domestica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252592/
https://www.ncbi.nlm.nih.gov/pubmed/37298242
http://dx.doi.org/10.3390/ijms24119291
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