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Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252599/ https://www.ncbi.nlm.nih.gov/pubmed/37297410 http://dx.doi.org/10.3390/foods12112166 |
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author | D’Onofrio, Federica Schirone, Maria Paparella, Antonello Krasteva, Ivanka Tittarelli, Manuela Pomilio, Francesco Iannetti, Luigi D’Alterio, Nicola Luciani, Mirella |
author_facet | D’Onofrio, Federica Schirone, Maria Paparella, Antonello Krasteva, Ivanka Tittarelli, Manuela Pomilio, Francesco Iannetti, Luigi D’Alterio, Nicola Luciani, Mirella |
author_sort | D’Onofrio, Federica |
collection | PubMed |
description | Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have been published about proteome adaptation when L. monocytogenes is cultivated in stress conditions. In this study, we applied one-dimensional electrophoresis and 2D-PAGE combined with tandem mass spectrometry to evaluate proteome profiling in the following conditions: mild acid, low temperature, and high NaCl concentration. The total proteome was analyzed, also considering the case of normal growth-supporting conditions. A total of 1,160 proteins were identified and those related to pathogenesis and stress response pathways were analyzed. The proteins involved in the expression of virulent pathways when L. monocytogenes ST7 strain was grown under different stress conditions were described. Certain proteins, particularly those involved in the pathogenesis pathway, such as Listeriolysin regulatory protein and Internalin A, were only found when the strain was grown under specific stress conditions. Studying how L. monocytogenes adapts to stress can help to control its growth in food, reducing the risk for consumers. |
format | Online Article Text |
id | pubmed-10252599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102525992023-06-10 Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling D’Onofrio, Federica Schirone, Maria Paparella, Antonello Krasteva, Ivanka Tittarelli, Manuela Pomilio, Francesco Iannetti, Luigi D’Alterio, Nicola Luciani, Mirella Foods Article Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have been published about proteome adaptation when L. monocytogenes is cultivated in stress conditions. In this study, we applied one-dimensional electrophoresis and 2D-PAGE combined with tandem mass spectrometry to evaluate proteome profiling in the following conditions: mild acid, low temperature, and high NaCl concentration. The total proteome was analyzed, also considering the case of normal growth-supporting conditions. A total of 1,160 proteins were identified and those related to pathogenesis and stress response pathways were analyzed. The proteins involved in the expression of virulent pathways when L. monocytogenes ST7 strain was grown under different stress conditions were described. Certain proteins, particularly those involved in the pathogenesis pathway, such as Listeriolysin regulatory protein and Internalin A, were only found when the strain was grown under specific stress conditions. Studying how L. monocytogenes adapts to stress can help to control its growth in food, reducing the risk for consumers. MDPI 2023-05-27 /pmc/articles/PMC10252599/ /pubmed/37297410 http://dx.doi.org/10.3390/foods12112166 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article D’Onofrio, Federica Schirone, Maria Paparella, Antonello Krasteva, Ivanka Tittarelli, Manuela Pomilio, Francesco Iannetti, Luigi D’Alterio, Nicola Luciani, Mirella Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling |
title | Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling |
title_full | Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling |
title_fullStr | Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling |
title_full_unstemmed | Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling |
title_short | Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling |
title_sort | stress adaptation responses of a listeria monocytogenes 1/2a strain via proteome profiling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252599/ https://www.ncbi.nlm.nih.gov/pubmed/37297410 http://dx.doi.org/10.3390/foods12112166 |
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