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Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers

Cow’s milk allergy (CMA) is one of the most prevalent food allergies in children. Several studies have demonstrated that gut microbiota influences the acquisition of oral tolerance to food antigens at initial stages of life. Changes in the gut microbiota composition and/or functionality (i.e., dysbi...

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Autores principales: Zubeldia-Varela, Elisa, Barker-Tejeda, Tomás Clive, Mera-Berriatua, Leticia, Bazire, Raphaëlle, Cabrera-Freitag, Paula, Ubeda, Carles, Barber, Domingo, Francino, María Pilar, Rojo, David, Ibáñez-Sandín, María Dolores, Pérez-Gordo, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252606/
https://www.ncbi.nlm.nih.gov/pubmed/37298198
http://dx.doi.org/10.3390/ijms24119247
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author Zubeldia-Varela, Elisa
Barker-Tejeda, Tomás Clive
Mera-Berriatua, Leticia
Bazire, Raphaëlle
Cabrera-Freitag, Paula
Ubeda, Carles
Barber, Domingo
Francino, María Pilar
Rojo, David
Ibáñez-Sandín, María Dolores
Pérez-Gordo, Marina
author_facet Zubeldia-Varela, Elisa
Barker-Tejeda, Tomás Clive
Mera-Berriatua, Leticia
Bazire, Raphaëlle
Cabrera-Freitag, Paula
Ubeda, Carles
Barber, Domingo
Francino, María Pilar
Rojo, David
Ibáñez-Sandín, María Dolores
Pérez-Gordo, Marina
author_sort Zubeldia-Varela, Elisa
collection PubMed
description Cow’s milk allergy (CMA) is one of the most prevalent food allergies in children. Several studies have demonstrated that gut microbiota influences the acquisition of oral tolerance to food antigens at initial stages of life. Changes in the gut microbiota composition and/or functionality (i.e., dysbiosis) have been linked to inadequate immune system regulation and the emergence of pathologies. Moreover, omic sciences have become an essential tool for the analysis of the gut microbiota. On the other hand, the use of fecal biomarkers for the diagnosis of CMA has recently been reviewed, with fecal calprotectin, α-1 antitrypsin, and lactoferrin being the most relevant. This study aimed at evaluating functional changes in the gut microbiota in the feces of cow’s milk allergic infants (AI) compared to control infants (CI) by metagenomic shotgun sequencing and at correlating these findings with the levels of fecal biomarkers (α-1 antitrypsin, lactoferrin, and calprotectin) by an integrative approach. We have observed differences between AI and CI groups in terms of fecal protein levels and metagenomic analysis. Our findings suggest that AI have altered glycerophospholipid metabolism as well as higher levels of lactoferrin and calprotectin that could be explained by their allergic status.
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spelling pubmed-102526062023-06-10 Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers Zubeldia-Varela, Elisa Barker-Tejeda, Tomás Clive Mera-Berriatua, Leticia Bazire, Raphaëlle Cabrera-Freitag, Paula Ubeda, Carles Barber, Domingo Francino, María Pilar Rojo, David Ibáñez-Sandín, María Dolores Pérez-Gordo, Marina Int J Mol Sci Article Cow’s milk allergy (CMA) is one of the most prevalent food allergies in children. Several studies have demonstrated that gut microbiota influences the acquisition of oral tolerance to food antigens at initial stages of life. Changes in the gut microbiota composition and/or functionality (i.e., dysbiosis) have been linked to inadequate immune system regulation and the emergence of pathologies. Moreover, omic sciences have become an essential tool for the analysis of the gut microbiota. On the other hand, the use of fecal biomarkers for the diagnosis of CMA has recently been reviewed, with fecal calprotectin, α-1 antitrypsin, and lactoferrin being the most relevant. This study aimed at evaluating functional changes in the gut microbiota in the feces of cow’s milk allergic infants (AI) compared to control infants (CI) by metagenomic shotgun sequencing and at correlating these findings with the levels of fecal biomarkers (α-1 antitrypsin, lactoferrin, and calprotectin) by an integrative approach. We have observed differences between AI and CI groups in terms of fecal protein levels and metagenomic analysis. Our findings suggest that AI have altered glycerophospholipid metabolism as well as higher levels of lactoferrin and calprotectin that could be explained by their allergic status. MDPI 2023-05-25 /pmc/articles/PMC10252606/ /pubmed/37298198 http://dx.doi.org/10.3390/ijms24119247 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zubeldia-Varela, Elisa
Barker-Tejeda, Tomás Clive
Mera-Berriatua, Leticia
Bazire, Raphaëlle
Cabrera-Freitag, Paula
Ubeda, Carles
Barber, Domingo
Francino, María Pilar
Rojo, David
Ibáñez-Sandín, María Dolores
Pérez-Gordo, Marina
Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
title Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
title_full Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
title_fullStr Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
title_full_unstemmed Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
title_short Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
title_sort further insights into the gut microbiota of cow’s milk allergic infants: analysis of microbial functionality and its correlation with three fecal biomarkers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252606/
https://www.ncbi.nlm.nih.gov/pubmed/37298198
http://dx.doi.org/10.3390/ijms24119247
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