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Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis

BACKGROUND: Coronary atherosclerosis diseases (CADs) are associated with chronic inflammation. Neutrophil extracellular traps (NETs) are a type of novel proinflammatory cytokines whose levels are dramatically elevated in acute coronary syndrome. We conducted this study to further evaluate the associ...

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Autores principales: Wang, Yan, Yang, Mao, Xu, Yuanyuan, Yan, Shujun, Jin, Enze, Li, Xueqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252804/
https://www.ncbi.nlm.nih.gov/pubmed/37304868
http://dx.doi.org/10.7717/peerj.15471
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author Wang, Yan
Yang, Mao
Xu, Yuanyuan
Yan, Shujun
Jin, Enze
Li, Xueqi
author_facet Wang, Yan
Yang, Mao
Xu, Yuanyuan
Yan, Shujun
Jin, Enze
Li, Xueqi
author_sort Wang, Yan
collection PubMed
description BACKGROUND: Coronary atherosclerosis diseases (CADs) are associated with chronic inflammation. Neutrophil extracellular traps (NETs) are a type of novel proinflammatory cytokines whose levels are dramatically elevated in acute coronary syndrome. We conducted this study to further evaluate the association between circulating NET-associated markers and CAD in Chinese adults. METHODS: A total of 174 patients with CAD and 55 healthy controls were screened using percutaneous coronary intervention or coronary computed tomography angiography. Blood lipid levels, blood glucose levels, and blood cell counts were determined using commercial kits. Serum levels of myeloperoxidase (MPO) and neutrophil elastase (NE) were measured using ELISA. Double-stranded DNA (dsDNA) in serum was quantified using the Quant-iT PicoGreen assay. We also compared the circulating NET levels with various parameters in the study subjects. RESULTS: The levels of serum NET markers, dsDNA, MPO, and NE, were significantly elevated in patients with CAD, particularly in the severe group, consistent with the increase in neutrophil counts. The levels of NET markers correlated with the risk factors of AS, increasing with the number of risk factors. NET markers were identified as independent risk factors for severe coronary stenosis and also as predictors of severe CAD. CONCLUSION: NETs may be related to AS and serve as indicators or predictors of stenosis in patients with severe CAD.
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spelling pubmed-102528042023-06-10 Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis Wang, Yan Yang, Mao Xu, Yuanyuan Yan, Shujun Jin, Enze Li, Xueqi PeerJ Cardiology BACKGROUND: Coronary atherosclerosis diseases (CADs) are associated with chronic inflammation. Neutrophil extracellular traps (NETs) are a type of novel proinflammatory cytokines whose levels are dramatically elevated in acute coronary syndrome. We conducted this study to further evaluate the association between circulating NET-associated markers and CAD in Chinese adults. METHODS: A total of 174 patients with CAD and 55 healthy controls were screened using percutaneous coronary intervention or coronary computed tomography angiography. Blood lipid levels, blood glucose levels, and blood cell counts were determined using commercial kits. Serum levels of myeloperoxidase (MPO) and neutrophil elastase (NE) were measured using ELISA. Double-stranded DNA (dsDNA) in serum was quantified using the Quant-iT PicoGreen assay. We also compared the circulating NET levels with various parameters in the study subjects. RESULTS: The levels of serum NET markers, dsDNA, MPO, and NE, were significantly elevated in patients with CAD, particularly in the severe group, consistent with the increase in neutrophil counts. The levels of NET markers correlated with the risk factors of AS, increasing with the number of risk factors. NET markers were identified as independent risk factors for severe coronary stenosis and also as predictors of severe CAD. CONCLUSION: NETs may be related to AS and serve as indicators or predictors of stenosis in patients with severe CAD. PeerJ Inc. 2023-06-06 /pmc/articles/PMC10252804/ /pubmed/37304868 http://dx.doi.org/10.7717/peerj.15471 Text en ©2023 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cardiology
Wang, Yan
Yang, Mao
Xu, Yuanyuan
Yan, Shujun
Jin, Enze
Li, Xueqi
Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
title Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
title_full Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
title_fullStr Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
title_full_unstemmed Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
title_short Neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
title_sort neutrophil extracellular trap burden correlates with the stenosis of coronary atherosclerosis
topic Cardiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252804/
https://www.ncbi.nlm.nih.gov/pubmed/37304868
http://dx.doi.org/10.7717/peerj.15471
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