Cargando…
Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers
Cow’s milk allergy (CMA) is one of the most prevalent food allergies in children. Several studies have demonstrated that gut microbiota influences the acquisition of oral tolerance to food antigens at initial stages of life. Changes in the gut microbiota composition and/or functionality (i.e., dysbi...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252606/ https://www.ncbi.nlm.nih.gov/pubmed/37298198 http://dx.doi.org/10.3390/ijms24119247 |
_version_ | 1785056211101548544 |
---|---|
author | Zubeldia-Varela, Elisa Barker-Tejeda, Tomás Clive Mera-Berriatua, Leticia Bazire, Raphaëlle Cabrera-Freitag, Paula Ubeda, Carles Barber, Domingo Francino, María Pilar Rojo, David Ibáñez-Sandín, María Dolores Pérez-Gordo, Marina |
author_facet | Zubeldia-Varela, Elisa Barker-Tejeda, Tomás Clive Mera-Berriatua, Leticia Bazire, Raphaëlle Cabrera-Freitag, Paula Ubeda, Carles Barber, Domingo Francino, María Pilar Rojo, David Ibáñez-Sandín, María Dolores Pérez-Gordo, Marina |
author_sort | Zubeldia-Varela, Elisa |
collection | PubMed |
description | Cow’s milk allergy (CMA) is one of the most prevalent food allergies in children. Several studies have demonstrated that gut microbiota influences the acquisition of oral tolerance to food antigens at initial stages of life. Changes in the gut microbiota composition and/or functionality (i.e., dysbiosis) have been linked to inadequate immune system regulation and the emergence of pathologies. Moreover, omic sciences have become an essential tool for the analysis of the gut microbiota. On the other hand, the use of fecal biomarkers for the diagnosis of CMA has recently been reviewed, with fecal calprotectin, α-1 antitrypsin, and lactoferrin being the most relevant. This study aimed at evaluating functional changes in the gut microbiota in the feces of cow’s milk allergic infants (AI) compared to control infants (CI) by metagenomic shotgun sequencing and at correlating these findings with the levels of fecal biomarkers (α-1 antitrypsin, lactoferrin, and calprotectin) by an integrative approach. We have observed differences between AI and CI groups in terms of fecal protein levels and metagenomic analysis. Our findings suggest that AI have altered glycerophospholipid metabolism as well as higher levels of lactoferrin and calprotectin that could be explained by their allergic status. |
format | Online Article Text |
id | pubmed-10252606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102526062023-06-10 Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers Zubeldia-Varela, Elisa Barker-Tejeda, Tomás Clive Mera-Berriatua, Leticia Bazire, Raphaëlle Cabrera-Freitag, Paula Ubeda, Carles Barber, Domingo Francino, María Pilar Rojo, David Ibáñez-Sandín, María Dolores Pérez-Gordo, Marina Int J Mol Sci Article Cow’s milk allergy (CMA) is one of the most prevalent food allergies in children. Several studies have demonstrated that gut microbiota influences the acquisition of oral tolerance to food antigens at initial stages of life. Changes in the gut microbiota composition and/or functionality (i.e., dysbiosis) have been linked to inadequate immune system regulation and the emergence of pathologies. Moreover, omic sciences have become an essential tool for the analysis of the gut microbiota. On the other hand, the use of fecal biomarkers for the diagnosis of CMA has recently been reviewed, with fecal calprotectin, α-1 antitrypsin, and lactoferrin being the most relevant. This study aimed at evaluating functional changes in the gut microbiota in the feces of cow’s milk allergic infants (AI) compared to control infants (CI) by metagenomic shotgun sequencing and at correlating these findings with the levels of fecal biomarkers (α-1 antitrypsin, lactoferrin, and calprotectin) by an integrative approach. We have observed differences between AI and CI groups in terms of fecal protein levels and metagenomic analysis. Our findings suggest that AI have altered glycerophospholipid metabolism as well as higher levels of lactoferrin and calprotectin that could be explained by their allergic status. MDPI 2023-05-25 /pmc/articles/PMC10252606/ /pubmed/37298198 http://dx.doi.org/10.3390/ijms24119247 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zubeldia-Varela, Elisa Barker-Tejeda, Tomás Clive Mera-Berriatua, Leticia Bazire, Raphaëlle Cabrera-Freitag, Paula Ubeda, Carles Barber, Domingo Francino, María Pilar Rojo, David Ibáñez-Sandín, María Dolores Pérez-Gordo, Marina Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers |
title | Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers |
title_full | Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers |
title_fullStr | Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers |
title_full_unstemmed | Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers |
title_short | Further Insights into the Gut Microbiota of Cow’s Milk Allergic Infants: Analysis of Microbial Functionality and Its Correlation with Three Fecal Biomarkers |
title_sort | further insights into the gut microbiota of cow’s milk allergic infants: analysis of microbial functionality and its correlation with three fecal biomarkers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252606/ https://www.ncbi.nlm.nih.gov/pubmed/37298198 http://dx.doi.org/10.3390/ijms24119247 |
work_keys_str_mv | AT zubeldiavarelaelisa furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT barkertejedatomasclive furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT meraberriatualeticia furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT bazireraphaelle furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT cabrerafreitagpaula furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT ubedacarles furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT barberdomingo furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT francinomariapilar furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT rojodavid furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT ibanezsandinmariadolores furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers AT perezgordomarina furtherinsightsintothegutmicrobiotaofcowsmilkallergicinfantsanalysisofmicrobialfunctionalityanditscorrelationwiththreefecalbiomarkers |