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Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling

Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have...

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Autores principales: D’Onofrio, Federica, Schirone, Maria, Paparella, Antonello, Krasteva, Ivanka, Tittarelli, Manuela, Pomilio, Francesco, Iannetti, Luigi, D’Alterio, Nicola, Luciani, Mirella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252599/
https://www.ncbi.nlm.nih.gov/pubmed/37297410
http://dx.doi.org/10.3390/foods12112166
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author D’Onofrio, Federica
Schirone, Maria
Paparella, Antonello
Krasteva, Ivanka
Tittarelli, Manuela
Pomilio, Francesco
Iannetti, Luigi
D’Alterio, Nicola
Luciani, Mirella
author_facet D’Onofrio, Federica
Schirone, Maria
Paparella, Antonello
Krasteva, Ivanka
Tittarelli, Manuela
Pomilio, Francesco
Iannetti, Luigi
D’Alterio, Nicola
Luciani, Mirella
author_sort D’Onofrio, Federica
collection PubMed
description Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have been published about proteome adaptation when L. monocytogenes is cultivated in stress conditions. In this study, we applied one-dimensional electrophoresis and 2D-PAGE combined with tandem mass spectrometry to evaluate proteome profiling in the following conditions: mild acid, low temperature, and high NaCl concentration. The total proteome was analyzed, also considering the case of normal growth-supporting conditions. A total of 1,160 proteins were identified and those related to pathogenesis and stress response pathways were analyzed. The proteins involved in the expression of virulent pathways when L. monocytogenes ST7 strain was grown under different stress conditions were described. Certain proteins, particularly those involved in the pathogenesis pathway, such as Listeriolysin regulatory protein and Internalin A, were only found when the strain was grown under specific stress conditions. Studying how L. monocytogenes adapts to stress can help to control its growth in food, reducing the risk for consumers.
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spelling pubmed-102525992023-06-10 Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling D’Onofrio, Federica Schirone, Maria Paparella, Antonello Krasteva, Ivanka Tittarelli, Manuela Pomilio, Francesco Iannetti, Luigi D’Alterio, Nicola Luciani, Mirella Foods Article Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have been published about proteome adaptation when L. monocytogenes is cultivated in stress conditions. In this study, we applied one-dimensional electrophoresis and 2D-PAGE combined with tandem mass spectrometry to evaluate proteome profiling in the following conditions: mild acid, low temperature, and high NaCl concentration. The total proteome was analyzed, also considering the case of normal growth-supporting conditions. A total of 1,160 proteins were identified and those related to pathogenesis and stress response pathways were analyzed. The proteins involved in the expression of virulent pathways when L. monocytogenes ST7 strain was grown under different stress conditions were described. Certain proteins, particularly those involved in the pathogenesis pathway, such as Listeriolysin regulatory protein and Internalin A, were only found when the strain was grown under specific stress conditions. Studying how L. monocytogenes adapts to stress can help to control its growth in food, reducing the risk for consumers. MDPI 2023-05-27 /pmc/articles/PMC10252599/ /pubmed/37297410 http://dx.doi.org/10.3390/foods12112166 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Onofrio, Federica
Schirone, Maria
Paparella, Antonello
Krasteva, Ivanka
Tittarelli, Manuela
Pomilio, Francesco
Iannetti, Luigi
D’Alterio, Nicola
Luciani, Mirella
Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
title Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
title_full Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
title_fullStr Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
title_full_unstemmed Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
title_short Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
title_sort stress adaptation responses of a listeria monocytogenes 1/2a strain via proteome profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252599/
https://www.ncbi.nlm.nih.gov/pubmed/37297410
http://dx.doi.org/10.3390/foods12112166
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