Cargando…

MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae

Calcineurin, a key regulator of the calcium signaling pathway, is involved in calcium signal transduction and calcium ion homeostasis. Magnaporthe oryzae is a devastating filamentous phytopathogenic fungus in rice, yet little is known about the function of the calcium signaling system. Here, we iden...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Zi-He, Shen, Zi-Fang, Wang, Jing-Yi, Cai, Ying-Ying, Li, Lin, Liao, Jian, Lu, Jian-Ping, Zhu, Xue-Ming, Lin, Fu-Cheng, Liu, Xiao-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252744/
https://www.ncbi.nlm.nih.gov/pubmed/37298247
http://dx.doi.org/10.3390/ijms24119297
_version_ 1785056243855917056
author Wang, Zi-He
Shen, Zi-Fang
Wang, Jing-Yi
Cai, Ying-Ying
Li, Lin
Liao, Jian
Lu, Jian-Ping
Zhu, Xue-Ming
Lin, Fu-Cheng
Liu, Xiao-Hong
author_facet Wang, Zi-He
Shen, Zi-Fang
Wang, Jing-Yi
Cai, Ying-Ying
Li, Lin
Liao, Jian
Lu, Jian-Ping
Zhu, Xue-Ming
Lin, Fu-Cheng
Liu, Xiao-Hong
author_sort Wang, Zi-He
collection PubMed
description Calcineurin, a key regulator of the calcium signaling pathway, is involved in calcium signal transduction and calcium ion homeostasis. Magnaporthe oryzae is a devastating filamentous phytopathogenic fungus in rice, yet little is known about the function of the calcium signaling system. Here, we identified a novel calcineurin regulatory-subunit-binding protein, MoCbp7, which is highly conserved in filamentous fungi and was found to localize in the cytoplasm. Phenotypic analysis of the MoCBP7 gene deletion mutant (ΔMocbp7) showed that MoCbp7 influenced the growth, conidiation, appressorium formation, invasive growth, and virulence of M. oryzae. Some calcium-signaling-related genes, such as YVC1, VCX1, and RCN1, are expressed in a calcineurin/MoCbp7-dependent manner. Furthermore, MoCbp7 synergizes with calcineurin to regulate endoplasmic reticulum homeostasis. Our research indicated that M. oryzae may have evolved a new calcium signaling regulatory network to adapt to its environment compared to the fungal model organism Saccharomyces cerevisiae.
format Online
Article
Text
id pubmed-10252744
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102527442023-06-10 MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae Wang, Zi-He Shen, Zi-Fang Wang, Jing-Yi Cai, Ying-Ying Li, Lin Liao, Jian Lu, Jian-Ping Zhu, Xue-Ming Lin, Fu-Cheng Liu, Xiao-Hong Int J Mol Sci Article Calcineurin, a key regulator of the calcium signaling pathway, is involved in calcium signal transduction and calcium ion homeostasis. Magnaporthe oryzae is a devastating filamentous phytopathogenic fungus in rice, yet little is known about the function of the calcium signaling system. Here, we identified a novel calcineurin regulatory-subunit-binding protein, MoCbp7, which is highly conserved in filamentous fungi and was found to localize in the cytoplasm. Phenotypic analysis of the MoCBP7 gene deletion mutant (ΔMocbp7) showed that MoCbp7 influenced the growth, conidiation, appressorium formation, invasive growth, and virulence of M. oryzae. Some calcium-signaling-related genes, such as YVC1, VCX1, and RCN1, are expressed in a calcineurin/MoCbp7-dependent manner. Furthermore, MoCbp7 synergizes with calcineurin to regulate endoplasmic reticulum homeostasis. Our research indicated that M. oryzae may have evolved a new calcium signaling regulatory network to adapt to its environment compared to the fungal model organism Saccharomyces cerevisiae. MDPI 2023-05-26 /pmc/articles/PMC10252744/ /pubmed/37298247 http://dx.doi.org/10.3390/ijms24119297 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Zi-He
Shen, Zi-Fang
Wang, Jing-Yi
Cai, Ying-Ying
Li, Lin
Liao, Jian
Lu, Jian-Ping
Zhu, Xue-Ming
Lin, Fu-Cheng
Liu, Xiao-Hong
MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
title MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
title_full MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
title_fullStr MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
title_full_unstemmed MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
title_short MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
title_sort mocbp7, a novel calcineurin b subunit-binding protein, is involved in the calcium signaling pathway and regulates fungal development, virulence, and er homeostasis in magnaporthe oryzae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252744/
https://www.ncbi.nlm.nih.gov/pubmed/37298247
http://dx.doi.org/10.3390/ijms24119297
work_keys_str_mv AT wangzihe mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT shenzifang mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT wangjingyi mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT caiyingying mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT lilin mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT liaojian mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT lujianping mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT zhuxueming mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT linfucheng mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae
AT liuxiaohong mocbp7anovelcalcineurinbsubunitbindingproteinisinvolvedinthecalciumsignalingpathwayandregulatesfungaldevelopmentvirulenceanderhomeostasisinmagnaportheoryzae